Distribution and Excretion of Bilberry Anthocyanins in Mice

Sakakibara, Hiroyuki; Ogawa, Takeshi; Koyanagi, Akiharu; Kobayashi, Saori; Goda, Toshinao; Kumazawa, Shigenori; Kobayashi, Hirokazu; Shimoi, Kayoko

doi:10.1021/jf901341b

Cited by 73 publications

(85 citation statements)

References 36 publications

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“…In mice, anthocyanins were detected in the liver but not in muscle or white fat tissue after the consumption of bilberry extract [40]. It has previously been shown that increased GFAT activity is associated with insulin resistance and postprandial hyperglycemia in T2D [41].…”

Section: Discussionmentioning

confidence: 89%

Polyphenol-Rich Bilberry Ameliorates Total Cholesterol and LDL-Cholesterol when Implemented in the Diet of Zucker Diabetic Fatty Rats

Brader¹,

Overgaard²,

Christensen³

et al. 2013

Rev Diabet Stud

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■ AbstractBACKGROUND: Bilberries and blackcurrants are nutrient sources rich in bioactive components, including dietary fibers, polyphenols, and anthocyanins, which possess potent cardiovascular protective properties. Few studies investigating the cardio-protective effects of natural components have focused on whole bilberries or blackcurrants. OBJECTIVE: The aim of this trial was to investigate whether a diet enriched with bilberries or blackcurrants has beneficial effects on glucose metabolism, lipid profile, blood pressure, and expression of genes related to glucose and lipid metabolism. METHODS: Male Zucker Diabetic Fatty (ZDF) rats (n = 48) were randomly assigned to either a control, bilberryenriched, blackcurrant-enriched, or fiber-enriched diet for 8 weeks ad libitum. Real-time quantitative PCR analysis was performed on liver, adipose, and muscle tissue. Berry polyphenol content was determined by HPLC and LC-MS analysis. RESULTS: Bilberry enrichment reduced total (-21%, p = 0.0132) and LDL-cholesterol (-60%, p = 0.0229) levels, but increased HDL-cholesterol to a lesser extent than in controls. This may partly be due to the altered hepatic liver X receptor-α expression (-24%, p < 0.001). Neither bilberries nor blackcurrants influenced glucose metabolism or blood pressure. Nevertheless, transcriptional analysis implied a better conservation of hepatic and adipocyte insulin sensitivity by bilberry enrichment. Anthocyanins constituted 91% and 87% of total polyphenol content in bilberries and blackcurrants, respectively. However, total anthocyanin content (3441 mg/100 g) was 4-fold higher in bilberries than in blackcurrants (871 mg/100 g). CONCLUSIONS: Bilberry consumption ameliorated total and LDL-cholesterol levels, but not HDL-cholesterol levels in ZDF rats. Neither bilberry nor blackcurrant enrichment delayed the development of diabetes or hypertension. Thus, in rats, bilberries may be valuable as a dietary preventive agent against hypercholesterolemia, probably by virtue of their high anthocyanin content.

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Section: Discussionmentioning

confidence: 89%

Polyphenol-Rich Bilberry Ameliorates Total Cholesterol and LDL-Cholesterol when Implemented in the Diet of Zucker Diabetic Fatty Rats

Brader¹,

Overgaard²,

Christensen³

et al. 2013

Rev Diabet Stud

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show abstract

“…The anthocyanin analysis was performed according to our previous method 18) with some modifications. Each plasma (1 mL) and urine (3 mL) sample was loaded onto an OASIS HLB (200 mg) extraction cartridge (Waters Co., Milford, MA, USA), which had been equilibrated with 10 mM oxalic acid.…”

mentioning

confidence: 99%

Practical application of flavonoid-poor menu meals to the study of the bioavailability of bilberry anthocyanins in human subjects

Sakakibara

Ichikawa

Tajima

et al. 2014

Bioscience, Biotechnology, and Biochemistry

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Practical application of flavonoid-poor menus was evaluated on the bioavailability of anthocyanins as model flavonoids. Detectable amounts of flavonoids were not found in plasma and urine collected from 13 participants, who took the menus. After ingesting bilberry anthocyanins (919 μmol), average plasma AUC0-6h, Cmax, Tmax values and urinary recovery were 386.0 nmol h/mL, 139.1 nM, 1.31 h and 0.21%, respectively.

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“…At 2 days after surgery, rats in the Ovx+VME group were administered VME by gavage at a dose of 500 mg/kg body weight (equivalent to 10 ml/kg body weight of 5% VME) daily for 8 weeks. The dose of VME was determined based on previous studies in rodents (40,41). Rats in the other two groups were given DW by gavage at a dose of 10 ml/kg body weight daily for 8 weeks.…”

Section: Animals and Dietsmentioning

confidence: 99%

Effect of anthocyanin-rich bilberry extract on bone metabolism in ovariectomized rats

et al. 2017

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Abstract. Menopause is associated with increased oxidative stress, which serves a role, in part, in the pathogenesis of postmenopausal bone loss. Fruits and vegetables are rich in antioxidative nutrients and phytochemicals. Berries are a natural source of anthocyanins, and their intake may improve bone health. The aim of the present study was to determine the effect of an anthocyanin-rich bilberry extract (VME) on bone metabolism in an ovariectomized (Ovx) rat. Female Sprague-Dawley rats (12 weeks old) were randomly divided into the following four groups: Baseline, Sham, Ovx and Ovx+VME (n=8-12 rats per group). Rats in the Baseline group were sacrificed immediately, while those in the other groups were subjected to either sham operation (Sham) or bilateral Ovx (Ovx and Ovx+VME). Rats in the Ovx+VME group were administered VME daily at a dose of 500 mg/kg body weight. At 8 weeks after surgery, bone mass and bone histomorphometry were evaluated. The femur bone mineral density (BMD) in the Ovx group was significantly lower than that in the Sham group (P<0.01). Supplementation of VME in the Ovx rats did not result in an increase in BMD. Histomorphometric analyses revealed that Ovx resulted in decreased measures of bone volume and trabecular number and increased measures of osteoid volume, mineralizing surface and bone formation rates (all P<0.01), whereas VME had no significant effects on these parameters. The present findings indicate that VME did not alter bone metabolism in Ovx rats, suggesting that consumption of VME may not be helpful in preventing postmenopausal bone loss.

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Distribution and Excretion of Bilberry Anthocyanins in Mice

Cited by 73 publications

References 36 publications

Polyphenol-Rich Bilberry Ameliorates Total Cholesterol and LDL-Cholesterol when Implemented in the Diet of Zucker Diabetic Fatty Rats

Polyphenol-Rich Bilberry Ameliorates Total Cholesterol and LDL-Cholesterol when Implemented in the Diet of Zucker Diabetic Fatty Rats

Practical application of flavonoid-poor menu meals to the study of the bioavailability of bilberry anthocyanins in human subjects

Effect of anthocyanin-rich bilberry extract on bone metabolism in ovariectomized rats

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