1984
DOI: 10.1016/s0006-3495(84)84169-7
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Abstract: Galactose and other chemotactic attractants have been shown to trigger an apparent hyperpolarization in Escherichia coli (Eisenbach, M., 1982, Biochemistry, 21:6818-6825). The probe used to measure membrane potential in that study, tetraphenylphosphonium (TPP+), may respond also to surface-charge changes in the membrane. The distinction between true changes in membrane potential and changes in the surface charge of the membrane is crucial for the study of this phenomenon in bacterial chemotaxis. To distinguish… Show more

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Cited by 8 publications
(4 citation statements)
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References 32 publications
(55 reference statements)
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“…Since in the absence of K+ valinomycin does not affect the PMF (3,17,47) or the swimming behavior of the bacteria (Table 4), it was hoped that in the presence of only 2 mM K+ (lower than the presumed internal concentration [6]) valinomycin would clamp Atwithout dissipating it. This was the observed result (Table 4).…”
Section: Chemicals [14c]tetraphenylphosphonium Bromide Wasmentioning
confidence: 99%
See 1 more Smart Citation
“…Since in the absence of K+ valinomycin does not affect the PMF (3,17,47) or the swimming behavior of the bacteria (Table 4), it was hoped that in the presence of only 2 mM K+ (lower than the presumed internal concentration [6]) valinomycin would clamp Atwithout dissipating it. This was the observed result (Table 4).…”
Section: Chemicals [14c]tetraphenylphosphonium Bromide Wasmentioning
confidence: 99%
“…The statistical significance of the change in adaptation time in high-K+ buffer (Table 3) or with B. subtilis was, however, doubtful (P s 90%). Since the average change in adaptation time was only 24%, it seems to us that this change may be the consequence of a secondary or an indirect effect of A%s on one of the processes involved in adaptation, e.g., the chemotaxis-related hyperpolarization (12,13,15,17) (cf. Fig.…”
Section: Chemicals [14c]tetraphenylphosphonium Bromide Wasmentioning
confidence: 99%
“…itself, however, that causes the tactic signal but instead it is the consequent increase in pmf. The primary signal involved in the chemotactic response is still unknown, but there is some evidence that there is a change in pmf on binding of the chemoeffector to the receptor in some bacterial species (2,8,9), although the evidence is conflicting and the time course of the chemotactic response suggests an additional requirement (16,17). We currently are investigating the interaction of chemotaxis and the electron transport taxes to identify the mechanism of interaction.…”
Section: Discussionmentioning
confidence: 99%
“…To analyze changes in the transmembrane potential, we used tetraphenylphosphonium (TPP), as described by Eisenbach et al [15].…”
Section: Methodsmentioning
confidence: 99%