Divergent Cryptosporidium parvum subtype and Enterocytozoon bieneusi genotypes in dromedary camels in Algeria

Baroudi, Djamel; Zhang, Hongwei; Amer, Said; Khelef, Djamel; Roellig, Dawn M.; Wang, Yuanfei; Feng, Yaoyu; Xiao, Lihua

doi:10.1007/s00436-017-5734-1

Cited by 25 publications

(24 citation statements)

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“…Of the 12 C. parvum isolates, 5 were identified as subtype IIdA19G1 (n = 4) or subtype IIdA15G1 (n = 1) based on sequencing analysis of the gp60 gene, and these 2 subtypes have also frequently been found in bovines [31]. In addition, subtypes IIdA19G1 and IIdA15G1 have also been detected in ruminants (sheep, goats, deer), alpacas, horses, rodents (mice, hamsters, squirrels), pigs, carnivores (dogs, gray wolves, raccoon dogs), and in wastewater around the world [3,19]. Meanwhile, previous reports have shown that subtypes IIdA15G1 and IIdA19G1 can also cause human infections [13,30].…”

Section: Discussionmentioning

confidence: 99%

Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in dairy cattle in Gansu, northwest China

et al. 2020

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Cryptosporidium spp. and Giardia duodenalis are common gastrointestinal parasites with a broad range of hosts, including humans, livestock, and wildlife. To examine the infection status and assess the zoonotic potential of Cryptosporidium spp. and G. duodenalis in dairy cattle in Gansu, China, a total of 1414 fecal samples were collected from the rectum, with one sample collected from each individual animal. All the samples were tested using nested PCR based on the small subunit ribosomal RNA (SSU rRNA) gene of Cryptosporidium spp. and G. duodenalis. The overall infection rates of Cryptosporidium spp. and Giardia duodenalis were 4.2% (n = 59) and 1.0% (n = 14), respectively. Four Cryptosporidium species were identified: C. andersoni (n = 42), C. parvum (n = 12), C. bovis (n = 5), and C. ryanae (n = 1). In further analyses of subtypes of C. parvum isolates based on the 60 kDa glycoprotein (gp60) gene, five were successfully subtyped as IIdA19G1 (n = 4) and IIdA15G1 (n = 1). All 14 G. duodenalis isolates were identified as assemblage E using the triosephosphate isomerase (tpi) gene. The relatively low positive rates of Cryptosporidium spp. and G. duodenalis detected here and the predominance of non-human pathogenic species/assemblages of these parasites indicated their unique transmission dynamics in this area and the low level of threat posed to public health. However, continuous monitoring and further studies of these parasites should be conducted for the prevention and control of these pathogens.

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Section: Discussionmentioning

confidence: 99%

Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in dairy cattle in Gansu, northwest China

et al. 2020

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“…[ 14 , 15 ]. Notwithstanding, little is available on the gastrointestinal parasitic infections in dromedary camels in Algeria [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Gastrointestinal parasites of dromedary camel (Camelus dromedarius) in Algeria

et al. 2020

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Aim: The present study was designed to investigate the prevalence and identification of gastrointestinal parasites in feces samples of dromedary camels (Camelus dromedarius) in Algeria based on microscopic examination. Materials and Methods: A total of 717 fresh fecal samples obtained from 28 farms at Steppe and Northern Sahara regions of Algeria were processed for microscopic examination after concentration by formalin-ether sedimentation and flotation techniques. In addition, microscopic examination of Cryptosporidium spp. was done by modified Ziehl-Neelsen staining and Lugol staining procedure was used for the detection of Giardia cysts. Results: Microscopic examination indicated an infection rate of gastrointestinal parasites of 48.26% (346/717). Protozoan infections were recorded at 17.02% (122/717), whereas helminth infections were recorded at 23.71% (170/717). In addition, mixed infection (protozoans and helminths) was seen at 7.53% (54/717). No correlation was found between infection and age of the animals, nor the consistency of the stool samples; in addition, neither influence of sex nor breed of camels was observed. Eighteen genera of gastrointestinal parasites were revealed; including four genera of protozoa, 12 Nematoda, one Cestoda, and one Trematoda. Strongyloides spp. and Eimeria spp. showed the highest rate of parasitism, while Cooperia spp. was observed with the lowest prevalence. Cryptosporidium spp. was detected in 13 among 717 examined samples (1.81%). Conclusion: The parasite fauna infecting the gastrointestinal tract of the Algerian dromedary is much diversified. The detected parasites in camels are similar to counterparts in other ruminants, posing serious challenge to animal farming. Future studies should be carried out to better understand the epidemiology of these parasitic diseases and their economic and public health impact.

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“…Although Cryptosporidium spp. from cattle, horses, camels and chickens have been recently characterized using molecular biological tools [ 20 – 23 ], little information is available on the identity of Cryptosporidium spp. in goats and sheep.…”

Section: Introductionmentioning

confidence: 99%

Zoonotic Cryptosporidium species and subtypes in lambs and goat kids in Algeria

et al. 2018

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BackgroundLittle is known on the occurrence and identity of Cryptosporidium species in sheep and goats in Algeria. This study aimed at investigating the occurrence of Cryptosporidium species in lambs and goat kids younger than 4 weeks.MethodsA total of 154 fecal samples (62 from lambs and 92 from kid goats) were collected from 13 sheep flocks in Médea, Algeria and 18 goat flocks across Algiers and Boumerdes. They were screened for Cryptosporidium spp. by nested-PCR analysis of a fragment of the small subunit (SSU) rRNA gene, followed by restriction fragment length polymorphism and sequence analyses to determine the Cryptosporidium species present. Cryptosporidium parvum and C. ubiquitum were further subtyped by sequence analysis of the 60 kDa glycoprotein gene.ResultsCryptosporidium spp. were detected in 17 fecal samples (11.0%): 9 from lambs (14.5%) and 8 from goat kids (8.7%). The species identified included C. parvum in 3 lambs, C. xiaoi in 6 lambs and 6 goat kids, and C. ubiquitum in 2 goat kids. Cryptosporidium infections were detected mostly in animals during the first two weeks of life (7/8 for goat kids and 7/9 for lambs) and in association with diarrhea occurrence (7/17 or 41.2% goat kids and 7/10 or 70.0% lambs with diarrhea were positive for Cryptosporidium spp.). Subtyping of C. parvum and C. ubiquitum isolates identified the zoonotic IIaA13G2R1 and XIIa subtype families, respectively. Minor differences in the SSU rRNA gene sequences were observed between C. xiaoi from sheep and goats.ConclusionsResults of this study indicate that three Cryptosporidium species occur in lambs and goat kids in Algeria, including zoonotic C. parvum and C. ubiquitum. They are associated with the occurrence of neonatal diarrhea.

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Divergent Cryptosporidium parvum subtype and Enterocytozoon bieneusi genotypes in dromedary camels in Algeria

Cited by 25 publications

References 24 publications

Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in dairy cattle in Gansu, northwest China

Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in dairy cattle in Gansu, northwest China

Gastrointestinal parasites of dromedary camel (Camelus dromedarius) in Algeria

Zoonotic Cryptosporidium species and subtypes in lambs and goat kids in Algeria

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