Distinct Characteristics of Escherichia coli Isolated from Patients with Urinary Tract Infections in a Medical Center at a Ten-Year Interval

Abstract

Escherichia coli causing urinary tract infections (UTIs) are one of the most common outpatient bacterial infections. This study aimed to compare the characteristics of E. coli isolated from UTI patients in a single medical center in 2009–2010 (n = 504) and 2020 (n = 340). The antimicrobial susceptibility of E. coli was determined by the disk diffusion method. PCRs were conducted to detect phylogenetic groups, ST131, K1 capsule antigen, and 15 virulence factors. Phylogenetic group B2 dominated in our 2009–2010 and 2020 isolates. Moreover, no phylogenetic group E strains were isolated in 2020. E. coli isolates in 2020 were more susceptible to amoxicillin, ampicillin/sulbactam, cefuroxime, cefmetazole, ceftazidime, cefoxitin, tetracycline, and sulfamethoxazole/trimethoprim, compared to the isolates in 2009–2010. Extensively drug-resistant (XDR)-E. coli in 2009–2010 were detected in groups B1 (5 isolates), B2 (12 isolates), F (8 isolates), and unknown (1 isolate). In 2020, XDR-E. coli were only detected in groups A (2 isolates), B2 (5 isolates), D (1 isolate), and F (4 isolates). The prevalence of virulence factor genes aer and fimH were higher in E. coli in 2009–2010 compared to those in 2020. In contrast, afa and sat showed higher frequencies in E. coli isolates in 2020 compared to E. coli in 2009–2010.

Occurrence and Genomic Characterization of Clone ST1193 Clonotype 14-64 in Uncomplicated Urinary Tract Infections Caused by Escherichia coli in Spain

“…From the hands-on time and cost point of view, this workflow seems a reasonable approach for the surveillance of emerging clones. As reported in different studies, the phylogenetic group B2 is the predominant within UTI isolates, even though other seven E. coli phylogroups sensu stricto appear represented to a greater or lesser extent ( 18 , 33 , 37 , 38 ). Among the 59 different PG-CH combinations determined in our study, 11 E. coli clones comprised 46% of the 100 uUTI isolates.…”

Virulence-associated traits and antimicrobial resistance of the uropathogenic Escherichia coli (UPEC) strains in relation to phylogenetic background and host factors from Malaysian patients

“…Among the seven phylogroups, phylogroup B2 was the most prominent phylogroup that accounted for 63.8% of the UPEC isolates (Table 1 and Table 2). This observation resembled the previous findings in Southeast Asia countries (e.g., Thailand and Vietnam), East Asia countries (e.g., Taiwan, Mongolia and Korea) and European countries (e.g., Romania, Germany and France) (Ramos et al, 2012;Toval et al, 2014;Lavigne et al, 2016;Munkhdelger et al, 2017;Cristea et al, 2019;Tewawong et al, 2020;Hyun et al, 2021;Lin et al, 2021b).…”

“…Little research emphasised the interactions between the bacterial traits and the host factors, especially for host age (Lin et al, 2021a). Although recent studies have demonstrated that the UPEC strains isolated from elderly patients showed greater antimicrobial resistance and conferred less VGs (Lin et al, 2021a;2021b), these works were constrained to a specific geographical area. More literature is needed to clarify the relationships between the bacterial traits of the UPEC strains and the host factors.…”

Whole-genome-sequence-based characterization of an NDM-5-producing uropathogenic Escherichia coli EC1390

“…Informed consent was waived because of the retrospective nature of the study, the isolates were remnants from patient samples, and the analysis used anonymous clinical data. A total of 844 non-duplicate E. coli isolates were identified in the clinical laboratory by colony morphology, Gram stain, biochemical tests, and the Vitek system (bioMérieux, Marcy l'Etoile, France) according to the manufacturer's recommendations [ 12 ]. E. coli isolates were stored at − 80 °C in lysogeny broth (LB) containing 20% glycerol (v/v) until tested.…”