Distribution of olive oil phenolic compounds in rat tissues after administration of a phenolic extract from olive cake

Abstract

After a single ingestion of olive oil phenolic compounds, these were absorbed, metabolized and distributed through the blood stream to practically all parts of the body, even across the blood-brain barrier.

Olive Leaf Extracts Are a Natural Source of Advanced Glycation End Product Inhibitors

“…It has been shown that after an acute ingestion of olive phenolic compounds, they are absorbed, metabolized, and distributed though the blood stream to practically all parts of the body, even across the blood-brain barrier. 24 Free forms of certain phenolic compounds were determined in certain tissues, that is, oleuropein in the plasma and brain, luteolin in the kidney, testicle, brain, and heart, or hydroxytyrosol in the plasma, kidney, and testicle, but plasma mainly contained the metabolites. Using the Caco-2/TC7 cells as a model of the human intestinal epithelium, limited metabolism of olive oil phenolics was observed; the methylated conjugates were the major metabolites detected.…”

Oleuropein as an inhibitor of peroxisome proliferator-activated receptor gamma

“…Recent studies based on metabolite-profiling approaches demonstrated that oleuropein is rapidly absorbed and distributed through the blood stream to various tissues in vivo (Serra et al 2012) and entered to cell cytoplasm in vitro (Quirantes-Pine et al 2013). In acute toxicity studies for oleuropein, no adverse effects were observed in mice at doses as high as 1 g/kg of body weight (Petkov and Manolov 1972).…”

“…In acute toxicity studies for oleuropein, no adverse effects were observed in mice at doses as high as 1 g/kg of body weight (Petkov and Manolov 1972). Taking into account concentration at which PPARc inhibition was detected in vitro (200 lM) and reported level of oleuropein (C max 4 h: 24 lM) in rat plasma (Serra et al 2012) after an acute intake of a phenolic extract from olive cake (3 g/kg body weight, oleuropein content *0.1 mg/g of extract), oleuropein could potentially modulate PPARc activity also in vivo, which needs to be demonstrated.…”

Oleuropein aglycone protects against pyroglutamylated-3 amyloid-ß toxicity: biochemical, epigenetic and functional correlates

“…We used a previously validated technique (Serra et al, 2012), specifically looking for OLE and its main metabolites, hydroxytyrosol (HT), and homovanillic acid (HVA). The mice were sacrificed by cervical dislocation, and the brains were rapidly removed and snap-frozen.…”

“…3.7. Brain concentrations of OLE, HT, and HVA after an acute administration of OLE in wt mice It is known that intestinal OLE is rapidly adsorbed in the plasma of both rats and humans, distributed to various tissues, including brain, conjugated and metabolized in a number of compounds including HT, HVA and elenolic acid, and excreted in the urine (De Bock et al, 2013;Serra et al, 2012). We therefore sought to quantify by liquid chromatographyemass spectrometry/mass spectrometry (LC-MS/MS) the concentrations of OLE and its major metabolites HT and HVA in the brains of OLE-administered mice.…”