Distribution of the calcium binding proteins, calbindin D-28K and parvalbumin, in the subicular complex of the adult mouse

Fujise, Noboru; Hunziker, Willi; Heizmann, Claus W.; Kosaka, Toshio

doi:10.1016/0168-0102(95)00882-t

Cited by 26 publications

(27 citation statements)

References 28 publications

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“…Although this term has been applied to rodents (Lorente de Nó, 1934), it is used most often in studies of primates (Rosene and Van Hoesen, 1987;Barbas and Blatt, 1995). There is evidence to support the separation of the prosubiculum and subiculum based on distinct layering patterns (Lorente de Nó, 1934) connections (Barbas and Blatt, 1995) and chemistry (Fujise et al, 1995). However, in our study in rat, we have decided to use the description ''the proximal part of the subiculum'' for the area adjacent to CA1, because this avoids relying on comparisons with studies in other species or studies that used different markers to delineate the region.…”

Section: Discussionmentioning

confidence: 95%

“…Pyramidal neurons in this part of the subiculum of mouse have been shown to be immunoreactive for calbindin (Fujise et al, 1995). A study in rat also revealed a similar population of calbindin-immunoreactive pyramidal neurons (Sloviter, 1989), although this is not always reported (Seress et al, 1993(Seress et al, , 1994.…”

Section: Distribution Of Nnos-immunopositive Neuronsmentioning

confidence: 89%

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Light and electron microscopic study of neuronal nitric oxide synthase-immunoreactive neurons in the rat subiculum

Lin¹,

Totterdell²

1998

J. Comp. Neurol.

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Neurons in the rat subiculum that are capable of producing nitric oxide were studied by using an antibody to the neuronal isoform of nitric oxide synthase (nNOS). In the light microscope, the staining pattern with the nNOS antibody closely resembled that seen following histochemical processing with nicotinamide adenine dinucleotide phosphate diaphorase. Immunostained neurons were found in all layers, and, in addition, large dendrites in the apical dendrite layer were also immunopositive. Although a few immunolabelled cells had the typical morphology of interneurons, most were found to have the characteristics of pyramidal neurons. In the subiculum, these immunoreactive pyramidal neurons were concentrated mainly in the most superficial cell layers and closest to the CA1 region, but pyramidal neurons in the CA1 layer of the hippocampus were consistently immunonegative. Immunopositive profiles in the subiculum were studied in the electron microscope and compared with unlabelled structures. Ultrastructural criteria suggest that both pyramidal and nonpyramidal subicular neurons are immunopositive for nNOS. Large, spiny dendrites and smaller, varicose dendrites were found to be immunoreactive for nNOS. Vesicle-containing profiles were probably presynaptic axons, and immunopositive boutons were seen to make symmetrical and asymmetrical synaptic contacts.

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Section: Discussionmentioning

confidence: 95%

Section: Distribution Of Nnos-immunopositive Neuronsmentioning

confidence: 89%

Light and electron microscopic study of neuronal nitric oxide synthase-immunoreactive neurons in the rat subiculum

Lin¹,

Totterdell²

1998

J. Comp. Neurol.

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“…In the rat and other rodents, the parasubiculum is an area with very distinctive cytoarchitecture, neurochemistry, and connectivity (cf. Stephan, 1975;van Groen and Wyss, 1990;Fujise et al, 1995). In humans, the existence of a parasubiculum has been questioned (for an extensive discussion of earlier literature, see Stephan, 1975, pp.…”

Section: Neuron Numbers and Hippocampal Connectivitymentioning

confidence: 98%

Neuron numbers in the presubiculum, parasubiculum, and entorhinal area of the rat

1997

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Estimates of neuron numbers have been useful in studies of neurodegenerative disorders, and in their animal models, and in the computational modeling of hippocampal function. Although the retrohippocampal region (presubiculum, parasubiculum, and entorhinal area) is an integral part of the hippocampal circuitry and is affected selectively in a number of disorders, estimates of neuron numbers in the rat retrohippocampal region have yet to be published. Such data are necessary ingredients for computational models of the function of this region and will also facilitate a comparison of this region in rats and primates, which will help to determine how well we may expect rat models to predict function and dysfunction in primate brains. In the present study, we used the optical fractionator to estimate the number of neurons in the rat retrohippocampal region. The following estimates were obtained: 3.3 x 10(5) in presubicular layers II and III, 1.5 x 10(5) in parasubicular layers II and III, 2.2 x 10(5) in the combined pre- and parasubicular layers V and VI, 6.6 x 10(4) in medial entorhinal area (MEA) layer II, 1.3 x 10(5) in MEA layer III, 1.9 x 10(5) in MEA layers V and VI, 4.6 x 10(4) in lateral entorhinal area (LEA) layer II, 1.2 x 10(5) in LEA layer III, and 1.4 x 10(5) in LEA layers V and VI. A surprising finding was the large numbers of neurons in the pre- and parasubiculum, which indicate an important role of these areas in the control of the entorhino-hippocampal projection. A comparison of the numbers of neurons in the hippocampus and entorhinal areas in rats with similar estimates in humans revealed that gross input-output relations are largely conserved. Differences between rats and humans may be accounted for by more prominent entorhino-neocortical projections in primates and consequent increases in the number of neurons in the hippocampus and retrohippocampal region, which are dedicated to these projections.

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“…However, cytoarchitectonically and histochemically the borders are relatively easy to distinguish, i.e., in Nissl-stained material the border is characterized by an abrupt change in the size and staining density of layer II neurons (they are larger and darker in the entorhinal cortex). In parvalbumin-stained material the border between EC and PRh is characterized by a sudden change in staining intensity, i.e., whereas the EC is darkly stained, the PRh is very lightly stained (e.g., Celio, 1990;Tuñon et al, 1992;Fujise et al, 1995;Wouterlood et al, 1995;Insausti et al, 1997). Further, the entorhinal cortex is lightly stained in calbindin-stained material, whereas the parasubicular cortex is densely stained (Fujise et al, 1995;Fujimaru and Kosaka, 1996).…”

mentioning

confidence: 97%

Entorhinal cortex of the mouse: Cytoarchitectonical organization

Groen

2001

Hippocampus

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The present study describes the cytoarchitectonical and chemoarchitectonical organization of the entorhinal cortex of the mouse (C57BL/6J strain). The entorhinal cortex is medially bordered by the parasubiculum, and laterally by the perirhinal cortex; rostrally and medially it is bordered by the piriform cortex, whereas caudally and dorsally it is bordered by the postrhinal cortex. The entorhinal cortex is divided into two main areas, i.e., the lateral entorhinal area (LEA) and the medial entorhinal area (MEA). Both entorhinal areas are further divided into subfields, i.e., LEA is divided into DLE (dorsolateral entorhinal field), DIE (dorsal intermediate entorhinal field), and VIE (ventral intermediate entorhinal field), whereas MEA is divided into CE (caudal entorhinal field) and ME (medial entorhinal field). Cytoarchitectonically, the main difference between LEA and MEA is displayed by layer II neurons: while these are in a dense layer in LEA, they are more dispersed in MEA. Further, in LEA there is a relatively cell-free zone between layers II and III; this zone is not present in MEA. Histochemically, in acetylcholinesterase (AChE)-stained material, MEA is characterized by darker-stained bands in the superficial layer (i.e., layer I) and in the lamina dissecans, in contrast to LEA, which is more evenly stained for AChE. Further, both the border with the perirhinal cortex and the border with the parasubiculum are characterized by dark-stained bands of AChE. The border between the entorhinal cortex and perirhinal cortex is also easily distinguished in parvalbumin-stained material; while the entorhinal cortex is darkly stained, the perirhinal cortex is lightly stained. In contrast, in sections stained for calretinin, the entorhinal cortex is more lightly stained than the parasubiculum, which has a darkly stained superficial layer, and a densely stained group of neurons in layer III.

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Distribution of the calcium binding proteins, calbindin D-28K and parvalbumin, in the subicular complex of the adult mouse

Cited by 26 publications

References 28 publications

Light and electron microscopic study of neuronal nitric oxide synthase-immunoreactive neurons in the rat subiculum

Light and electron microscopic study of neuronal nitric oxide synthase-immunoreactive neurons in the rat subiculum

Neuron numbers in the presubiculum, parasubiculum, and entorhinal area of the rat

Entorhinal cortex of the mouse: Cytoarchitectonical organization

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