Distribution of 35S-taurine in rat neonates and adults

Shimada, M.; Shimono, Ryuichi; Watanabe, Masaru; T, Imahayashi; Ozaki, Hiroki S.; Kihara, Takashi; Yamagata, Kenji; Niizeki, Shiro

doi:10.1007/bf00495770

Cited by 15 publications

(9 citation statements)

References 25 publications

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“…At high magnification, we found that the living cells have two kinds of verruciform protrusions on the cell surface. In the previous study, we reported the ultrastructural observation of Cbattone/& antiqua and observed some mucocysts protruding from the cell membrane (Shimada et al, 1986). Therefore, it is conceivable that the verruciform protrusions seen in the present work might be the heads of mucocysts located in the glycocalyx (Yokote and Honjo, 1985).…”

Section: Discussionsupporting

confidence: 53%

Localization of superoxide anion in the red tide alga Chattonella antiqua.

Shimada

Kawamoto²,

Nakatsuka³

et al. 1993

J Histochem Cytochem.

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To investigate the localization of superoxide anion generated in the red tide alga Chattonella antiqua, we added to the incubation medium 3,7-dihydro-6-[4-(2-(N'-(5-fluoresceinyl)thioureido) ethoxy)phenyl]-2-methylimidazo(1,2-a)pyrazin-3-one (FCLA), which is a Cypridina luciferin analogue and a specific fluorescent probe for detecting O2- and 1O2. The live phytoplankter was observed with the laser scanning microscope. At high magnification (x 6400) it was found that the plankter has many large and small verruciform protrusions on the cell surface. When the cell was irradiated with an argon laser (488 nm), a very intense fluorescence was noted at each protrusion. This fluorescence was completely inhibited by the addition of superoxide dismutase to the incubation medium, thus suggesting O2- generation at each verruciform protrusion on the cell surface.

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Section: Discussionsupporting

confidence: 53%

Localization of superoxide anion in the red tide alga Chattonella antiqua.

Shimada

Kawamoto²,

Nakatsuka³

et al. 1993

J Histochem Cytochem.

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“…In the hypophysis and pineal gland, the transporters involved are also unknown. The presence of very high radioactivity in the hypophysis and pineal gland after 14 C-GABA injection is of particular interest since taurine, which is structurally related to GABA, also strongly incorporates into these organs (Shimada et al, 1984). The functional relationship between taurine and GABA has been reviewed elsewhere (Huxtable, 1992).…”

Section: Discussionmentioning

confidence: 99%

Autoradiographic distribution of radioactivity from 14C-GABA in the mouse

Kuroda

Watanabe

Tamayama

et al. 2000

Microsc. Res. Tech.

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We investigated the distribution of radioactivity from (14)C-labeled gamma-aminobutyric acid (GABA) in the mouse by in vivo autoradiography to clarify the tissues that show GABA uptake and/or GABA binding. Male mice were injected intravenously with (14)C-GABA in both the absence and presence of an excess of unlabeled GABA, baclofen and isoguvacine. Whole-body autoradiography of (3)H-baclofen, a GABA(B) receptor agonist was also performed. At short intervals after (14)C-GABA injection ( 3 and 6 minutes), very high radioactivity was detected in the kidney cortex, liver, pineal gland, hypophysis, median eminence of the hypothalamus, and cervical ganglion. The hyaline cartilage and glandular part of the stomach showed moderate radioactivity. In the presence of an excess amount of unlabeled GABA, radioactivity in most of tissues decreased significantly, but no significant difference in radioactivity was observed in the presence of baclofen and isoguvacine, agonists of GABA(A) and GABA(B) receptors, respectively. Autoradiography of (3)H-baclofen showed that the kidney had high level of radioactivity, whereas the activity in other tissues and organs was similar or lower than in the blood except for the content of the urinary bladder and the pancreas at 15 minutes after injection. These data indicate that radioactivity from incorporated (14)C-GABA into a variety of cells is much higher than that from bound (14)C-GABA to the receptor sites. Our results suggest that GABA can be quickly localized in many organs of the mouse body after 3 minutes following injection, and GABA may serve multiple functions in those organs.

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“…Therefore, we investigated whether KCC2 may be inhibited by undefined embryonic factors. Taurine is a candidate due to enrichment in immature neurons during cerebral cortex development (30,31). In addition, taurine-deficient kittens show abnormal neuronal migration in the cerebral cortex and cerebellum (32,33).…”

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confidence: 99%