Diversion procto-colitis: Response to treatment with short-chain fatty acids

Kiely, Edward M.; Ajayi, N A; Wheeler, Robert; Malone, Marian

doi:10.1053/jpsu.2001.27034

Cited by 47 publications

(57 citation statements)

References 17 publications

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“…They gave the name "diversion colitis" to this new form of intestinal inflammatory disease. Since this initial description, studies have been demonstrating that the etiopathogenesis of diversion colitis is probably related to intraluminal deficiency of short-chain fatty acids (SCFAs), which are the main energy substrate for colon epithelial cells 2,3 .…”

Section: Introductionmentioning

confidence: 99%

“…This demonstrates that the disease follows its course with a variety of changes and that there is no characteristic pattern [2][3][4] . The most common finds include epithelial erosions or ulcerations, decreased length or modified architecture of the colon crypts, inflammatory infiltrate, diffuse nodular lymphoid hyperplasia, vascular congestion of the submucosa and depletion of the number of caliciform cells, with consequent modifications to the production of mucins by the mucous epithelium [2][3][4] .…”

Section: Introductionmentioning

confidence: 99%

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Quantification by computerized morphometry of tissue levels of sulfomucins and sialomucins in diversion colitis in rats

et al. 2010

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PURPOSE: To quantify the intensity of sulfomucin and sialomucin expression in the colon mucosa, by means of computer-assisted image processing, comparing segments with and without fecal stream and correlating with the duration of fecal transit exclusion. METHODS: Forty-five Wistar rats were subjected to diversion of the fecal stream in the left colon by means of constructing a proximal colostomy and distal mucosal fistula. They were distributed randomly into three experimental groups of 15 animals, of which 10 were subjected to colon diversion (experimental subgroup) and five were only subjected to laparotomy, without colon diversion (control subgroup). The three experimental groups were formed according to the sacrifice date, which was to be performed six weeks after the surgical procedure (Group A), 12 weeks (Group B) and 18 weeks (Group C). The sulfomucin and sialomucin expression in the colon mucosa was evaluated using the histochemical technique of high iron diamine-alcian blue (HID-AB). The tissue expression was quantified for each animal, in the segments with and without fecal stream, at a location where there were four complete contiguous crypts in two random fields, with the aid of the computer-assisted image analysis software. The final value was taken to be the mean reading from the two fields selected, in the segments with and without fecal stream. To compare the expressions of the two mucin subtypes in the segments with and without fecal stream, the paired Student t test was used. To analyze variance according to duration of exclusion, ANOVA with the Newman-Keuls post-test was used, setting the significance level at 5% (p<0.05). RESULTS: There were significant reductions in tissue sulfomucin and sialomucin content in the colon without fecal stream, independent of the duration of exclusion considered. There was increased tissue sulfomucin content and decreased tissue sialomucin in the segments without fecal stream, with increasing duration of exclusion. CONCLUSIONS: Diversion of the fecal transit decreased the tissue sulfomucin and sialomucin content in the segments without fecal stream. Notwithstanding the reduction in the levels of both subtypes of acid mucin in the segments without fecal stream, there was increased tissue sulfomucin content and decreased tissue sialomucin with increasing duration of intestinal diversion.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Quantification by computerized morphometry of tissue levels of sulfomucins and sialomucins in diversion colitis in rats

et al. 2010

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“…Prominent among these is a theory correlating the appearance of this disease with decreased quantities of short-chain fatty acids (SCFAs) in the intestinal lumen. SCFAs are the principal energy substrate for metabolizing colonocytes 5,6 . In a colon without transit, the absence of fiber coming from the diet impedes the formation of SCFAs and their consequent absorption and metabolic use by the epithelial cells of the colon wall 7 .…”

Section: Introductionmentioning

confidence: 99%

“…The main histopathological alterations found in DC cases relate to the presence of different degrees of inflammation of the colon wall 2,6 . Studies conducted among patients with DC have presented controversial results, thus demonstrating that this disease progresses with a variety of histopathological changes and that there is no characteristic pattern 2 .…”

Section: Introductionmentioning

confidence: 99%

“…Studies conducted among patients with DC have presented controversial results, thus demonstrating that this disease progresses with a variety of histopathological changes and that there is no characteristic pattern 2 . The most common alterations include mucosal erosion or ulceration, nonspecific chronic inflammatory processes, diffuse nodular lymphoid hyperplasia, cryptitis, inflammatory infiltrate in the lamina propria, mucin depletion in the mucosal epithelium, atrophy of the colon crypts and, more rarely, tissue dysplasia 2,6 . These alterations seem to increase in intensity as the length of time without intestinal transit increases, and they may be reversed once the intestinal transit is reestablished 6 .…”

Section: Introductionmentioning

confidence: 99%

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Evaluation by computerized morphometry of histopathological alterations of the colon wall in segments with and without intestinal transit in rats

et al. 2008

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Purpose:To evaluate histopathological alterations of the colon wall in segments with and without intestinal transit, by computer-assisted imaging, and to correlate these with the length of time diversion. Methods: Thirty male Wistar rats were subjected to intestinal transit diversion by a proximal colostomy and distal mucosa fistula. The animals were divided into three experimental groups according to how long after the initial surgical procedure they were sacrificed: six, twelve and eighteen weeks. Colon segments with and without transit were subjected to histopathological study. The variables colon crypt length, mucosal ulceration, muscle layer thickness of the muscularis mucosa, submucosa and muscularis propria, vascular congestion, number of caliciform cells, inflammatory grade and degree of inflammation, comparing the two colon segments in the different experimental groups were studied. Intestinal crypt length, muscle layer thickness of the mucosa, submucosa and muscularis propria and caliciform cells were measured by computer-assisted imaging method. Mean equality, variance analysis and correlation tests were used in the statistical analysis, and the significance level was set at 5%. Results:Comparison between segments with and without transit showed that the latter presented reduced length of colon crypts and increased muscle layer thickness of the muscularis mucosa, submucosa and muscularis propria. There were greater quantities of ulceration of the mucosal and greater degree of inflammation with increasing time without transit. Mucosal ulceration, submucosal vascular congestion, increased thickness of the submucosal and muscularis propria layers, presence of caliciform cells, inflammatory infiltrate and inflammatory grade correlated significantly with the length of time without transit. Conclusions: Histological alterations occurred in all layers of the colon wall, in the segments without intestinal transit. Ulcerations in the intestinal mucosa, increased number of caliciform cells, greater vascular congestion of the submucosal layer and inflammatory reaction were related to increasing length of time without transit. Key words: Colon. Colitis. Colostomy. Image Processing, Computer-Assisted. Rats. RESUMOObjetivo: Avaliar por método de imagem assistida por computador as alterações histopatológicas da parede cólica em segmentos providos e desprovidos de trânsito intestinal e relacioná-las ao tempo de exclusão. Métodos: Trinta ratos Wistar machos foram submetidos à derivação do trânsito no cólon esquerdo por meio de colostomia proximal e fístula mucosa distal. Os animais foram divididos em três grupos experimentais segundo o sacrifício ter sido realizado seis, doze e dezoito semanas após o procedimento cirúrgico inicial. Segmentos dos cólons providos e desprovidos de trânsito foram submetidos a estudo histopatológico. Foram analisadas as variáveis: comprimento das criptas cólicas, ulceração na mucosa, espessura das camadas muscular da mucosa, submucosa e muscular própria, congestão vascular, número...

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Enhanced translocation of bacteria across metabolically stressed epithelia is reduced by butyrate†

Lewis

Lutgendorff

Phan

et al. 2010

Inflammatory Bowel Diseases

250

146

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Diversion procto-colitis: Response to treatment with short-chain fatty acids

Cited by 47 publications

References 17 publications

Quantification by computerized morphometry of tissue levels of sulfomucins and sialomucins in diversion colitis in rats

Quantification by computerized morphometry of tissue levels of sulfomucins and sialomucins in diversion colitis in rats

Evaluation by computerized morphometry of histopathological alterations of the colon wall in segments with and without intestinal transit in rats

Enhanced translocation of bacteria across metabolically stressed epithelia is reduced by butyrate†

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