2012
DOI: 10.1371/journal.pone.0043135
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Abstract: BackgroundThis study is the first to investigate the Brazilian Amazonian Forest to identify new D-xylose-fermenting yeasts that might potentially be used in the production of ethanol from sugarcane bagasse hemicellulosic hydrolysates.Methodology/Principal FindingsA total of 224 yeast strains were isolated from rotting wood samples collected in two Amazonian forest reserve sites. These samples were cultured in yeast nitrogen base (YNB)-D-xylose or YNB-xylan media. Candida tropicalis, Asterotremella humicola, Ca… Show more

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Cited by 115 publications
(115 citation statements)
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“…The tubes were incubated on an orbital shaker under the same conditions described above. After growth was detected, one loopful from each tube was streaked on YNB-D-xylose, YNB-xylan or YNB-CMC agar media (Cadete et al 2012). The plates were incubated at 25°C until colonies developed.…”
Section: Collection Area and Yeast Isolationmentioning
confidence: 99%
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“…The tubes were incubated on an orbital shaker under the same conditions described above. After growth was detected, one loopful from each tube was streaked on YNB-D-xylose, YNB-xylan or YNB-CMC agar media (Cadete et al 2012). The plates were incubated at 25°C until colonies developed.…”
Section: Collection Area and Yeast Isolationmentioning
confidence: 99%
“…Conspecific isolates generally produce identical banding profiles using this PCRfingerprinting technique (Rosa et al 2007). Yeast strains with identical PCR fingerprinting patterns were grouped and putatively considered to belong to the same species (Cadete et al 2012). At least one representative strain from each EI1 group was subjected to sequence analysis of the D1/D2 region of the large subunit (LSU) of the rRNA gene and the ITS-5.8S rRNA gene region, directly from whole cells, as described previously (Lachance et al 1999).…”
Section: Yeast Identificationmentioning
confidence: 99%
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“…During cofermentation, a variation of 89-95.44% was obtained (Table 4), which seems promising. Considering the results of Cadete et al (2012), who, with S. passalidarum in a medium with D-xylose (50 g.L -1 ) as the exclusive carbon source, obtained the maximum ethanol production in 24 h at a temperature of 30°C, with ethanol production ranging from 15 to 18 g.L -1 (Q P = 0.6 to 0.75 g.L.h -1…”
Section: Discussionmentioning
confidence: 99%
“…In fact, the gut of beetles and other insects is considered a hotspot of yeast diversity (Suh and Blackwell, 2004;Boekhout, 2005;Rivera et al, 2009;Cadete et al, 2009Cadete et al, , 2012Urbina et al, 2013;Gouliamova et al, 2015;Cadete et al, 2015Cadete et al, , 2016.…”
Section: Introductionmentioning
confidence: 99%