2010
DOI: 10.1016/j.jasms.2010.03.025
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Abstract: The yields of post-source decay (PSD) and time-resolved photodissociation (PD) at 193 and 266 nm were measured for singly protonated leucine enkephalin ([YGGFL ϩ H] ϩ ), a benchmark in the study of peptide ion dissociation, by using tandem time-of-flight mass spectrometry. The peptide ion was generated by matrix-assisted laser desorption ionization (MALDI) using 2,5-dihydroxybenzoic acid as the matrix. The critical energy (E 0 ) and entropy (⌬S ‡ at 1000 K) for the dissociation were determined by Rice-Ramsperg… Show more

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Cited by 13 publications
(22 citation statements)
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References 36 publications
(75 reference statements)
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“…13 In a typical MALDI-TOF instrument, the flight time of a precursor ion from the ion source to the reflectron entrance is on the order of 10 µs. 19 Hence, this is the time scale for dissociation reactions observed by PSD. PSD spectra for peptide ions are almost completely dominated by b, y, and their consecutive dissociation products such as a (b -CO) and z (y -NH 3 ).…”
Section: 10mentioning
confidence: 98%
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“…13 In a typical MALDI-TOF instrument, the flight time of a precursor ion from the ion source to the reflectron entrance is on the order of 10 µs. 19 Hence, this is the time scale for dissociation reactions observed by PSD. PSD spectra for peptide ions are almost completely dominated by b, y, and their consecutive dissociation products such as a (b -CO) and z (y -NH 3 ).…”
Section: 10mentioning
confidence: 98%
“…To find an explanation for this interesting observation, we estimated the effective temperatures of the peptide ion by kinetic analysis of its dissociation yields in ISD and PSD. 19,21 The temperatures of the early and late CHCA plumes thus estimated were 874 and 461 K, respectively. 21 A large difference in the peptide ion temperature between the early and late plumes might occur if the ion activation mechanisms responsible for ISD and PSD are different, such as the exothermicity in proton transfer 17 for ISD and low-energy collisional activation for PSD.…”
mentioning
confidence: 93%
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“…In contrast, the absorption of UV photons leads to the excitation of ions to higher electronic states and access to high-energy dissociation pathways not observed for CID or IRMPD. Photodissociation has been implemented in MALDI time-of-flight mass spectrometers for the analysis of singly charged ions [14][15][16][17][18][19][20][21][22][23] and ESI ion trapping instruments (both FTICR systems [24][25][26][27] and various quadrupole ion traps ) for the characterization of singly or multiply charged species. In general, time-of-flight instruments offer the advantages of high resolution, high accuracy, broad m/z range, and fast scan times, although photodissociation efficiencies in these mass spectrometers have been rather low due to the fact that irradiation of the ions occurs orthogonally in a very narrow time window as the selected ions exit the ion gating region.…”
mentioning
confidence: 99%
“…A variety of lasers have been used for photodissociation, including CO 2 lasers (10.6 μm, 0.12 eV per photon) [24-27, 40-46, 48-52, 54-56, 61-63], F 2 excimer lasers (157 nm, 7.9 eV per photon) [14][15][16][17][18][19][20][21][22][23]39], ArF excimers (193 nm, 6.4 eV per photon) [57][58][59], Nd:YAG lasers (266 nm, 4.7 eV per photon [28][29][30][31][32], or 355 nm, 3.5 eV per photon [47,49]), femtosecond titanium sapphire lasers (800 nm, 1.5 eV per photon) [60], and OPO-Nd:YAG lasers that offer a tunable range from 205 nm to 2550 nm (6.0-0.49 eV per photon) [33][34][35][36][37]. As long as the laser provides sufficient power and offers a wavelength that will be absorbed by the analyte ions of interest, then it can be utilized for photodissociation.…”
mentioning
confidence: 99%