Distribution of <i>Malassezia</i>
 species in pityriasis versicolor and seborrhoeic dermatitis in Greece. Typing of the major pityriasis versicolor isolate <i>M. globosa</i>

Gaitanis, Georgios; Velegraki, Aristea; Alexopoulos, Evangelos C.; Chasapi, Vasiliki; Tsigonia, A.; Katsambas, Andreas

doi:10.1111/j.1365-2133.2005.07114.x

Cited by 90 publications

(81 citation statements)

References 32 publications

(39 reference statements)

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“…62 Gupta and Gaitanis reported a greater amount of M. globosa. 63,64 Tajima was the only author to report M. restricta as being the most common. 65 Nakabayashi and Sei found that children with SD had a greater amount of Malassezia (M. furfur and M. globosa) compared to children without the disease.…”

Section: Malassezia Sppmentioning

confidence: 99%

Dermatite seborreica

Sampaio

Mameri²,

Vargas

et al. 2011

An. Bras. Dermatol.

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Seborrheic dermatitis is a chronic relapsing erythematous scaly skin disease, the prevalence of which is around 1 to 3% of the general population in the United States. It has two incidence peaks, the first in the first three months of life and the second beginning at puberty and reaching its apex at 40 to 60 years of age. The prevalence of seborrheic dermatitis is higher in HIV-positive individuals and the condition tends to be more intense and refractory to treatment in these patients. Neurological disorders and other chronic diseases are also associated with the onset of seborrheic dermatitis. The currently accepted theory on the pathogenesis of this disease advocates that yeast of Malassezia spp., present on the skin surface of susceptible individuals, leads to a non-immunogenic irritation due to the production of unsaturated fatty acids deposited on the skin surface. This article provides a review of the literature on seborrheic dermatitis, focusing on immunogenetics, the clinical forms of the disease and its treatment. . Keywords: Dermatitis; Dermatitis, seborrheic; Eczema Resumo: A dermatite seborreica é uma doença eritêmato-escamativa de caráter crônico-recidivante que acomete entre 1 e 3% da população geral dos Estados Unidos. Possui dois picos de incidência -o primeiro, durante os três primeiros meses de vida, e o segundo, a partir da puberdade, atingindo seu ápice entre os 40 e 60 anos de idade. Os indivíduos HIV positivos têm maior prevalência da doença, que apresenta maior intensidade e tendência à refratariedade ao tratamento. Doenças neurológicas e outras doenças crônicas também estão associadas ao desenvolvimento da dermatite seborreica. Como mecanismo fisiopatogênico, reconhece-se que o fungo Malassezia sp., presente na pele de indivíduos suscetíveis, leve a uma irritação não-imunogênica a partir da produção de metabólitos à base de áci-dos graxos insaturados deixados na superfície cutânea. Este artigo faz uma revisão da literatura sobre dermatite seborreica, com ênfase nos aspectos imunogenéticos, formas clínicas e tratamento.

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Section: Malassezia Sppmentioning

confidence: 99%

Dermatite seborreica

Sampaio

Mameri²,

Vargas

et al. 2011

An. Bras. Dermatol.

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“…To overcome the limits of morphological assessment, recent studies have used a variety of molecular methods such as the nested polymerase chain reaction (PCR) 12 , real-time PCR 13 , pulsed field gel electrophoresis (PFGE) 14 , amplified fragment length polymorphism (AFLP) 15,16 , denaturing gradient gel electrophoresis (DGGE) 16 , random amplification of polymorphic DNA (RAPD) 16,17 , single strand conformation polymorphism (SSCP) 18 , terminal fragment length polymorphism (tFLP) 19 , restriction fragment length polymorphism (RFLP) [20][21][22][23] , and sequencing analysis 24 .…”

Section: Introductionmentioning

confidence: 99%

Comparison of Nested PCR and RFLP for Identification and Classification of Malassezia Yeasts from Healthy Human Skin

Song

Lee

et al. 2009

Ann Dermatol

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Background: Malassezia yeasts are normal flora of the skin found in 75∼98% of healthy subjects. The accurate identification of the Malassezia species is important for determining the pathogenesis of the Malassezia yeasts with regard to various skin diseases such as Malassezia folliculitis, seborrheic dermatitis, and atopic dermatitis. Objective: This research was conducted to determine a more accurate and rapid molecular test for the identification and classification of Malassezia yeasts. Methods: We compared the accuracy and efficacy of restriction fragment length polymorphism (RFLP) and the nested polymerase chain reaction (PCR) for the identification of Malassezia yeasts. Results: Although both methods demonstrated rapid and reliable results with regard to identification, the nested PCR method was faster. However, 7 different Malassezia species (1.2%) were identified by the nested PCR compared to the RFLP method. Conclusion: Our results show that RFLP method was relatively more accurate and reliable for the detection of various Malassezia species compared to the nested PCR. But, in the aspect of simplicity and time saving, the latter method has its own advantages. In addition, the 26S rDNA, which was targeted in this study, contains highly conserved base sequences and enough sequence variation for inter-species identification of Malassezia yeasts. (Ann Dermatol 21(4) 352

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“…Variable M. furfur subtypes (Figure 1: strains GS4A/GS4B and GS9A/GS9B) from different anatomical regions showed different M13 fingerprints. As multiple Malassezia species are isolated from individuals (Gaitanis et al, 2006b;Tajima et al, 2008), an analogous intra-species polymorphism is expected.…”

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confidence: 99%

Malassezia furfur fingerprints as possible markers for human phylogeography

et al. 2008

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Malassezia furfur was the first species described within the cosmopolitan yeast genus Malassezia, which now comprises 13 species. Reported isolation rates of these species from healthy and diseased human skin show geographic variations. PCR-fingerprinting with the wild-type phage M13 primer (5 0 -GAGGGTGGCGGTTCT-3 0 ) was applied to investigate phylogeographic associations of M. furfur strains isolated from Scandinavians residing permanently in Greece, in comparison to clinical isolates from Greek, Bulgarian and Chinese native residents. Seven M. furfur strains from Scandinavians were compared with the Neotype strain (CBS1878), CBS global collection strains (n ¼ 10) and clinical isolates from Greece (n ¼ 4), Bulgaria (n ¼ 15) and China (n ¼ 6). Scandinavian, Greek and Bulgarian M. furfur strains mostly formed distinct group clusters, providing initial evidence for an association with the host's geographical origin and with the underlying skin condition. These initial data address the hypothesis that M. furfur could be a eukaryotic candidate eligible for phylogeographic studies.

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Distribution of Malassezia species in pityriasis versicolor and seborrhoeic dermatitis in Greece. Typing of the major pityriasis versicolor isolate M. globosa

Abstract: Malassezia species isolation rates were in agreement with those reported from South-west Europe. PCR-SSCP of the ITS 1 is useful for highlighting prospective clinical implications of M. globosa subtypes.

Cited by 90 publications

References 32 publications

Dermatite seborreica

Dermatite seborreica

Comparison of Nested PCR and RFLP for Identification and Classification of Malassezia Yeasts from Healthy Human Skin

Malassezia furfur fingerprints as possible markers for human phylogeography

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