Distribution of hepatitis B virus genotypes and viral load levels in Brazilian chronically infected patients in São Paulo city

Abstract

SUMMARYThe objective of the present study was to evaluate the serum viral load in chronically infected Hepatitis B virus (HBV) patients and to investigate the distribution of HBV genotypes in São Paulo city. Quantitative HBV-DNA assays and HBV genotyping have gained importance for predicting HBV disease progression, have been employed for assessing infectivity, for treatment monitoring and for detecting the emergence of drug resistance. Twenty-nine Brazilian patients with suspected chronic hepatitis B were studied, using real time PCR for viral load determination and direct DNA sequencing for the genotyping. The serology revealed chronic HBV infection in 22 samples. The HBV-DNA was positive in 68% samples (15/22). The phylogenetic analysis disclosed that eleven patients were infected with HBV genotype A, two with genotype F and two with genotype D. Thus, the genotype A was the most prevalent in our study.

Hepatitis B virus infection in children, adolescents, and their relatives: genotype distribution and precore and core gene mutations

“…Sitnik et al, studying the prevalence of HBV genotypes in chronic carriers from different regions of Brazil, also found genotype C in individuals of Asian origin 5,17 . Our results agree with other Brazilian studies [18][19][20][21][22] showing that genotypes A and D were the most frequent in the general population. It is interesting to observe that viral load was higher in genotype D carriers than in the other carriers.…”

Prevalence and clinical features of celiac disease in patients with hepatitis B virus infection in Southern Brazil

“…Palumbo et al 23 , found that 53.4% οf patients had HBV-DNA levels between 4,600 and 260,000IU/mL 23 . In São Paulo, 27% of patients had HBV-DNA levels higher than 2,000IU/mL, and 9% had HBV-DNA levels higher than 20,000IU/mL 25 . A study that included four of the fi ve Brazilian regions (the South, Southeast, Midwest and Northeast) found undetectable HBV-DNA levels in 21.6% of patients, HBV-DNA levels lower than 2,000IU/mL in 45.9% of patients, HBV-DNA levels between 2,000 and 20,000IU/mL in 12.6% of patients and HBV-DNA levels higher than 20,000IU/mL in 19.8% of patients 26 .…”

“…Following an evaluation of serum HBV-DNA, the prevalence of undetectable levels of HBV-DNA (< 20IU/mL) was 46.6% among immigrants in Italy 23 , 21.7% among pregnant women in Amazonas 24 and 32% in São Paulo 25 . In a study by Kiesslich et al 24 , quantitative analysis of HBV-DNA showed that 73.9% of pregnant women had levels lower than 200IU/mL, 14.4% had levels between 200 and 20,000IU/mL and 8.7% had levels higher than 20,000IU/mL 24 .…”

Avaliação de marcadores de lesão do túbulo proximal renal e incidência de redução da filtração glomerular em pacientes portadores de Hepatite B em uso de tenofovir

“…2 ) e o índice proteína urinária por creatinina urinária em amostra isolada de urina (Uprot/Ucreat Nas situações em que houver contraindicação ao uso de tenofovir ou quando se tenha desenvolvido alteração da função renal determinada por seu uso, deve ser indicado o tratamento com entecavir (Alcalde et al, 2009). …”