Distribution of Aminoglycoside Resistance Genes Among Acinetobacter Baumannii Strains Isolated From Burn Patients in Tehran, Iran

Khoshnood, Saeed; Eslami, Gita; Hashemi, Ali; Bahramian, Aghil; Heidary, Mohsen; Yousefi, Neda; Mohammdi, Fariba; Goli, Hamid Reza

doi:10.5812/pedinfect.57263

Cited by 13 publications

(10 citation statements)

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“…As expected, the most effective antibiotic with appreciable activity against the studied isolates was colistin. In agreement with our data, the results of previous studies in Iran ( 29 , 34 , 35 ) have shown that colistin-based therapy for treatment of diseases caused by MDR-AB had an appropriate clinical manifestation and decreased mortality.…”

Section: Discussionsupporting

confidence: 93%

Frequency of 16S rRNA Methylase and Aminoglycoside-Modifying Enzyme Genes among Clinical Isolates of Acinetobacter baumannii in Iran

Gholami¹,

Haghshenas²,

Moshiri³

et al. 2017

Iran J Pathol

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Background & objective: Multidrug-resistant Acinetobacter baumannii (MDR-AB) is an important nosocomial pathogen which is associated with significant morbidity and mortality, particularly in high-risk populations. Aminoglycoside-modifying enzymes (AMEs) and 16S ribosomal RNA (16S rRNA) methylation are two important mechanisms of resistance to aminoglycosides. The aim of this study was to determine the prevalence of 16S rRNA methylase ( armA , rmtA , rmtB , rmtC , and rmtD ), and the AME genes [ aac(6′)-Ib , aac(3)-I , ant(3′′)-I , aph(3′)-I and aac(6')-Id ], among clinical isolates of A. baumannii in Tehran, Iran. Methods: Between November 2015 to July 2016, a total of 110 clinical strains of A. baumannii were isolated from patients in two teaching hospitals in Tehran, Iran. Antimicrobial susceptibility testing was performed according to Clinical and Laboratory Standards Institute guidelines. The presence of genes encoding the AMEs and 16S rRNA methylases responsible for resistance was investigated by multiplex polymerase chain reaction. Results: The results showed that colistin was an effective antibiotic and could be used as a last-resort treatment of infections caused by MDR-AB . The resistance rate to aminoglycosides were 100%, 96.36% and 90.9% for tobramycin, gentamicin and amikacin, respectively. In this study, AME genes of aac(6′)-Ib , aac(3)-I and ant(3′′)-I were most prevalent among the isolated strains. Conclusion Markedly high resistance to tobramycin, gentamicin and amikacin was noted in current study. Our results suggested that modifying enzyme genes in conjunction with methylation of 16S rRNA might contribute to aminoglycoside resistance induced in vivo in A. baumannii . Further studies are required to determine the prevalence of the aminoglycoside resistance genes in other hospitals of Iran.

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Section: Discussionsupporting

confidence: 93%

Frequency of 16S rRNA Methylase and Aminoglycoside-Modifying Enzyme Genes among Clinical Isolates of Acinetobacter baumannii in Iran

Gholami¹,

Haghshenas²,

Moshiri³

et al. 2017

Iran J Pathol

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“…Then, DNA templates were prepared using the DNA extraction kit (Genet Bio Company, Korea, Cat. No, K-3000) as previously described 18…”

Section: Methodsmentioning

confidence: 99%

Molecular characterization of the <em>pilS2 </em>gene and its association with the frequency of <em>Pseudomonas aeruginosa </em>plasmid pKLC102 and PAPI-1 pathogenicity island

Bahramian

Khoshnood

Shariati

et al. 2019

IDR

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IntroductionPseudomonas aeruginosa is the most common opportunistic pathogen associated with a broad range of infections, including cystic fibrosis, ocular, otitis media, and burn infections. The aim of this study was to show the frequency of the pilS2 gene, and its association with P. aeruginosa plasmid pKLC102 and PAPI-1 pathogenicity island among P. aeruginosa strains.MethodsThe samples were collected from patients with cystic fibrosis, ocular, otitis media, and burn infections between January 2016 and November 2017. DNA was extracted using the DNA extraction kit and was used for PCR assay. PCR with 4 primer-pairs including 976 F/PAPI-1R, 4542 F/intF, SojR/4541 F, and intF/sojR was performed to identify PAPI-1. pKLC102 was detected using three other primer-pairs including cp10F/cp10R, cp44F/cp44R, and cp97F/cp97R.ResultsA total of 112 P. aeruginosa isolates were collected from patients with cystic fibrosis (36), burn (20), otitis media (26), and ocular (30) infections. The results of PCR showed that pilS2 gene was identified in 96 (85%) strains. PAPI-1–attB integration was detected among 38 (33.9%) isolates and the circular form of PAPI-1 detected among 17 (14%) isolates. In addition, 79 (70.5%) strains were found to be positive for pKLC102.ConclusionWe found that the majority of the isolates may be susceptible to transfer this significant island and the related element pKLC102 into recipient isolates lacking the island owing to high association of the PilS2 pilus with the islands in the studied strains. It is anticipated that strains isolated from burn and eye with the highest rate of PilS2, PAPI-1, and pKLC102 association have a high level of antibiotic resistance.

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“…Therefore, prolonged hospitalization in the intensive care unit, severe illnesses, immunodeficiency conditions, burn injuries, long-term use of antimicrobial agents, and catheter use are some of the most important risk factors for Acinetobacter infection [ 5 ]. A. baumannii is an important opportunistic pathogen responsible for a variety of nosocomial infections; including ventilator-associated pneumonia, bacteremia, surgical site infections, secondary infections of burn patients, secondary meningitis, and urinary tract infections in the Middle East region [ 6 , 7 ] and other parts of the world [ 8 , 9 ]. Hydrolysis of the β-lactam ring structure by ESBL causes an abnormal antibiotic function.…”

Section: Introductionmentioning

confidence: 99%

The Frequency of Antibiotic Resistance and ESBLs Among Clinically Acinetobacter baumannii Strains Isolated from Patients in a Major Hospital in Tehran, Iran

Ranjbar¹,

Tolon²,

Zayeri³

et al. 2018

TOMICROJ

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Background:Bacterial resistance to antibiotics limits treatment options, increases morbidity and mortality, and raises the risk of antibiotic-associated adverse events. Antibacterial resistance emerges rapidly following an increase in the consumption of antibiotics against infectious diseases. The spread of ESBL producing strains has a limiting factor based on antibiotic function for the treatment of infections particularly caused by Acinetobacter baumannii (A. baumannii).Objective:This study was conducted to evaluate the prevalence of antimicrobial resistance and distribution of blaTEM, blaCTX, and blaSHV genes among A. baumannii strains isolated from clinical samples at a major hospital in Teheran, Iran.Methods: A. baumannii strains were isolated and identified using standard microbiological methods. The disc diffusion and combined discs methods were used for testing antimicrobial susceptibility and to identify the strains producing Extended-Spectrum Beta-Lactamases (ESBL), respectively. DNA extraction was done by boiling method. Finally, the frequency of resistant genes including blaTEM, blaCTX, and blaSHV in ESBL producing isolates was studied by PCR.Results:Gender distribution in this study was 53 (53%) samples for men and 47 (47%) for women. Totally, one hundred A. baumannii strains were isolated. More than 93% of the isolates were multi drug resistant. The highest to lowest antibiotic resistance was observed against amoxicillin/clavulanic acid (98%), ceftriaxone (96%), cefotaxime (94%), and ceftazidime (93%), respectively. The frequency of positive phenotypic test of ESBL was 19% and 16% for CAZ-C and CTX-C, respectively. The frequency of blaTEM, blaCTX, and blaSHV genes was 52.1, 43.4, and 21.7, respectively.Conclusion: A. baumannii isolates exhibited an extremely worrying level of antibiotic resistance, and a high percentage of the isolates showed MDR in this study. This is a serious warning because ESBLs are a major threat to the effectiveness of antibiotics that are currently available for medical uses. The frequency of genes encoded ESBL isolates of A. baumannii may be due to overuse and misuse of antibiotics.

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Distribution of Aminoglycoside Resistance Genes Among Acinetobacter Baumannii Strains Isolated From Burn Patients in Tehran, Iran

Cited by 13 publications

References 20 publications

Frequency of 16S rRNA Methylase and Aminoglycoside-Modifying Enzyme Genes among Clinical Isolates of Acinetobacter baumannii in Iran

Frequency of 16S rRNA Methylase and Aminoglycoside-Modifying Enzyme Genes among Clinical Isolates of Acinetobacter baumannii in Iran

Molecular characterization of the <em>pilS2 </em>gene and its association with the frequency of <em>Pseudomonas aeruginosa </em>plasmid pKLC102 and PAPI-1 pathogenicity island

The Frequency of Antibiotic Resistance and ESBLs Among Clinically Acinetobacter baumannii Strains Isolated from Patients in a Major Hospital in Tehran, Iran

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