Dithio-Phospholipids for Biospecific Immobilization of Proteins on Gold Surfaces

Techniques for phosphopeptide enrichment prior to analysis by mass spectrometry

“…A number of research groups have prepared custom MALDI plates with IMAC functionalities. The immobilization of SAMs derivatized with NTA analogs was first reported in 1996, and a number of subsequent studies demonstrated the fabrication of similar monolayer films (Sigal et al, 1996; Stora et al, 1997; Scheibler et al, 1998; Kada et al, 2002; Luk et al, 2003; Makower et al, 2003; Rigler et al, 2003; Wegner et al, 2003; Gamsjaeger et al, 2004; Lee et al, 2004; Rigler, Ulrich, & Vogel, 2004; Trammell et al, 2004; Maly et al, 2004a,b; Johnson & Martin, 2005; Tinazli et al, 2005; Klenkar et al, 2006; Valiokas et al, 2006; Ataka & Heberle, 2006; Ataka, Richter, & Heberle, 2006). These NTA SAMs have been employed in the study of histidine–nickel, protein–protein, protein–antibody, protein–DNA, or protein–ligand interactions, and for biotechnology applications including microarrays, biosensors, catalysis, and biocompatible coatings.…”

Techniques for phosphopeptide enrichment prior to analysis by mass spectrometry

“…A number of research groups have prepared custom MALDI plates with IMAC functionalities. The immobilization of SAMs derivatized with NTA analogs was first reported in 1996, and a number of subsequent studies demonstrated the fabrication of similar monolayer films (Sigal et al, 1996; Stora et al, 1997; Scheibler et al, 1998; Kada et al, 2002; Luk et al, 2003; Makower et al, 2003; Rigler et al, 2003; Wegner et al, 2003; Gamsjaeger et al, 2004; Lee et al, 2004; Rigler, Ulrich, & Vogel, 2004; Trammell et al, 2004; Maly et al, 2004a,b; Johnson & Martin, 2005; Tinazli et al, 2005; Klenkar et al, 2006; Valiokas et al, 2006; Ataka & Heberle, 2006; Ataka, Richter, & Heberle, 2006). These NTA SAMs have been employed in the study of histidine–nickel, protein–protein, protein–antibody, protein–DNA, or protein–ligand interactions, and for biotechnology applications including microarrays, biosensors, catalysis, and biocompatible coatings.…”

How to Catch a Membrane Protein in Action: A Review of Functional Membrane Protein Immobilization Strategies and Their Applications

“…Specific interactions between the protein or membrane and the surface have been used in place of random ones for better control of the protein’s orientation. The most common strategies involve the biotin interaction with streptavidin or avidin. ,− In one case, a biotinylated Neurokinin-1 receptor was overexpressed in CHO cell lines and immobilized through a streptavidin linker to a biotin covered slide . The procedure was very simple, consisting primarily of treating the quartz slides with biotinylated bovine serum albumin (BSA), which results in a surface resistant to salt and detergent washes.…”

Controlled Formation and Mixing of Two-Dimensional Fluids

“…Furthermore, lipid monolayers could be formed by the traditional Langmiur−Blodget deposition technique or by lipid vesicle adsorption. , Self-assembled monolayers (SAMs) represent another class of molecularly thin films that, in contrast to lipid or surfactant systems, do not possess properties of a fluid. SAMs, which typically are composed of alkanthiolates or other alkyl compounds on Au, Ag, and Cu are easily formed and have been shown to be very useful for surface modification and functionalization on the micrometer scale. − …”

“…SAMs, which typically are composed of alkanthiolates or other alkyl compounds on Au, Ag, and Cu 10 are easily formed 11 and have been shown to be very useful for surface modification and functionalization on the micrometer scale. [12][13][14][15] We have developed a concept for controlled formation of liquid films on microfabricated hydrophobic substrates that we call dynamic liquid film formation (DLFF). In contrast to previous methods of fabrication, this method allows for stoichiometric control of the different components included in the film.…”

Techniques for phosphopeptide enrichment prior to analysis by mass spectrometry

“…A number of research groups have prepared custom MALDI plates with IMAC functionalities. The immobilization of SAMs derivatized with NTA analogs was first reported in 1996, and a number of subsequent studies demonstrated the fabrication of similar monolayer films (Sigal et al, 1996; Stora et al, 1997; Scheibler et al, 1998; Kada et al, 2002; Luk et al, 2003; Makower et al, 2003; Rigler et al, 2003; Wegner et al, 2003; Gamsjaeger et al, 2004; Lee et al, 2004; Rigler, Ulrich, & Vogel, 2004; Trammell et al, 2004; Maly et al, 2004a,b; Johnson & Martin, 2005; Tinazli et al, 2005; Klenkar et al, 2006; Valiokas et al, 2006; Ataka & Heberle, 2006; Ataka, Richter, & Heberle, 2006). These NTA SAMs have been employed in the study of histidine–nickel, protein–protein, protein–antibody, protein–DNA, or protein–ligand interactions, and for biotechnology applications including microarrays, biosensors, catalysis, and biocompatible coatings.…”

How to Catch a Membrane Protein in Action: A Review of Functional Membrane Protein Immobilization Strategies and Their Applications

“…Specific interactions between the protein or membrane and the surface have been used in place of random ones for better control of the protein’s orientation. The most common strategies involve the biotin interaction with streptavidin or avidin. ,− In one case, a biotinylated Neurokinin-1 receptor was overexpressed in CHO cell lines and immobilized through a streptavidin linker to a biotin covered slide . The procedure was very simple, consisting primarily of treating the quartz slides with biotinylated bovine serum albumin (BSA), which results in a surface resistant to salt and detergent washes.…”

Controlled Formation and Mixing of Two-Dimensional Fluids

“…Furthermore, lipid monolayers could be formed by the traditional Langmiur−Blodget deposition technique or by lipid vesicle adsorption. , Self-assembled monolayers (SAMs) represent another class of molecularly thin films that, in contrast to lipid or surfactant systems, do not possess properties of a fluid. SAMs, which typically are composed of alkanthiolates or other alkyl compounds on Au, Ag, and Cu are easily formed and have been shown to be very useful for surface modification and functionalization on the micrometer scale. − …”

“…SAMs, which typically are composed of alkanthiolates or other alkyl compounds on Au, Ag, and Cu 10 are easily formed 11 and have been shown to be very useful for surface modification and functionalization on the micrometer scale. [12][13][14][15] We have developed a concept for controlled formation of liquid films on microfabricated hydrophobic substrates that we call dynamic liquid film formation (DLFF). In contrast to previous methods of fabrication, this method allows for stoichiometric control of the different components included in the film.…”

Techniques for phosphopeptide enrichment prior to analysis by mass spectrometry

“…A number of research groups have prepared custom MALDI plates with IMAC functionalities. The immobilization of SAMs derivatized with NTA analogs was first reported in 1996, and a number of subsequent studies demonstrated the fabrication of similar monolayer films (Sigal et al, 1996; Stora et al, 1997; Scheibler et al, 1998; Kada et al, 2002; Luk et al, 2003; Makower et al, 2003; Rigler et al, 2003; Wegner et al, 2003; Gamsjaeger et al, 2004; Lee et al, 2004; Rigler, Ulrich, & Vogel, 2004; Trammell et al, 2004; Maly et al, 2004a,b; Johnson & Martin, 2005; Tinazli et al, 2005; Klenkar et al, 2006; Valiokas et al, 2006; Ataka & Heberle, 2006; Ataka, Richter, & Heberle, 2006). These NTA SAMs have been employed in the study of histidine–nickel, protein–protein, protein–antibody, protein–DNA, or protein–ligand interactions, and for biotechnology applications including microarrays, biosensors, catalysis, and biocompatible coatings.…”

How to Catch a Membrane Protein in Action: A Review of Functional Membrane Protein Immobilization Strategies and Their Applications

“…Specific interactions between the protein or membrane and the surface have been used in place of random ones for better control of the protein’s orientation. The most common strategies involve the biotin interaction with streptavidin or avidin. ,− In one case, a biotinylated Neurokinin-1 receptor was overexpressed in CHO cell lines and immobilized through a streptavidin linker to a biotin covered slide . The procedure was very simple, consisting primarily of treating the quartz slides with biotinylated bovine serum albumin (BSA), which results in a surface resistant to salt and detergent washes.…”

Controlled Formation and Mixing of Two-Dimensional Fluids

“…Furthermore, lipid monolayers could be formed by the traditional Langmiur−Blodget deposition technique or by lipid vesicle adsorption. , Self-assembled monolayers (SAMs) represent another class of molecularly thin films that, in contrast to lipid or surfactant systems, do not possess properties of a fluid. SAMs, which typically are composed of alkanthiolates or other alkyl compounds on Au, Ag, and Cu are easily formed and have been shown to be very useful for surface modification and functionalization on the micrometer scale. − …”

“…SAMs, which typically are composed of alkanthiolates or other alkyl compounds on Au, Ag, and Cu 10 are easily formed 11 and have been shown to be very useful for surface modification and functionalization on the micrometer scale. [12][13][14][15] We have developed a concept for controlled formation of liquid films on microfabricated hydrophobic substrates that we call dynamic liquid film formation (DLFF). In contrast to previous methods of fabrication, this method allows for stoichiometric control of the different components included in the film.…”