Distribution of Myocilin and Extracellular Matrix Components in the Corneoscleral Meshwork of Human Eyes

Ueda, Jun; Yue, Beatrice Y.J.T.

doi:10.1167/iovs.02-1002

Cited by 125 publications

(137 citation statements)

References 34 publications

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“…At the ultrastructural level, decorin and versican immunostaining was found within the electron dense elastic fiber cores and the surrounding microfibril sheath material, both within the TM beams and the JCT region. (Ueda et al 2002;Ueda and Yue 2003) Both proteoglycans were also localized to the long-spaced (100-120 nm periodicity) collagen and the normal collagen fibrils.…”

Section: Proteoglycansmentioning

confidence: 99%

“…(Murphy et al 1987;Marshall et al 1990;Hernandez and Gong 1996;Yue 1996;Zhou et al 1998a;Brilakis et al 2001;Ueda et al 2002;Ueda and Yue 2003;Fuchshofer et al 2006) Interestingly, these components are not strictly limited to the basal lamina of the JCT but are also found dispersed throughout the JCT, perhaps indicating something novel about the origins of the JCT ECM. Laminins have a cruciform shape composed of a heterotrimer of different isoforms of α, β and γ subunits.…”

Section: Basement Membranementioning

confidence: 99%

“…(Floyd et al 1985;Murphy et al 1987;Zhou et al 1998a;Bachmann et al 2006;Keller et al 2007a) In the JCT at the ultrastructural level, fibronectin is found in the amorphous material associated with the basement membranes and with the peripheral SD material of the elastic microfibrils. (Yue 1996;Brilakis et al 2001;Ueda et al 2002;Ueda and Yue 2003) In addition to forming a thin fibrous array that appears to encircle TM cells in culture, (Zhou et al 1998b) fibronectin also forms thick fibrous strands. (Keller et al 2007a) Fibronectin fibril formation has been studied in other tissues and involves interactions with cell surface integrins, proteoglycans and tenascin C. It produces a fibril with considerable elasticity.…”

Section: Fibronectin and Vitronectinmentioning

confidence: 99%

“…(Vittal et al 2005) Vitronectin has also been identified in the TM, associating with the cores and SD material of the elastic microfibrils. (Yue 1996;Ueda et al 2002;Ueda and Yue 2003) Attachment of TM cells to vitronectin also has effects on cell properties. (Yue 1996) …”

Section: Fibronectin and Vitronectinmentioning

confidence: 99%

“…They have been observed associated with basement membranes in areas where collagen or elastic fibrils intersect or link to them; associated with the striated collagen fibrils forming a fine filamentous lattice around and between fibrils; associated with the SD microfibrils and to a lesser extent with the dense elastic cores; and associated with long-spaced collagen. (Lütjen-Drecoll et al 1989;Marshall et al 1991;Hernandez and Gong 1996;Lutjen-Drecoll 1999;Ueda et al 2002;Ueda and Yue 2003) Some variations in their precise localizations were observed, probably reflecting differential epitope availability.…”

Section: Collagensmentioning

confidence: 99%

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Extracellular matrix in the trabecular meshwork

Acott

Kelley

2008

Experimental Eye Research

417

443

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The extracellular matrix (ECM) of the trabecular meshwork (TM) is thought to be important in regulating intraocular pressure (IOP) in both normal and glaucomatous eyes. IOP is regulated primarily by a fluid resistance to aqueous humor outflow. However, neither the exact site nor the identity of the normal resistance to aqueous humor outflow has been established. Whether the site and nature of the increased outflow resistance, which is associated with open-angle glaucoma, is the same or different from the normal resistance is also unclear.The ECMs of the TM beams, juxtacanalicular region (JCT) and Schlemm's canal (SC) inner wall are comprised of fibrillar and non-fibrillar collagens, elastin-containing microfibrils, matricellular and structural organizing proteins, glycosaminoglycans (GAGs) and proteoglycans. Both basement membranes and stromal ECM are present in the TM beams and JCT region. Cell adhesion proteins, cell surface ECM receptors and associated binding proteins are also present in the beams, JCT and SC inner wall region.The outflow pathway ECM is relatively dynamic, undergoing constant turnover and remodeling. Regulated changes in enzymes responsible for ECM degradation and biosynthetic replacement are observed. IOP homeostasis, triggered by pressure changes or mechanical stretching of the TM, appears to involve ECM turnover. Several cytokines, growth factors and drugs, which affect the outflow resistance, change ECM component expression, mRNA alternative splicing, cellular cytoskeletal organization or all of these. Changes in ECM associated with open-angle glaucoma have been identified.

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Section: Proteoglycansmentioning

confidence: 99%

Section: Basement Membranementioning

confidence: 99%

Section: Fibronectin and Vitronectinmentioning

confidence: 99%

Section: Fibronectin and Vitronectinmentioning

confidence: 99%

Section: Collagensmentioning

confidence: 99%

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Extracellular matrix in the trabecular meshwork

Acott

Kelley

2008

Experimental Eye Research

417

443

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Genetic Etiologies of Glaucoma

Wiggs¹

2007

Arch Ophthalmol

180

116

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laucoma can be inherited as a mendelian autosomal-dominant or autosomalrecessive trait, or as a complex multifactorial trait. Genetic approaches have helped define the underlying molecular events responsible for some mendelian forms of the disease and have identified the chromosome locations of genes that are likely to contribute to common complex forms. Future directions include the discovery of new glaucoma genes, determining the clinical phenotypes associated with specific genes and mutations, investigating environmental factors that may contribute to the disease, investigating gene-environment interactions and gene-gene interactions, and developing a mutation database that can be used for diagnostic and prognostic testing.

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Effect of Topical Ginkgo biloba Extract on Steroid-Induced Changes in the Trabecular Meshwork and Intraocular Pressure

Jia

Sun²,

Fan³

et al. 2008

Arch Ophthalmol

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To study the effects of Ginkgo biloba extract (GBE) on dexamethasone (DEX)-induced ocular hypertension. Methods: Rabbits aged 7 weeks received topical Tobra-DEX (Alcon Labs, Hünenberg, Switzerland) and/or 5 µg of GBE four times daily for 14 days. Intraocular pressure (IOP) was recorded every 3 days. After enucleation, trabecular meshwork (TM) cellularity and extracellular matrix deposition were graded. The effect of GBE on apoptosis and expression of myocilin and cell stressrelated genes in DEX-treated human TM cells were studied by immunofluorescence, Western blotting, and quantitative polymerase chain reaction. Results: Ginkgo biloba extract suppressed DEXinduced IOP elevation in rabbits. It reduced the DEXassociated accumulation of extracellular materials within the cribriform layers of the TM and achieved better TM cellularity. In cultured human TM cells, GBE substantially attenuated anti-Fas ligand-induced apoptosis and reduced DEX-induced myocilin expression. Ginkgo biloba extract modulated the expression of ␣B-crystallin and heat-shock proteins 70 and 90␣ but not other stressrelated genes. Furthermore, changes associated with DEX were found less in GBE-treated or GBE-primed TM cells. Conclusion: We showed that GBE, a nontoxic, antiapoptotic, herbal compound significantly suppressed steroid-induced IOP elevation in rabbits and it seems to prevent the adverse effects of DEX on TM cells. Clinical Relevance: Ginkgo biloba extract could be a therapeutic agent or dietary supplement to prevent steroidinduced ocular hypertension.

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Distribution of Myocilin and Extracellular Matrix Components in the Corneoscleral Meshwork of Human Eyes

Cited by 125 publications

References 34 publications

Extracellular matrix in the trabecular meshwork

Extracellular matrix in the trabecular meshwork

Genetic Etiologies of Glaucoma

Effect of Topical Ginkgo biloba Extract on Steroid-Induced Changes in the Trabecular Meshwork and Intraocular Pressure

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