Distribution and morphological characteristics of the interstitial cells of Cajal in the ileocaecal junction of the guinea-pig

Miyamoto-Kikuta, Sachiko; Ezaki, Taichi; Komuro, Terumasa

doi:10.1007/s00441-009-0854-2

Cited by 13 publications

(9 citation statements)

References 22 publications

(32 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ileum protruded into the cecum, as reported [4] , who used human ICJ samples and reported that the muscle layers of the ileum and large intestine extended into the ileal papilla. In this study, we observed a thickening and narrowing of the smooth muscle layer in both groups, as reported [1][2][3][4]10] . Histologically, the elderly group exhibited a condensation of the connective tissue between smooth muscle cells in the transition; however, the young group displayed noticeable gaps between the smooth muscle fibers, which likely indicate limited development of collagen fibers.…”

Section: Discussionsupporting

confidence: 68%

“…There were foramens and laminar projections of collagen fibers in the ileum ( Figure 5C and D); however, the cecal The results for elaunin, which were obtained from samples stained with resorcin-fuchsin (Weigert), showed that the average linear density was 0.00692 ± 0.00015/ value of 20876.9 ± 60.4 pixels, a significant decrease of 3.8% (P < 0.001) compared to the young group ( Figure 6A). i.e., two circular muscle layers and a longitudinal layer, as also observed [10] . In the transition region, there was a thickening of the circular muscle layer, as previously observed [4] .…”

Section: Semsupporting

confidence: 61%

“…The ICJ includes the muscle bundles in the terminal ileum, the intrinsic nerve plexus, and the presence or absence of the interstitial cells of Cajal (ICC). Many ICC associated with the myenteric plexus are observed in both the ileal and cecal sides of the valve [10] . The neuronal density is lower in the cecum and ileal papilla compared to the terminal ileum.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of aging on the architecture of the ileocecal junction in rats

Brito

Chopard

Cury

et al. 2016

WJGPT

View full text Add to dashboard Cite

AIM:To evaluate the structural organization of the elastic and collagen fibers in the region of the ileocecal transition in 30 young and old male Wistar rats. METHODS:Histology, immunohistochemistry (IHC), transmission electron microscopy and scanning electron microscopy were employed in this study. The results demonstrated that there was a demarcation of the ileocecal region between the ileum and the cecum in both groups. RESULTS:The connective tissue fibers had different distribution patterns in the two groups. IHC revealed the presence of nitric oxide synthase, enteric neurons and smooth muscle fibers in the ileocecal junctions (ICJs) of both groups. Compared to the young group, the elderly group exhibited an increase in collagen type Ⅰ fibers, a decrease in collagen type Ⅲ fibers, a decreased linear density of oxytalan elastic fibers, and a greater linear density of elaunin and mature elastic fibers. CONCLUSION:The results revealed changes in the patterns of distribution of collagen and elastic fibers that may lead to a possible decrease in ICJ functionality. Core tip: The ileocolonic sphincter controls the forward and backward flow by integrating its motility with that of the distal ileum and proximal the ileocolonic sphincter controls the forward and backward flow by integrating its motility with that of the distal ileum and proxima. The ileocecal junction (ICJ) includes the muscle bundles in the terminal ileum, the intrinsic nerve plexus. The ICJ includes the muscle bundles in the terminal ileum, the intrinsic nerve plexus. Given the importance of knowing how the ICJ changes with age, the objective of this study was to characterize the morphological changes in the ICJ in rats aged 21 d and 2 years, using optical microscopy and electronic scanning and transmission methodologies. Additionally, the neurochemical characterization of the inhibitory neurons of the myenteric plexus, which are immunoreactive to the enzyme nitric oxide synthase, and the staining of the neuronal population were employed to identify immunoreactivity to HuC/D.de Brito MC, Chopard RP, Cury DP, Watanabe IS, Mendes CE, Castelucci P. Effects of aging on the architecture of the ileocecal junction in rats.

show abstract

Section: Discussionsupporting

confidence: 68%

Section: Semsupporting

confidence: 61%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of aging on the architecture of the ileocecal junction in rats

Brito

Chopard

Cury

et al. 2016

WJGPT

View full text Add to dashboard Cite

show abstract

“…In contrast to the ICC-SP of the stomach (Kunisawa and Komuro 2008), ileocaecal junction (Miyamoto-Kikuta et al 2009) and caecum (Tamada and Komuro 2011), ICC-SP and their processes in the proximal colon have been frequently observed in wide connective tissue spaces away from the submucosal ganglia. Basket-like structures composed of multipolar ICC-SP have been observed around the submucosal ganglia in the caecum of the guinea pig but these structures have not been observed in the proximal colon.…”

Section: Discussionmentioning

confidence: 97%

Ultrastructural characterization of interstitial cells of Cajal associated with the submucosal plexus in the proximal colon of the guinea pig

Tamada

Komuro

2012

Cell Tissue Res

Self Cite

View full text Add to dashboard Cite

Interstitial cells of Cajal (ICC) associated with the submucosal (submucous) plexus (ICC-SP) in the proximal colon of the guinea pig were studied by immunohistochemistry and electron microscopy. Whole-mount stretch preparations with c-Kit immunohistochemistry revealed that a number of ICC-SP constituted a dense cellular network around the submucosal plexus. Some of these ICC-SP were observed in the vicinity of the muscularis mucosae in sections immunostained for c-Kit and α-smooth muscle actin. Ultrastructural observation demonstrated, for the first time, that ICC-SP of the proximal colon of the guinea pig retained typical ultrastructural characteristics of ICC repeatedly reported in association with the tunica muscularis of the gastrointestinal tract: a basal lamina, caveolae, many mitochondria, abundant intermediate filaments and the formation of gap junctions with the same type of cells. The most remarkable ultrastructural finding was the presence of thick bundles composed of the processes of ICC-SP connected to each other via large gap junctions. These ICC-SP might be involved in the main mucosal functions of the proximal colon of the guinea pig, namely the transportation of water and electrolytes, possibly via their involvement in the spontaneous contractions of the muscularis mucosae.

show abstract

“…Recent studies have identified another ICC subtype associated with the submucosal plexus (ICC-SP) in the stomach (Kunisawa and Komuro 2008) and in the ileocaecal junction (Miyamoto-Kikuta et al 2009) of the guinea-pig. This unique ICC subtype is suggested to be involved in mucosal activity such as the secretion, absorption and transportation of fluids.…”

Section: Introductionmentioning

confidence: 98%

Three-dimensional demonstration of the interstitial cells of Cajal associated with the submucosal plexus in guinea-pig caecum

Tamada

Komuro

2011

Cell Tissue Res

Self Cite

View full text Add to dashboard Cite

The guinea-pig caecum was studied by using immunohistochemistry for Kit receptors and nerves to clarify whether interstitial cells of Cajal (ICC) were localized in association with the submucosal plexus (ICC-SP). A large area of the guinea-pig caecum was nearly devoid of longitudinal muscles, because they were concentrated into three bundles of the taenia caeci (coli) and this allowed clear observation of the myenteric and submucosal plexus as separate networks in whole-mount stretch preparations. The myenteric plexus was observed as a loose polygonal network consisting in elongated ganglia and long connecting nerve strands, whereas the submucosal plexus was identified as smaller ovoid ganglia connected to much thinner nerve strands in different tissue layers. Three-dimensional reconstruction of confocal images revealed multipolar-shaped ICC-SP located around the submucosal ganglion in a basket formation. Bipolar ICC-SP were also observed along the connecting nerve strands of the submucosal plexus. The functional involvement of ICC-SP in mucosal activity is discussed in relation to fluid transportation. This three-dimensional study of ICC-SP thus provides a candidate for the most suitable material available for functional experiments examining the physiological significance of ICC-SP.

show abstract

Distribution and morphological characteristics of the interstitial cells of Cajal in the ileocaecal junction of the guinea-pig

Cited by 13 publications

References 22 publications

Effects of aging on the architecture of the ileocecal junction in rats

Effects of aging on the architecture of the ileocecal junction in rats

Ultrastructural characterization of interstitial cells of Cajal associated with the submucosal plexus in the proximal colon of the guinea pig

Three-dimensional demonstration of the interstitial cells of Cajal associated with the submucosal plexus in guinea-pig caecum

Contact Info

Product

Resources

About