Distributions of HLA‐DRB1/DQB1 alleles and haplotypes in the North‐eastern Thai population: indicative of a distinct Thai population with Chinese admixtures in the Central Thais

Abstract

The phenotype and gene frequencies of HLA class II alleles were studied in the North‐eastern Thai population. Blood samples were collected from 100 unrelated healthy North‐eastern Thais. The HLA‐DRB1 and DQB1 genes were typed using the polymerase chain reaction – sequence specific primer (PCR‐SSP) and polymerase chain reaction – sequence specific oligonucleotide probe (PCR‐SSO) techniques. Twenty‐six HLA‐DRB1 and 11 DQB1 alleles were found in this population. DRB1*1202, 1502, 0405 and DQB1*0502/0504, 0301/0304 alleles were commonly found. Linkage disequilibrium analysis suggested the existence of 13 DR‐DQ haplotypes. The DRB1*1502‐DQB1*0501 haplotype was the most common. The DRB1*1106‐DQB1*0301/0304 haplotype was found only in North‐eastern Thais and not in other Thai populations. Comparative analysis of the HLA‐DR/DQ alleles revealed differences in the distributions of these alleles amongst different ethnic groups. Interestingly, the distributions of HLA class II alleles in Central Thai, North‐eastern Thai and Southern Chinese populations are similar. However, it appears that the distribution in the Central Thais is a mixture of those in Southern Chinese and North‐eastern Thais, suggesting the existence of Thai–Chinese admixtures in the Central Thai population. This study provides basic information for further studies of the MHC in anthropology, organ transplantation and disease susceptibility in the North‐eastern Thai population.

HLA‐B*15 subtypes in the population of north‐eastern Thailand

“…Table 4 summarizes the possible association of HLA‐B*15 alleles with other HLA alleles. The data for other loci are from a previous study (Romphruk et al. , manuscript in preparation).…”

“…A total of 80 HLA‐B15 positive samples were obtained from 300 unrelated healthy individuals from north‐eastern Thailand (Romphruk et al. , manuscript in preparation).…”

“…Six groups of B*15 could be defined by this protocol. Four groups of B*15 were identified in this population: B*15 (01, 04–07, 12, 14, 19, 20, 24, 25, 26N, 27 and 32–35) [allele frequency (AF) = 3.67%], B*15 (02, 08, 11, 15, 28 and 30) (AF = 8.67%), B*1503/4802 (AF = 0.5%), and B*1521 (AF = 0.5%) (Romphruk et al. , manuscript in preparation).…”

Human Papillomavirus Genotypes and Cervical Cancer in Northeast Thailand

“…Certain HLA haplotypes alter disease susceptibilities vis-à-vis the relationships between HTLV-1 infection and ATL and/or HAM (Sonoda, 1990) and EBV and NPC (Goldsmith et al, 2002), which were well documented viral oncology issues. Genetic composition of the Thai population has Chinese contributions and HLA-DQB1*06 is common especially among northeastern Thais (Romphruk et al, 1999;Romphruk et al, 2010). If the HLA type has an increasing effect on SCCA development, a rather high prevalence of HPV-58 in SCCA patients in northeastern Thailand would be consistent.…”

Burkholderia pseudomalleiProteins Presented by Monocyte-Derived Dendritic Cells Stimulate Human Memory T CellsIn Vitro

“…HLA-DRB1 alleles in the samples used above were determined by PCR sequence-specific primer (PCR-SSP), using 20 pairs of primers (22,23). The primer mixtures contained a pair of allele-specific primers and a pair of 256-bp internal control primers.…”