Distribution of Secondary Metabolites in Brassica Napus Genotypes

Abstract

Secondary metabolites (flavonoids and phenolics) in the methanolic extracts of Brassica napus genotypes, spring and winter canola were evaluated. Antioxidant activity of the extracts was evaluated by b-carotene linoleic acid model system, scavenging ability of hydrogen peroxide (H 2 O 2 ) assay and cupric reducing antioxidant capacity (CUPRAC) assay. Also, total flavonoid contents of the extracts were determined by aluminum chelating method. Results showed that naringin and eriodictyol were found to be in both species. Data showed that winter canola exihibited high antioxidant capacity with 60.6 Ϯ 0.4% in b-carotene linoleic acid model system and also CUPRAC value (4.18 Ϯ 0.11 mM TEAC/g) and scavenging ability of H 2 O 2 (57.12 Ϯ 1.13%) was higher than spring canola. However, total flavonoid content of spring canola was higher than that of winter canola. Both canola species may be of importance in variety improvement, nutraceuticals, bio-pharmaceuticals and food additives as natural antioxidants. PRACTICAL APPLICATIONSThis study reveals antioxidant activity of the methanolic extracts of Brassica napus genotypes, spring and winter canola seeds growing in Turkey, measured by various assays including by b-carotene linoleic acid model system, scavenging ability of hydrogen peroxide assay and cupric reducing antioxidant capacity (CUPRAC) assay. Total flavonoid contents of the extracts were also measured as quercetin equivalent by aluminum chelating method. Secondary metabolites of the extracts of Brassica napus genotypes were analyzed by high-performance liquid chromatography.

Determination of antimicrobial effect, antioxidant activity and phenolic contents of desert truffle in Turkey

“…The phenolic substances were determined as described by Maltas & Yildiz (2011). Gallic acid, catechin, caffeic acid, p-coumaric acid, ferulic acid, cinnamic acid and quercetin (Sigma-Aldrich) were analysed as standard phenolic structures.…”

Optimization of Ultrasound-Assisted Extraction Procedure to Determine Antioxidant Capacity of Rapeseed Cultivars

“…The total content of polar phenolics in rapeseed is significantly higher (about ten times) than those of insoluble components. Therefore, more polar solvent such as methanol and their aqueous mixture are usually used for extraction of phenolics from rapeseed (Amarowicz et al 2003;Dwiecki et al 2012;Farag et al 2013;Khattab et al 2010;Maltas and Yildiz 2011;Matthäus 2002;Szydłowska-Czerniak et al 2010, 2013. Antioxidants present in rapeseed and its products are important in the prevention and treatment of coronary heart diseases, neurodegenerative and autoimmune diseases, aging, cancers, diabetes, hypertension, and rheumatoid arthritis and exhibit antiradical activity (Szydłowska-Czerniak 2013).…”

“…Only few methods, 2,2′-diphenyl-1-picrylhydrazyl (DPPH) (Amarowicz et al 2000(Amarowicz et al , 2003Dwiecki et al 2012;Farag et al 2013;Khattab et al 2010;Matthäus 2002;Szydłowska-Czerniak et al 2010Szydłowska-Czerniak and Tułodziecka 2013;Yoshie-Stark et al 2006), ferric-reducing antioxidant power (FRAP) (Szydłowska-Czerniak et al 2010Szydłowska-Czerniak and Tułodziecka 2013), reducing power (Amarowicz et al 2000(Amarowicz et al , 2003, oxygen radical absorbance capacity (ORAC) (Szydłowska-Czerniak et al 2010), cupric reducing antioxidant capacity (CUPRAC) (Maltas and Yildiz 2011), β-carotene-linoleic acid (Amarowicz et al 2003;Maltas and Yildiz 2011;Matthäus 2002), scavenging ability of hydrogen peroxide (Maltas and Yildiz 2011), electron spin resonance (ESR) (Matthäus 2002), enhanced chemiluminescence (ECL) (Amarowicz et al 2003), photochemiluminescence (PCL) (Amarowicz et al 2003), and assay based on formation of silver nanoparticles (AgNP) (Szydłowska-Czerniak and Tułodziecka 2013), were used for determination of antioxidant capacity of different rapeseed varieties.…”

“…Different traditional methods such as boiling, heating, conventional solvent, and Soxhlet extractions have been used to extract antioxidant compounds from rapeseed samples (Amarowicz et al 2003;Dwiecki et al 2012;Maltas and Yildiz 2011;Szydłowska-Czerniak and Tułodziecka 2013). These procedures of sample preparation include the consumption of large amount of solvent, long extraction time, loss of phenols due to oxidation, hydrolysis, and ionization during extraction.…”

Comparison of winter and summer canola (Brassica napus) genotypes in Turkey

“…Our group has studied the biochemical characteristics of 2 B. napus (canola) genotypes, in Turkey, cultivated in winter and spring (Maltas and Yildiz, 2011). In this study, we characterized the genetic diversity and determined the pedigree relationships between both genotypes of B. napus using RAPD and the OPA1-15 primers.…”