Distribution of IgM, IgD and IgZ in mandarin fish, Siniperca chuatsi lymphoid tissues and their transcriptional changes after Flavobacterium columnare stimulation

Tian, Jingyun; Sun, Bin; Luo, Yanping; Zhang, Yongan; Nie, Pin

doi:10.1016/j.aquaculture.2008.11.023

Cited by 101 publications

(74 citation statements)

References 35 publications

(41 reference statements)

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“…In this context, analysis of Ig isotype expression demonstrated differential expression patterns between the spleen and the head kidney. In particular the head kidney of Atlantic salmon (Tadiso et al 2011) showed the highest expression of IgM, IgT and IgD followed by the spleen, while in the thymus all Ig transcript levels were much lower in accordance with previous studies (Stenvik and Jorgensen 2000;Stenvik et al 2001;Hirono et al 2003;Hansen et al 2005;Saha et al 2005;Tian et al 2009). In particular, IgM transcripts were most abundant followed by IgT, especially in the head kidney and spleen, as compared to IgD, indicating that IgT is the dominant Ig next to IgM (Tadiso et al 2011).…”

Section: The Head Kidney and Spleensupporting

confidence: 77%

“…In a recent report, the gene sequences of three different Ig heavy chains, namely IgM, IgD and IgZ, were cloned from mandarin fish and the distributions of these three kinds of Ig-producing cells were investigated in the intestine by in situ hybridization. The data confirmed that IgM-producing cells could only be detected at the submucosa and lamina propria of the posterior intestine, while no IgZ or IgD positive cells were found in the gut (Tian et al 2009). IgT, an immunoglobulin isotype of unknown function, was described as acting like a mucosal antibody in rainbow trout.…”

Section: The Gut-associated Lymphoid Tissuesupporting

confidence: 74%

“…In fact, IgM + cells have been detected in the gill filament epithelium of halibut and in spotted wolffish (Grøntvedt and Espelid 2003), while in cod IgM + cells were detected in connective tissue and blood plasma, similar to the findings in Atlantic salmon (Schrøder et al 1998;Haugarvoll et al 2008). Moreover, both IgM + and IgZ + B cells were described along the gill filaments of mandarin fish Siniperca chuatsi (Tian et al 2009), while IgZ + cells were also detected in the gill of fugu (Savan et al 2005). Interestingly, in salmonid interbranchial lymphoid tissue (Haugarvoll et al 2008;Koppang et al 2010), very few IgM + cells were detected while abundant T cells were embedded in a meshwork of epithelial cells, showing an organization that has no resemblance to that previously described in lymphoid tissues .…”

Section: The Gill-associated Lymphoid Tissuesupporting

confidence: 70%

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Immune Defences of Teleost Fish

Foey

Picchietti

2014

Aquaculture Nutrition

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Teleost fish have a well-established innate immune response that is vital for anti-pathogen responses in their antigen-rich environment. With the increase in scientific investigations and the availability of specific reagents, it is clear that these fish also possess pathogen/antigen-specific adaptive responses. These responses are comparable to those of higher organisms, responding to intracellular and extracellular resident pathogens, initiating cell-mediated immunity (CMI) and humoral defences, respectively. This chapter reviews the current understanding of teleost immunity, with particular emphasis on the tissues involved in immune development (thymus, head kidney and spleen) and those under most antigenic stimulation -the skin and mucosal surfaces. In this context, an understanding of teleost immune defences is important for our comprehension of the health benefits conferred by prebiotics and probiotics introduced in feed formulations.

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Section: The Head Kidney and Spleensupporting

confidence: 77%

Section: The Gut-associated Lymphoid Tissuesupporting

confidence: 74%

Section: The Gill-associated Lymphoid Tissuesupporting

confidence: 70%

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Immune Defences of Teleost Fish

Foey

Picchietti

2014

Aquaculture Nutrition

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“…columnare , with a similar pattern of increase in the expression of IgM , IgD and IgZ genes as that observed in the present study (Tian et al, 2009). Recently, Makesh et al (2015) examined IgM antibody levels, and IgM , IgD and IgT gene expression in the systemic and mucosal immune systems of rainbow trout following immunization with attenuated F .…”

Section: Discussionmentioning

confidence: 52%

Immunogenicity and protective role of antigenic regions from five outer membrane proteins of Flavobacterium columnare in grass carp Ctenopharyngodon idella

Luo

Liu

et al. 2016

Chin. J. Ocean. Limnol.

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Flavobacterium columnare causes columnaris disease in freshwater fi sh. In the present study, the antigenic regions of fi ve outer membrane proteins (OMPs), including zinc metalloprotease, prolyl oligopeptidase, thermolysin, collagenase and chondroitin AC lyase, were bioinformatically analyzed, fused together, and then expressed as a recombinant fusion protein in Escherichia coli . The expressed protein of 95.6 kDa, as estimated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis, was consistent with the molecular weight deduced from the amino acid sequence. The purifi ed recombinant protein was used to vaccinate the grass carp, Ctenopharyngodon idella . Following vaccination of the fi sh their IgM antibody levels were examined, as was the expression of IgM , IgD and IgZ immunoglobulin genes and other genes such as MHC Iα and MHC I I β , which are also involved in adaptive immunity. Interleukin genes ( IL ), including IL -1β , IL -8 and IL -10 , and type I and type II interferon ( IFN ) genes were also examined. At 3 and 4 weeks post-vaccination (wpv), signifi cant increases in IgM antibody levels were observed in the fi sh vaccinated with the recombinant fusion protein, and an increase in the expression levels of IgM , IgD and IgZ genes was also detected following the vaccinations, thus indicating that an adaptive immune response was induced by the vaccinations. Early increases in the expression levels of IL and IFN genes were also observed in the vaccinated fi sh. At four wpv, the fi sh were challenged with F . column a re , and the vaccinated fi sh showed a good level of protection against this pathogen, with 39% relative percent survival (RPS) compared with the control group. It can be concluded, therefore, that the fi ve OMPs, in the form of a recombinant fusion protein vaccine, induced an immune response in fi sh and protection against F . columnare .

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“…Polyclonal and monoclonal antibodies to fish Ig have been crucial for developing immunological tools to evaluate total and specific concentrations of serum antibodies produced in response to inoculated antigen and to monitor the occurrence and prevalence of pathogenic microorganisms (Crosbie & Nowak 2002, Beelen et al 2004, Grove et al 2006b, Li et al 2007, Rathore et al 2008, Suresh Babu et al 2008, Lim et al 2009, Santos et al 2009, Sood et al 2011, Boardman et al 2012, Hedfors et al 2012, Purcell et al 2012. Anti-immunoglobulin antibodies have also been used to characterize and study the ontogeny and tissue distribution of cells subsets bearing membrane bound Ig (Beelen et al 2004, Grove et al 2006a, Tian et al 2009 and in cross reactivity studies to gain information on phylogenetic relationship amongst different species (Swennes et al 2007, Rathore et al 2008, Bag et al 2009, Santos et al 2009). Studies on silver catfish Ig and reacting anti-Ig antibodies have not been reported yet and the lack of relevant reagents for immunological studies hampers further studies on this fish species, mainly those concerning the evaluation of the humoral immune response following immunization, and the functional characterization of Ig-bearing subsets of leukocytes.…”

Section: Introductionmentioning

confidence: 99%

Characterization of an IgM-like immunoglobulin from silver catfish (Rhamdia quelen) serum and its use for the production of polyclonal antibodies and development of immunoassays

Kreutz

Canova²,

Nied³

et al. 2016

Pesq. Vet. Bras.

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Knowledge on fish immunoglobulin (Ig) characteristics and the availability of monoclonal or polyclonal antibodies to fish Igs are essential to evaluate the humoral immune response and the Ig distribution on leukocyte cells. We demonstrated that silver catfish serum Ig is composed of one immunodominant H chain with approximately 75k Da and one L chain with approximately 28 kDa, similar to human IgM. Rabbit polyclonal antibodies to the catfish IgM-like Ig recognized both the H and L chain and were useful in developing an indirect ELISA to measure the production of antibodies in fish immunized with bovine serum albumin. Dot blot and western blot cross-reactivity studies indicated a wide degree of epitope sharing amongst Ig from several Siluriformes and Characiformes fish indigenous to Brazilian rivers. In these fish species, polyclonal antibodies reacted mostly with the H chain. The results presented here are central to the development of tools and strategies to investigate the antibody production to inoculated antigens and tissue distribution of Ig molecules in native fish species. Furthermore, because of the wide range of cross-reactivity, polyclonal antibodies to silver catfish IgM-like Ig might be used to develop immunoassays to measure the humoral immune response in other fish species.

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Distribution of IgM, IgD and IgZ in mandarin fish, Siniperca chuatsi lymphoid tissues and their transcriptional changes after Flavobacterium columnare stimulation

Cited by 101 publications

References 35 publications

Immune Defences of Teleost Fish

Immune Defences of Teleost Fish

Immunogenicity and protective role of antigenic regions from five outer membrane proteins of Flavobacterium columnare in grass carp Ctenopharyngodon idella

Characterization of an IgM-like immunoglobulin from silver catfish (Rhamdia quelen) serum and its use for the production of polyclonal antibodies and development of immunoassays

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