2005
DOI: 10.1590/s0100-879x2005000300009
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Abstract: The expression of components present in the cartilaginous extracellular matrix is related to development, gender, and genotype, as well as to the biomechanical properties of each type of cartilage. In the present study, we analyzed small proteoglycans and glycosaminoglycans present in different cartilages of the chicken wing after extraction with guanidine hydrochloride or papain. Quantitative analysis of glycosaminoglycans showed a larger amount in humeral cartilage (around 200 mg/g tissue) than in articular … Show more

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Cited by 5 publications
(4 citation statements)
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References 32 publications
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“…It would therefore be expected that the relative amount of digestion would be highest in the superficial zone with progressively less towards the deeper zones of the tissue. However, it is also important to take into account the fact that the SLRPs decorin and biglycan, with their GAG chains, are found in relatively high concentrations within the superficial and transitional zones (Poole et al, 1996;Rodrigues et al, 2005). As expected, digestion within the superficial zone was more extensive than within other zones of the cartilage.…”
Section: The Effect O F Concentration and Incubation Time O F Chondromentioning
confidence: 81%
“…It would therefore be expected that the relative amount of digestion would be highest in the superficial zone with progressively less towards the deeper zones of the tissue. However, it is also important to take into account the fact that the SLRPs decorin and biglycan, with their GAG chains, are found in relatively high concentrations within the superficial and transitional zones (Poole et al, 1996;Rodrigues et al, 2005). As expected, digestion within the superficial zone was more extensive than within other zones of the cartilage.…”
Section: The Effect O F Concentration and Incubation Time O F Chondromentioning
confidence: 81%
“…The mass ratios of CSC/DS conjugated to the scaffolds were evaluated indirectly by quantifying the hydrolyzed residues of CSC (DDi-6S) and DS (DDi-4S) in the waste PBS using SAX-HPLC [7,17] with some modifications. Briefly, the waste GAGs solutions for each reaction were collected, lyophilized and then dissolved in 0.9 ml Tris-acetate buffer (50 mM, pH 8.0).…”
Section: Characterization Of Scaffoldsmentioning
confidence: 99%
“…Aside from the bulk cross-linking efficiency, we further evaluated whether CSC and DS were cross-linked to the chitosan scaffolds to the same degree. Since the disaccharide units of DS (DDi-4S) and CSC (DDi-6S) consist of 4-sulfated and 6-sulfated N-acetylgalactosamine, respectively, the D-disaccharide compositions of the chondroitin/DS chains in the native or engineered tissues can be characterized after enzyme digestion by fluorophore-assisted carbohydrate electrophoresis [22] or HPLC [7,17]. However, the cross-linking reactions during scaffold preparation could lead to structural changes of DS and CSC in the side chains, resulting in the loss of functional groups for enzyme digestion and incorrect quantification.…”
Section: Article In Pressmentioning
confidence: 99%
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