Distribution of 5-HT2A receptor immunoreactivity in the rat amygdaloid complex and colocalization with γ-aminobutyric acid

Functional neuroanatomy of monoaminergic systems in the basolateral nuclear complex of the amygdala: Neuronal targets, receptors, and circuits

“…IHC localization of the 5-HT2 A R has been complicated by staining differences produced by different antibodies et al, 2009). These results are also consistent with the findings of Bombardi (2011) in rats, who found that the great majority of BNC GABA+ INs were 5-HT2 A R+.…”

“…Using an antibody to 5‐HT2 A Rs different from the ones used by McDonald and Mascagni also demonstrated 5‐HT2 A R‐ir in PV neurons of the BNC (Jiang et al, 2009). These results are also consistent with the findings of Bombardi (2011) in rats, who found that the great majority of BNC GABA+ INs were 5‐HT2 A R+.…”

Functional neuroanatomy of monoaminergic systems in the basolateral nuclear complex of the amygdala: Neuronal targets, receptors, and circuits

“…IHC localization of the 5-HT2 A R has been complicated by staining differences produced by different antibodies et al, 2009). These results are also consistent with the findings of Bombardi (2011) in rats, who found that the great majority of BNC GABA+ INs were 5-HT2 A R+.…”

“…Using an antibody to 5‐HT2 A Rs different from the ones used by McDonald and Mascagni also demonstrated 5‐HT2 A R‐ir in PV neurons of the BNC (Jiang et al, 2009). These results are also consistent with the findings of Bombardi (2011) in rats, who found that the great majority of BNC GABA+ INs were 5‐HT2 A R+.…”

Functional anatomy of 5-HT2A receptors in the amygdala and hippocampal complex: relevance to memory functions

“…This result is in disagreement with in situ hybridization studies, showing that intercalated nuclei do not present 5-HT 2A R mRNA (Pompeiano et al 1994). Finally, pyramidal and nonpyramidal neurons of the rat amygdalohippocampal area contain the 5-HT 2A R (Bombardi 2011). …”

“…Different immunohistochemical studies have reported that the deep nuclei of the rat amygdaloid complex contain 5-HT 2A R-IR pyramidal and nonpyramidal neurons (Morilak et al 1993;Cornea-Hébert et al 1999;Xu and Pandey 2000;McDonald and Mascagni 2007;Jiang et al 2008;Bombardi 2011). These studies correlate with the evidence from an in situ hybridization study showing that 5-HT 2 R mRNA is located in the lateral, basal, and accessory basal nuclei (wright et al 1995).…”

Neuronal localization of 5-HT2A receptor immunoreactivity in the rat hippocampal region

“…(1) As observed in previous immunohistochemical studies [47,10,53], the 5-HT2Ar immunoreactivity obtained with the Pharmingen monoclonal antibody was colchicine-sensitive, thus comparing immunostaining of treated and untreated rats. (2) Colchicine impairs axonal and dendritic transport by disrupting microtubules and, consequently, causes 5-HT2Ar accumulation in the somata and proximal dendrites, enhancing the 5-HT2Ar immunoreactivity in these neuronal compartments [10,47,53]. Thus, colchicine treatment allows the identification of the morphology of the 5-HT2Arimmunoreactive (IR) neurons in Pharmingen antibody preparations.…”

Functional neuroanatomy of monoaminergic systems in the basolateral nuclear complex of the amygdala: Neuronal targets, receptors, and circuits

“…IHC localization of the 5-HT2 A R has been complicated by staining differences produced by different antibodies et al, 2009). These results are also consistent with the findings of Bombardi (2011) in rats, who found that the great majority of BNC GABA+ INs were 5-HT2 A R+.…”

“…Using an antibody to 5‐HT2 A Rs different from the ones used by McDonald and Mascagni also demonstrated 5‐HT2 A R‐ir in PV neurons of the BNC (Jiang et al, 2009). These results are also consistent with the findings of Bombardi (2011) in rats, who found that the great majority of BNC GABA+ INs were 5‐HT2 A R+.…”

Functional anatomy of 5-HT2A receptors in the amygdala and hippocampal complex: relevance to memory functions

“…This result is in disagreement with in situ hybridization studies, showing that intercalated nuclei do not present 5-HT 2A R mRNA (Pompeiano et al 1994). Finally, pyramidal and nonpyramidal neurons of the rat amygdalohippocampal area contain the 5-HT 2A R (Bombardi 2011). …”

“…Different immunohistochemical studies have reported that the deep nuclei of the rat amygdaloid complex contain 5-HT 2A R-IR pyramidal and nonpyramidal neurons (Morilak et al 1993;Cornea-Hébert et al 1999;Xu and Pandey 2000;McDonald and Mascagni 2007;Jiang et al 2008;Bombardi 2011). These studies correlate with the evidence from an in situ hybridization study showing that 5-HT 2 R mRNA is located in the lateral, basal, and accessory basal nuclei (wright et al 1995).…”

Neuronal localization of 5-HT2A receptor immunoreactivity in the rat hippocampal region

“…(1) As observed in previous immunohistochemical studies [47,10,53], the 5-HT2Ar immunoreactivity obtained with the Pharmingen monoclonal antibody was colchicine-sensitive, thus comparing immunostaining of treated and untreated rats. (2) Colchicine impairs axonal and dendritic transport by disrupting microtubules and, consequently, causes 5-HT2Ar accumulation in the somata and proximal dendrites, enhancing the 5-HT2Ar immunoreactivity in these neuronal compartments [10,47,53]. Thus, colchicine treatment allows the identification of the morphology of the 5-HT2Arimmunoreactive (IR) neurons in Pharmingen antibody preparations.…”

Functional neuroanatomy of monoaminergic systems in the basolateral nuclear complex of the amygdala: Neuronal targets, receptors, and circuits

“…IHC localization of the 5-HT2 A R has been complicated by staining differences produced by different antibodies et al, 2009). These results are also consistent with the findings of Bombardi (2011) in rats, who found that the great majority of BNC GABA+ INs were 5-HT2 A R+.…”

“…Using an antibody to 5‐HT2 A Rs different from the ones used by McDonald and Mascagni also demonstrated 5‐HT2 A R‐ir in PV neurons of the BNC (Jiang et al, 2009). These results are also consistent with the findings of Bombardi (2011) in rats, who found that the great majority of BNC GABA+ INs were 5‐HT2 A R+.…”

Functional anatomy of 5-HT2A receptors in the amygdala and hippocampal complex: relevance to memory functions

“…This result is in disagreement with in situ hybridization studies, showing that intercalated nuclei do not present 5-HT 2A R mRNA (Pompeiano et al 1994). Finally, pyramidal and nonpyramidal neurons of the rat amygdalohippocampal area contain the 5-HT 2A R (Bombardi 2011). …”

“…Different immunohistochemical studies have reported that the deep nuclei of the rat amygdaloid complex contain 5-HT 2A R-IR pyramidal and nonpyramidal neurons (Morilak et al 1993;Cornea-Hébert et al 1999;Xu and Pandey 2000;McDonald and Mascagni 2007;Jiang et al 2008;Bombardi 2011). These studies correlate with the evidence from an in situ hybridization study showing that 5-HT 2 R mRNA is located in the lateral, basal, and accessory basal nuclei (wright et al 1995).…”

Neuronal localization of 5-HT2A receptor immunoreactivity in the rat hippocampal region

“…(1) As observed in previous immunohistochemical studies [47,10,53], the 5-HT2Ar immunoreactivity obtained with the Pharmingen monoclonal antibody was colchicine-sensitive, thus comparing immunostaining of treated and untreated rats. (2) Colchicine impairs axonal and dendritic transport by disrupting microtubules and, consequently, causes 5-HT2Ar accumulation in the somata and proximal dendrites, enhancing the 5-HT2Ar immunoreactivity in these neuronal compartments [10,47,53]. Thus, colchicine treatment allows the identification of the morphology of the 5-HT2Arimmunoreactive (IR) neurons in Pharmingen antibody preparations.…”