Distinct cytokine use and production by T cells after activation by langerhans cells versus macrophages infiltrating UV-irradiated epidermis

Stevens, SR; Tarry-Lehmann, M; Cooper, K. D.

doi:10.1016/s0923-1811(98)83108-3

Cited by 3 publications

(3 citation statements)

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“…The T cells responding to these macrophages have a typical IL-2R␣ negative phenotype and the proliferation of these T cells appears to be dependent on IL-4 but not on IL-2, as measured by in vitro assays (48 -50). Furthermore, in comparison to control epidermal cell suspensions (containing LC), epidermal cells from UVB-irradiated skin (containing macrophages) stimulate higher numbers of allogeneic peripheral blood CD4 ϩ T cells to produce IL-4, as measured in primary and secondary cultures by an ELISA spot assay (51). In line with this reported Th2 shift, we demonstrated by means of intracellular FACS analysis a much higher IL-4 production in responding autologous CD4 ϩ T cells in primary dermal cell cultures derived from UVB-irradiated skin than in the T cell population from control skin.…”

Section: Figure 6 Depletion Of Cd15mentioning

confidence: 99%

Ultraviolet B Radiation Induces a Transient Appearance of IL-4+ Neutrophils, Which Support the Development of Th2 Responses

Teunissen

Pişkin

Nuzzo

et al. 2002

The Journal of Immunology

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UVB irradiation can cause considerable changes in the composition of cells in the skin and in cutaneous cytokine levels. We found that a single exposure of normal human skin to UVB induced an infiltration of numerous IL-4+ cells. This recruitment was detectable in the papillary dermis already 5 h after irradiation, reaching a peak at 24 h and declining gradually thereafter. The IL-4+ cells appeared in the epidermis at 24 h postradiation and reached a plateau at days 2 and 3. The number of IL-4+ cells was markedly decreased in both dermis and epidermis at day 4, and at later time points, the IL-4 expression was absent. The IL-4+ cells did not coexpress CD3 (T cells), tryptase (mast cells), CD56 (NK cells), and CD36 (macrophages). They did coexpress CD15 and CD11b, showed a clear association with elastase, and had a multilobed nucleus, indicating that UVB-induced infiltrating IL-4+ cells are neutrophils. Blister fluid from irradiated skin, but not from control skin, contained IL-4 protein as well as increased levels of IL-6, IL-8, and TNF-α. In contrast to control cultures derived from nonirradiated skin, a predominant type 2 T cell response was detected in T cells present in primary dermal cell cultures derived from UVB-exposed skin. This type 2 shift was abolished when CD15+ cells (i.e., neutrophils) were depleted from the dermal cell suspension before culturing, suggesting that neutrophils favor type 2 T cell responses in UVB-exposed skin.

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Section: Figure 6 Depletion Of Cd15mentioning

confidence: 99%

Ultraviolet B Radiation Induces a Transient Appearance of IL-4+ Neutrophils, Which Support the Development of Th2 Responses

Teunissen

Pişkin

Nuzzo

et al. 2002

The Journal of Immunology

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“…27 Furthermore, T cells stimulated by UV-exposed epidermal cells had altered growth factor utilization and cytokine production patterns. 28 T cells cultured with control epidermal cells utilized IL-2, IL-7 and IL-15 for growth, and produced predominantly interferon (IFN)-␥. Those stimulated with UV-exposed epidermal cells used IL-4 for growth, and more cells produced IL-4 and/or IL-5 compared with controls.…”

Section: Antigen-presenting Functionmentioning

confidence: 99%

The effects of ultraviolet radiation on the human immune system

Duthie

Kimber

Norval

1999

British Journal of Dermatology

209

162

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The adverse outcome of increased ultraviolet (UV) irradiation on human health is currently of concern. While many experiments have been carried out in rodent models, fewer have been designed to test the effects of UV exposure in human subjects. This review concentrates on the modulations induced in the human immune system by UV, and outlines changes in antigen presentation by Langerhans cells and macrophages, in the activities of natural killer cells and T cells, and in cytokine regulation. Precautionary measures which might be taken to help protect people against the immunosuppressive action of UV irradiation are considered.

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“…Proliferation of T cells activated by UV-M ¤ was dependent on IL-4 but not on IL-2 [9]. The IL-2 + IL-2R § − phenotype has been associated with immunological tolerance in several transplantation and cancer animal models.…”

Section: Introductionmentioning

confidence: 99%

Low expression of CD40 and B7 on macrophages infiltrating UV-exposed human skin; role in IL-2Rα− T cell activation

et al. 1998

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After UV exposure of skin, epidermal Langerhans cells (LC) are depleted, whereas CD11b + CD36 + CD1a − monocytes/macrophages (UV-M ¤) infiltrate. Different immunological outcomes in vivo are mediated by LC (sensitization) and UV-M ¤ (tolerance) which may be related to the distinct T cell activation states that these antigen-presenting cells (APC) induce. We previously demonstrated that CD4 + T lymphocytes activated by UV-M ¤ are, in contrast to LC-activated T cells, IL-2R § deficient, and we hypothesize that this differential T cell activation is related to differences in co-stimulatory molecules between UV-M ¤ and LC. Using four-color flow cytometry, we found a reduced capacity to up-regulate expression of the important co-stimulatory molecules CD40, B7-1 and B7-2 by UV-M ¤ relative to LC. This alteration in co-stimulatory molecule expression was selective, because UV-M ¤ express equal levels of ICAM-1 and ICAM-3, and increased levels of LFA-1, relative to LC. After bidirectional signaling with T cells during alloantigen presentation, UV-M ¤ still exhibited less CD40 and B7-1 than LC. Addition of IFN-+ induced CD40 and B7-1 expression on UV-M ¤ and restored IL-2R § expression on UV-M ¤-activated T cells but had no effect on IL-2R § on resting or LC-activated T cells. The restoration of IL-2R § expression on UV-M ¤-activated T cells by IFN-+ was inhibited (67 %, p j 0.005) by addition of neutralizing anti-CD40. Therefore, differences in co-stimulatory molecule expression, in particular CD40, on UV-M ¤ and LC are critical in determining the distinct T cell activation induced by these APC.

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Distinct cytokine use and production by T cells after activation by langerhans cells versus macrophages infiltrating UV-irradiated epidermis

Cited by 3 publications

References 0 publications

Ultraviolet B Radiation Induces a Transient Appearance of IL-4+ Neutrophils, Which Support the Development of Th2 Responses

Ultraviolet B Radiation Induces a Transient Appearance of IL-4+ Neutrophils, Which Support the Development of Th2 Responses

The effects of ultraviolet radiation on the human immune system

Low expression of CD40 and B7 on macrophages infiltrating UV-exposed human skin; role in IL-2Rα− T cell activation

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