Distributed Features of Vimentin-Containing Neural Precursor Cells in Olfactory Bulb of SOD1G93A Transgenic Mice: a Study about Resource of Endogenous Neural Stem Cells

Tang, Chunyan; Zhu, Lei; Gan, Weiming; Liang, Huiting; Li, Jiao; Zhang, Jie; Zhang, Xiong; Lü, Yi; Xu, Renshi

doi:10.7150/ijbs.16696

Cited by 3 publications

(8 citation statements)

References 49 publications

(40 reference statements)

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“…9,[16][17][18][19][20][21][22] Although the SVZ is the main source of OB interneurons, a number of studies claimed local NSCs exist in the adult rodent and human OB. [23][24][25][26][27][28][29][30][31] Furthermore, it has been reported that cells derived from the human OB give rise to neurons and glia in cell culture. [32][33][34][35][36][37] However, the precise location, features, and most importantly, the full neurogenic potential of these NSCs in vivo has yet to be defined.…”

Section: Introductionmentioning

confidence: 99%

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Neural Stem Cells in the Adult Olfactory Bulb Core Generate Mature Neurons in Vivo

et al. 2021

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Although previous studies suggest that neural stem cells (NSCs) exist in the adult olfactory bulb (OB), their location, identity, and capacity to generate mature neurons in vivo has been little explored. Here, we injected enhanced green fluorescent protein (EGFP)-expressing retroviral particles into the OB core of adult mice to label dividing cells and to track the differentiation/maturation of any neurons they might generate. EGFP-labeled cells initially expressed adult NSC markers on days 1 to 3 postinjection (dpi), including Nestin, GLAST, Sox2, Prominin-1, and GFAP. EGFP +doublecortin (DCX) cells with a migratory morphology were also detected and their abundance increased over a 7-day period. Furthermore, EGFP-labeled cells progressively became NeuN + neurons, they acquired neuronal morphologies, and they became immunoreactive for OB neuron subtype markers, the most abundant representing calretinin expressing interneurons. OB-NSCs also generated glial cells, suggesting they could be multipotent in vivo. Significantly, the newly generated neurons established and received synaptic contacts, and they expressed presynaptic proteins and the transcription factor pCREB. By contrast, when the retroviral particles were injected into the subventricular zone (SVZ), nearly all (98%) EGFP + -cells were postmitotic when they reached the OB core, implying that the vast majority of proliferating cells present in the OB are not derived from the SVZ. Furthermore, we detected slowly dividing label-retaining cells in this region that could correspond to the population of resident NSCs. This is the first time NSCs located in the adult OB core have been shown to generate neurons that incorporate into OB circuits in vivo.

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Section: Introductionmentioning

confidence: 99%

“…Although the SVZ is the main source of OB interneurons, a number of studies claimed local NSCs exist in the adult rodent and human OB 23‐31 . Furthermore, it has been reported that cells derived from the human OB give rise to neurons and glia in cell culture 32‐37 .…”

Section: Introductionmentioning

confidence: 99%

Neural Stem Cells in the Adult Olfactory Bulb Core Generate Mature Neurons in Vivo

et al. 2021

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“…Our results showed that VCCs in the motor cortex, the olfactory cortex and the cingulate cortex significantly reduced and the number and density in the entire cerebrum didn't significantly increase. Previous studies reported that NPCs or NSCs in reduced regions of CNS migrated to damaged regions to restore neural cells at some pathological conditions 39, 40, 42, 52, 57. Therefore, we hypothesized that reduced VCCs in the motor cortex, the olfactory cortex and the cingulate cortex might migrate to other brain regions, and play a self-restoring ability to repair damaged neural cells in ALS-like Tg(SOD1*G93A)1Gur mice.…”

Section: Discussionmentioning

confidence: 91%

“…Current studies found that NPCs or NSCs in the brain and the spinal cord extensively proliferated, differentiated and migrated damaged regions to restore damaged neural cells at some pathological conditions such Parkinson's disease, Alzheimer's disease, spinal cord injury and ALS, partial NPCs or NSCs differentiated into astrocytes, oligodendrocytes and neuron cells, but the self-repair ability was very limited 39, 40, 42, 52, 57. However, in our study, the number and density of VCCs in the cerebrum of Tg(SOD1*G93A)1Gur mice weren't found to significantly increase at all stages of ALS-like Tg(SOD1*G93A)1Gur mice compared with WT mice.…”

Section: Discussionmentioning

confidence: 99%

Altered Features of Vimentin-containing Cells in Cerebrum of Tg(SOD1*G93A)1Gur Mice: A Preliminary Study on Cerebrum Endogenous Neural Precursor Cells in Amyotrophic Lateral Sclerosis

et al. 2019

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Vimentin-containing cells (VCCs) are potential neural precursor cells in central nervous systems, Thus, we studied the alteration of VCCs proliferation, differentiation and migration in the cerebrum during different stages of Tg(SOD1*G93A)1Gur mice. It aims to search potential ways regulating the proliferation, differentiation and migration of endogenous VCCs, to enhance their neural repair function and to cure or prevent from the development of ALS. We observed and analyzed the proliferation, differentiation and migration of VCCs in different anatomic regions and cell types of cerebrum at different stages including the pre-onset (60-70 days), onset (90-100 days) and progression (120-130 days) of wild-type (WT) and Tg(SOD1*G93A)1Gur mice using the fluorescent immunohistochemical technology. Results showed that VCCs in the cerebrum were mostly distributed in the ventricular system, periventricular structures, the hippocampus and the cerebral cortex in WT mice. VCCs significantly reduced in the motor cortex and the cingulate cortex in Tg(SOD1*G93A)1Gur mice. All vimentin expressed in the extranuclear and almost all VCCs were astrocytes in WT mice and Tg(SOD1*G93A)1Gur mice. There were no significant difference in the number of Brdu and nestin positive cells in left and right brains of WT mice and Tg(SOD1*G93A)1Gur mice in the period of 60-130 days. Our data suggested that there existed extensively NPCs in the cerebrum of adult mice. In ALS-like Tg(SOD1*G93A)1Gur mice, VCCs in the motor cortex, the olfactory cortex and the cingulate cortex showed that no any proliferation and redistribution in neural cells of VCCs in the cerebrum occurred in all stages of ALS, might migrate to damaged regions.

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“…The vimentin-containing cells (VCCs) in CNS are potential resources of NPCs or NSCs [20][21][22][23][24][25]. The aim of this study was to investigate the potential modulation and mechanism of proliferation, differentiation, and migration of endogenous NPCs or NSCs in CNS at the ALS pathological condition by observing and analyzing the altered features of VCCs in the ALS-like Tg(SOD1*G93A)1Gur mice.…”

Section: Introductionmentioning

confidence: 99%

Distribution Changes of Neural Precursor Cells in the Brain Stem of Tg(SOD1*G93A)1Gur Mice

et al. 2021

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Objectives: The alteration of vimentin-containing cells (VCCs) proliferation, differentiation and migration in the brain stem of amyotrophic lateral sclerosis (ALS)-like transgenic mice (Tg(SOD1*G93A)1Gur mice) (TG mice) and wild-type mice (WT mice) at the different disease stages of TG mice were studied in this study. The aim of this study was to investigate the change features of proliferation, differentiation and migration of endogenous neural precursor cells (NPCs) and to explore the potential effects of NPCs on restoring degenerated neurons in ALS. Methods: The proliferation, differentiation and migration of VCCs in both different anatomic regions and neural cells of brain stem at the different stages including pre-onset (60-70 days), onset (90-100 days) and progression (120-130 days) stages of TG mice and in WT mice (control) were examined using the immunofluorescence technology. Results: VCCs mainly distributed in the around (peripheral) central canal (CC) and the nuclei of brain stem in adult WT mice. VCCs proliferated and differentiated into astrocytes and directionally migrated from the around CC to the nuclei of brain stem, then to the ventral part of damaged regions in brain stem at the pre-onset, onset and progression stages of TG mice. Conclusions: The data suggest that NPCs widely distribute in the brain stem of adult TG mice can differentiate into astrocytes and migrate into damaged brain regions. This response might be a potential mechanism to repair degenerated motor neurons and restore dysfunctional neural circuitry in ALS.

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Distributed Features of Vimentin-Containing Neural Precursor Cells in Olfactory Bulb of SOD1G93A Transgenic Mice: a Study about Resource of Endogenous Neural Stem Cells

Cited by 3 publications

References 49 publications

Neural Stem Cells in the Adult Olfactory Bulb Core Generate Mature Neurons in Vivo

Neural Stem Cells in the Adult Olfactory Bulb Core Generate Mature Neurons in Vivo

Altered Features of Vimentin-containing Cells in Cerebrum of Tg(SOD1*G93A)1Gur Mice: A Preliminary Study on Cerebrum Endogenous Neural Precursor Cells in Amyotrophic Lateral Sclerosis

Distribution Changes of Neural Precursor Cells in the Brain Stem of Tg(SOD1*G93A)1Gur Mice

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