Diversity and population structure of black soybean landraces originating from Tanba and neighboring regions

Abstract

Black soybean landraces that had been cultivated in Tanba region and the neighboring regions and conserved black soybean landraces, including those from other regions in Japan, were used in this study. The polymorphisms of 78 SSR markers in nuclear DNA and 6 SSRs in chloroplast DNA were analyzed in the black soybean landrace populations. The result of phylogenic analysis revealed that the black soybeans can be classified into six clades. The landraces originating from Tanba region were classed into first and second clades, and two chloroplast genotypes were found in the population of black soybeans from the Tanba region. Genotype A chloroplast was predominantly identified in major populations of the Tanba, while genotype B was widely distributed in the black soybean population. Population structure analysis in the Japanese black soybean accessions inferred there are six groups. The black soybean landrace from the Tanba region was classified into three groups, mainly corresponding to the distance-based phylogenic results. The two groups were probably derived from different ancestors with Type A and B chloroplast genomes, respectively, whereas the other group showed both types of chloroplast genome. The admixture situations suggested that the landraces in the main group have been widely cultivated in Tanba region, while the landraces that belong to other groups were cultivated in localized area. Several phenotypes were compared among genotype groups, dividing into two sub-groups: founder sub-group and admixed sub-group. Phenotypic differences were observed between founder landraces in group 1 and group 3. On the other hand, landraces in admixture landraces in group 1 and group 2 segregated for several traits, while founder landraces in group 1 were stabled for each trait. These observations suggest that gene flow events have occurred between different founder landraces.

Genetic diversity analysis of soybean (Glycine max (L.) Merr.) genotypes making use of SSR markers

“…A previous study on revolutionary relationship between Glycine soja and Glycine max revealed two clusters (Wen et al, 2009). The results of the present study are consistent with the findings of Wang et al (2006), Wen et al (2008), Hirota et al (2012), Ghosh et al (2014), and Bisen et al (2015). The genotypes used in this study showed genetic variation because of the different source and/or area from which they were collected; hence, they belonged to different clusters.…”

Genome Re-Sequencing of Semi-Wild Soybean Reveals a Complex Soja Population Structure and Deep Introgression

“…The hybridization origin of the semi-wild soybean has been proposed [13] , [60] . However, direct evidence in support of this hypothesis is lacking, although hybridization events between cultivated and wild soybean have been observed in natural field conditions [6] , [18] , [19] .…”

Cell wall composition and nutrients in soybean genotypesof varying seed coat colour

“…Among all the genotypes SL781 and SEL7 exhibited maximum (0.118g) and minimum (0.055g) weight/ seed. A wide variation for 100 seed weight has been reported by various authors among soybean seeds varying in seed coat colour (Zhang et al, 2010;Hirota et al, 2012;Goyal et al, 2012;Sharma et al, 2014 green and yellow coloured groups of soybean genotypes, respectively (Fig 1). Among black coloured soybean genotypes, dry matter was highest in SL783 and lowest in SEL37.…”