Diversity and Frequencies of HLA Class I and Class II Genes of an East African Population

Peterson, Trevor; Bielawny, Thomas; Lacap, Philip; Hardie, Rae-Anne; Daniuk, Christina; Mendoza, Luís Hurtado de; Thavaneswaran, Subotheni; Kariri, T.; Kimani, Joshua; Wachihi, Charles; Kimani, Maboku; Ball, T. Blake; Plummer, Francis A.; Luo, Ma

doi:10.4236/ojgen.2014.42013

Cited by 11 publications

(9 citation statements)

References 26 publications

(22 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Comparing the results with other studies, the allele frequency observed in Group 2 was similar to other studies performed with Caucasian populations (Delfino et al ., ; Moya‐Quiles et al ., ; Balas et al ., ). On the other hand, the frequency observed in Group 3 can be compared with African descent populations (Nigam et al ., ; Tu et al ., ; Peterson et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Allele and haplotype frequencies of HLA‐A, B, C, DRB1 and DQB1 genes in polytransfused patients in ethnically diverse populations from Brazil

Rodrigues

Macedo

Bruder

et al. 2015

Int J Immunogenetics

View full text Add to dashboard Cite

The red blood transfusion is a practice often used in patients with haematological and oncological diseases. However, the investigation of human leucocyte antigen (HLA) system frequency in these individuals is of great importance because multiple transfusions may lead to HLA alloimmunization. Brazil is a country that was colonized by many other ethnicities, leading to a mixed ethnicity and regionalized population. In view of the importance of HLA typing in these patients, the aim of this study was to investigate the allele and haplotype frequencies from polytransfused patients from three different regions from Brazil. HLA-A, HLA-B, HLA-C, HLA-DRB1 and HLA-DQB1 genotyping of 366 patients was performed by PCR-SSO, based on the Luminex technology (One Lambda(®) ), and the anti-HLA class I and class II antibodies were analysed using LabScreen Single Antigen Antibody Detection (One Lambda, Inc.). Allele and haplotype frequencies of polytransfused patients of three regions from Brazil were obtained using the Arlequin program. The most frequent allele frequencies observed were HLA-A*02, A*03, B*15, B*35, B*51, C*07, C*04, C*03, DRB1*13, DRB1*11, DRB1*07, DRB1*03, DRB1*01, DQB1*03, DQB1*02, DQB1*06 and DQB1*05. There were differences between the groups for allele variants HLA-B*57 (between Group 1 and Group 2) and HLA-C*12 (between Group 1 and Group 3). The most frequent haplotypes found in the sample were HLA-A*01B*08DRB1*03, DRBI*07DQB1*02, DRB1*01DQB1*05, DRB1*13DQB1*06 and A*02B*35. HLA class I and II antibodies were detected in 77.9% and 63.9% patients, respectively, while the both alloantibodies were detected in 62 (50.9%) patients. In conclusion, the HLA typing for polytransfused patients in each region has a great importance, as seen in this study; individuals from different regions from Brazil have HLA distribution not completely homogeneous.

show abstract

Section: Discussionmentioning

confidence: 97%

Allele and haplotype frequencies of HLA‐A, B, C, DRB1 and DQB1 genes in polytransfused patients in ethnically diverse populations from Brazil

Rodrigues

Macedo

Bruder

et al. 2015

Int J Immunogenetics

View full text Add to dashboard Cite

show abstract

“…[32][33][34] On the other hand, the frequency of DQ2 was higher than the results of studies from Iran (20%), Turkey (18%), Southern India (12.78%), Rwanda (15.5%), Tanzania (13.5%) and Cameroon (7.0%), [32][33][34] but lower compared with a study on the Saharawi population (39%), where the prevalence of the celiac disease has been estimated to be the highest in the world. 35 Our study also showed that the DQ8 haplotype was less frequently found among the Ethiopian children as compared with the Swedish references (6.8% vs 13.1%), but comparable with study findings from Japan (7.6%), Sardinia (5%) 33 and 8.9% of Caucasians in the United States. 36 In addition, we found a higher distribution of DQ8 than in South Africa (2.8%), Saharawi (2.7%), Algeria (2.2%), Italy (2%) and Cameroon (0.6%).…”

Section: Discussionmentioning

confidence: 99%

Distribution of HLA‐DQ risk genotypes for celiac disease in Ethiopian children

Gudeta

Ramelius

Balcha

et al. 2020

HLA

View full text Add to dashboard Cite

Most patients with celiac disease are positive for either HLA-DQA1*05: 01-DQB1*02 (DQ2.5) or DQA1*03:01-DQB1*03:02 (DQ8). Remaining few patients are usually DQA1*02:01-DQB1*02 (DQ2.2) carriers. Screenings of populations with high frequencies of these HLA-DQA1-DQB1 haplotypes report a 1% to 3% celiac disease prevalence. The aim was to determine the prevalence of HLA-DQ risk haplotypes for celiac disease in Ethiopian children. Dried blood spots collected from 1193 children from the Oromia regional state of Ethiopia were genotyped for HLA-DQA1 and DQB1 genotyping using an asymmetric polymerase chain reaction (PCR) and a subsequent hybridization of allele-specific probes. As references, 2000 previously HLA-genotyped children randomly selected from the general population in Sweden were included. DQ2.2 was the most common haplotype and found in 15.3% of Ethiopian children, which was higher compared with 6.7% of Swedish references (P < .0001). Opposed to this finding, DQ2.5 and DQ8 occurred in 9.7% and 6.8% of Ethiopian children, which were less frequent compared with 12.8% and 13.1% of Swedish references, respectively (P < .0001). The DQ2.5-trans genotype encoded by DQA1*05-DQB1*03:01 in combination with DQ2.2 occurred in 3.6% of Ethiopian children, which was higher compared with 1.3% of Swedish references (P < .0001). However, when children with moderate high to very high-risk HLA genotypes were grouped together, there was no difference between Ethiopian children and Swedish references (27.4% vs 29.0%) (P = .3504). The frequency of HLA risk haplotypes for celiac disease is very similar in Ethiopian and Swedish children. This finding of importance will be useful in future screening of children for celiac disease in Ethiopia.

show abstract

“…It was previously demonstrated that cross-reactive memory B-cell responses were primed after 2009 pdmH1N1 infection [ 29 ] and activation of NP-specific T-cell response by MVA-NP+M1 in human NALT [ 30 ]. Because M1 contains major immunodominant CD8 + T-cell epitopes and HLA-A2 is among the most common HLA alleles (20%–30%) [ 31 ], we examined HLA-A2 restricted M1 58–66 peptide-specific CD8 + T-cell responses in adenotonsillar tissue after MVA-NP+M1 stimulation. We showed that MVA-NP+M1 elicits marked increases in M1-specific CD8 + T cells, including T RM cells, which exhibit rapid degranulation and target cell killing on recall antigen recognition.…”

mentioning

confidence: 99%

Modified Vaccinia Ankara–Vectored Vaccine Expressing Nucleoprotein and Matrix Protein 1 (M1) Activates Mucosal M1-Specific T-Cell Immunity and Tissue-Resident Memory T Cells in Human Nasopharynx-Associated Lymphoid Tissue

Puksuriwong

Ahmed

Sharma

et al. 2019

The Journal of Infectious Diseases

View full text Add to dashboard Cite

Increasing evidences support a critical role of CD8+ T cell immunity against influenza. Activation of mucosal CD8+T cells, particularly tissue-resident memory T(TRM) cells recognizing conserved epitopes would mediate rapid and broad protection. Matrix protein 1(M1) is a well-conserved internal protein. We studied the capacity of Modified Vaccinia Ankara-vectored vaccine expressing nucleoprotein(NP) and M1(MVA-NP+M1) to activate M1-specific CD8+ T cell response including TRM cells in nasopharynx-associated lymphoid tissue(NALT) from children and adults. Following MVA-NP+M1 stimulation, M1 was abundantly expressed in adenotonsillar epithelial cells and B cells. MVA-NP+M1 activated marked IFN-γ-secreting T cell response to M1 peptides. Using tetramer staining, we showed the vaccine activated a marked increase in M158-66-specific CD8+ T cells in tonsillar mononuclear cells (MNC) of HLA-matched individuals. We also demonstrated MVA-NP+M1 activated a substantial increase in TRM cells exhibiting effector memory T cell phenotype. Upon recall antigen recognition, M1-specific T cells rapidly undergo cytotoxic degranulation, release granzyme B and pro-inflammatory cytokines, leading to target cell killing. Conclusion: MVA-NP+M1 elicits a substantial M1-specific T cell response including TRM cells in NALT, demonstrating its strong capacity to expand memory T cell pool exhibiting effector memory T cell phenotype, therefore offering great potential for rapid and broad protection against influenza reinfection.

show abstract

Diversity and Frequencies of HLA Class I and Class II Genes of an East African Population

Cited by 11 publications

References 26 publications

Allele and haplotype frequencies of HLA‐A, B, C, DRB1 and DQB1 genes in polytransfused patients in ethnically diverse populations from Brazil

Allele and haplotype frequencies of HLA‐A, B, C, DRB1 and DQB1 genes in polytransfused patients in ethnically diverse populations from Brazil

Distribution of HLA‐DQ risk genotypes for celiac disease in Ethiopian children

Modified Vaccinia Ankara–Vectored Vaccine Expressing Nucleoprotein and Matrix Protein 1 (M1) Activates Mucosal M1-Specific T-Cell Immunity and Tissue-Resident Memory T Cells in Human Nasopharynx-Associated Lymphoid Tissue

Contact Info

Product

Resources

About