1993
DOI: 10.1111/j.1460-9568.1993.tb00965.x
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Abstract: The peripheral membrane protein gephyrin copurifies with the inhibitory glycine receptor of mammalian spinal cord. It binds with high affinity to polymerized tubulin and has been implicated in the anchoring of the glycine receptor to cytoskeletal elements. Recently, cDNA cloning has identified variants of the gephyrin mRNA, which originate from alternative splicing of four exonic regions (cassettes 1-4). In this study, the expression patterns of gephyrin splice variants were determined in the adult and develop… Show more

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Cited by 84 publications
(67 citation statements)
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“…That gephyrin exists in multiple isoforms has been established for some time (25,28,33); the most common variants have insertions in the "linker" region, where exon C3 inserts the peptide NHPFYTSPAVFMANHGQPIPGLISYSHHATGSA-DKR between amino acids 243 and 244, and the C4 exon inserts the sequence ARLPSCSSTYSVSE between amino acids 288 and 289. More recently, Ramming et al (25) described seven alternatively expressed murine exons including three consecutive C4 exons and two further 5Ј exons designated C6 and C7.…”
Section: Resultsmentioning
confidence: 99%
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“…That gephyrin exists in multiple isoforms has been established for some time (25,28,33); the most common variants have insertions in the "linker" region, where exon C3 inserts the peptide NHPFYTSPAVFMANHGQPIPGLISYSHHATGSA-DKR between amino acids 243 and 244, and the C4 exon inserts the sequence ARLPSCSSTYSVSE between amino acids 288 and 289. More recently, Ramming et al (25) described seven alternatively expressed murine exons including three consecutive C4 exons and two further 5Ј exons designated C6 and C7.…”
Section: Resultsmentioning
confidence: 99%
“…Gephyrin, therefore, has a synaptic function as a postsynaptic anchoring and clustering protein in neurons while facilitating a highly conserved metabolic purpose in nonneuronal tissues (32). The genetic and structural basis of the functional dichotomy is not established; however, in rats, a putative explanation may lie in the generation of distinct transcript isoforms of gephyrin constructed from alternative splicing of eight exonic "cassettes" within four regions of GPHN (25,28,33). Apart from conferring neuronal and nonneuronal characteristics to the mature polypeptide products, the purpose of the transcript heterogeneity pattern is unclear, and the number of isoform combinations in human tissue remains unestablished.…”
Section: Gephyrin (Gphn) Is An Organizational Protein That Clusters Amentioning
confidence: 99%
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“…Murine multiple tissue Northern blot membranes (CLONTECH) were probed with synthetic oligonucleotides corresponding to either the 3Ј end of the gephyrin coding region (T1) or cassettes C1-C4 (26). Gephyrin transcripts containing cassettes C5-C7 were detected with the following antisense probes: 5Ј-TGGACTGGACTCTTAAGTCGTAGC-CTCTATTCCTCCAGAGTTCAT-3Ј (C5), 5Ј-ATTCCTCT-GTGGTCTCTGCT TCAGT TCCTGCCT TGAGTGCCT-GCC-3Ј (C6), and 5Ј-ATCTGAGGCTACTTTCTTGTTGG-TATGTGTACCTCAGCCTGCTGC-3Ј (C7).…”
Section: Methodsmentioning
confidence: 99%