Diversity and Functional Potential of Soil Bacterial Communities in Different Types of Farmland Shelterbelts in Mid-Western Heilongjiang, China

Abstract

The diversity and function of surface soil bacterial community in farmland shelterbelts of five forest types and one abandoned wilderness area were analyzed by collecting 36 soil samples at depths of 0–10 cm (the upper soil layer) and 10–20 cm (the lower soil layer), extracting DNA from the samples and amplifying and sequencing the bacterial 16S rDNA V3~V4 region. Dominant bacterial phyla in forest soils included the Actinomycetes, Proteobacteria, Acidobacteria, Chlorobacteria, and Bacillus. The number of unique bacterial OTUs (operational taxonomic units) was higher in the upper versus lower soil layer and greater in the abandoned cropland than in the shelterbelts. The number of total bacterial OTUs was highest in the mixed Pinus sylvestris var. mongholica Litv. and Larix gmelinii (Rupr.) Kuzen. forest. At the phyla level, Actinomycetes showed the greatest variation in abundance among forest types, while at the genus level, Actinoplanes varied most among forest types in the upper soil layer and Krasilnikovia varied most in the lower soil layer. Soil bacteria were more strongly correlated and more intense competition in the upper soil layer than in the lower soil layer; Actinoplanes and Krasilnikovia were key genera in bacterial networks. Functional predictions for bacterial community genes indicated that soil fertility potential was strong in the mixed Fraxinus mandshurica Rupr. and Larix gmelinii (Rupr.) Kuzen. forest, weak in the mixed Pinus sylvestris var. mongholica Litv. and Larix gmelinii (Rupr.) Kuzen. forest, and in the Populus×xiaohei forest, and intermediate in the Larix gmelinii (Rupr.) Kuzen. and Pinus sylvestris var. mongholica Litv. forests. This study provides a new theoretical basis for the sustainable management of soil fertility in the agroforestry system.

Recombination of endophytic bacteria in asexual plant Ligusticum chuanxiong Hort. caused by transplanting

“…It inhibits plant diseases by producing antibiotics, and promotes plant growth by dissolving phosphate and generating secondary metabolites ( Franco-Correa et al, 2010 ; Palaniyandi et al, 2013 ). Micromonosporaceae is widely distributed in nature, including terrestrial, aquatic and plant endophytic environments ( Zhang, Xin & Zhao, 2019 ; Ramesh & William, 2013 ; Nattakorn et al, 2018 ). Micromonosporaceae can produce aminoglycosides or alkaloids and other antibiotics ( Armstrong et al., 2012 ; Charan et al, 2004 ), which can inhibit gram-positive bacteria or viruses ( Wagman, 1980 ).…”

Impacts of shelterbelt systems on pasture production and soil bacterial and fungal communities in agricultural fields

“…Only a few studies have estimated the effects of windbreaks on microbial activities (e.g., soil respiration, nitrogen mineralisation and dehydrogenase activity) and the total number of bacteria and fungi using culture plates in Poland (Wojewoda & Russel, 2003). Other studies have focused on specific bacterial (Actinomycetales) and fungal individuals near black locust shelterbelts on the Proszowicki Plateau (Dłużniewska & Mazurek, 2011), using quantitative PCR and terminal restriction fragment length polymorphism (TRFLP) in Australia (Carnovale et al, 2019), or soil bacterial communities in Northeast China (Zhang et al, 2019). Given that soil bacteria and fungi strongly respond to climate change (Trivedi et al, 2022) and plant species (Bulgarelli et al, 2012), understanding the influences of shelterbelts on soil microbial communities are crucial to maintain their activities and roles in performing soil functions and benefiting plants.…”