DISSIMILATION OF GLUCOSE AT CONTROLLED pH VALUES BY PIGMENTED AND NON-PIGMENTED STRAINS OF
            <i>ESCHERICHIA COLI</i>

Blackwood, A. C.; Ledingham, G. A.; Neish, A. C.

doi:10.1128/jb.72.4.497-499.1956

Cited by 80 publications

(17 citation statements)

References 10 publications

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“…This behavior suggests that the enzyme formate-hydrogen lyase may be activated under these experimental conditions (Gottschalk, 1986). The same phenomenon correlating pH and formic acid catabolism was reported by Blackwood et al (1956) and Belaich and Belaich (1976) for E. coli. The above-mentioned phenomenon was not observed in the cell suspension obtained from aerobic cultures, though formic acid secretion took place.…”

Section: Resultssupporting

confidence: 79%

Comparative Studies of Glucose Catabolism by Escherichia coli Grown in a Complex Medium under Aerobic and Anaerobic Conditions

Ricci

Hitzmann

Rinas

et al. 1990

Biotechnology Progress

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Escherichia coli HBlOl was grown in complex medium under anaerobic and aerobic conditions. Cells prepared under these two different conditions were characterized by two-dimensional protein gel electrophoresis, by NMR measurements under identical (anaerobic) conditions, and by measuring the kinetics of glucose uptake and catabolite end-product appearance in the medium under identical anaerobic conditions. Specific rates of glucose uptake and end-product formation were significantly greater for the anaerobically grown cells, which also exhibited lower intracellular concentrations of sugar phosphates, nucleoside di-and triphosphates, UDPG, and NAD(H). Two-dimensional gel electrophoretic analyses reveal changes in the intracellular levels of proteins involved in pyruvate catabolism that have been observed previously for E. coli grown in minimal medium under aerobic and anaerobic conditions. Enzymes involved in the TCA cycle were not detected in cells grown aerobically or anaerobically in complex medium.

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Section: Resultssupporting

confidence: 79%

Comparative Studies of Glucose Catabolism by Escherichia coli Grown in a Complex Medium under Aerobic and Anaerobic Conditions

Ricci

Hitzmann

Rinas

et al. 1990

Biotechnology Progress

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“…Second, the near equimolar production of both acetate and ethanol predicted by MS4 is supported by experimental data. A sampling of literature shows that acetate and ethanol production levels during anaerobic growth are usually quite similar (Alam and Clark, 1989;Belaich and Belaich, 1976;Blackwood et al, 1956;Chesbro et al, 1979). Like MS3, MS4 requires the activity of PFL.…”

Section: Observed Regulation Patterns Are Consistent With Identified mentioning

confidence: 99%

Fundamental Escherichia coli biochemical pathways for biomass and energy production: Identification of reactions

Carlson

Srienc

2003

Biotech & Bioengineering

104

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Cells grow by oxidizing nutrients using a complex network of biochemical reactions. During this process new biological material is produced along with energy used for maintaining cellular organization. Because the metabolic network is highly branched, these tasks can be accomplished using a wide variety of unique reaction sequences. However, evolutionary pressures under carbon-limited growth conditions likely select organisms that utilize highly efficient pathways. Using elementary-mode analysis, we demonstrate that the metabolism of the bacterium Escherichia coli contains four unique pathways that most efficiently convert glucose and oxygen into new cells and maintenance energy under any level of oxygen limitation. Observed regulatory patterns and experimental findings suggest growing cells use these highly efficient pathways. It is predicted that five knockout mutations generate a strain that supports growth using only the most efficient reaction sequence. The analysis approach should be generally useful for predicting metabolic capabilities and efficient network designs based on only genomic information.

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“…In air, such acid induction does not occur [20]. The consequence of this is that the proportion of lactate relative to acetate/ethanol/formate is higher the more acidic the medium during anaerobic fermentation [3,[15][16][17]. In effect the cell is replacing two formic acid, one acetic acid and one ethanol with two molecules of lactic acid (see Fig.…”

Section: Lactic Acidmentioning

confidence: 99%

“…The acetyl-CoA produced by PFL may be converted to acetate or ethanol (see below) and the formic acid may be broken down further to give hydrogen and carbon dioxide, by formate hydrogen lyase (FHL) [2]. Consideration of FHL is beyond the scope of this review, except to note that FHL is induced anaerobically both by its substrate formate and also by increased acidity [16,55,56]. In this last property, it resembles the fermentative LDH (see section 7).…”

Section: Formic Acid and Pyruvate For-mate Lyasementioning

confidence: 99%

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The fermentation pathways of Escherichia coli

Clark¹

1989

FEMS Microbiology Reviews

351

240

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Under anaerobic conditions and in the absence of alternative electron acceptors Escherichia coli converts sugars to a mixture of products by fermentation. The major soluble products are acetate, ethanol, acetate and formate with smaller amounts of succinate. In addition the gaseous products hydrogen and carbon dioxide are produced in substantial amounts. The pathway generating fermentation products is branched and the flow down each branch is varied in response both to the pH of the culture medium and the nature of the fermentation substrate. In particular, the ratio of the various fermentation products is manipulated in order to balance the number of reducing equivalents generated during glycolytic breakdown of the substrate. The enzymes and corresponding genes involved in these fermentation pathways are described. The regulatory responses of these genes and enzymes are known but the details of the underlying regulatory mechanisms are still obscure.

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DISSIMILATION OF GLUCOSE AT CONTROLLED pH VALUES BY PIGMENTED AND NON-PIGMENTED STRAINS OF ESCHERICHIA COLI

Cited by 80 publications

References 10 publications

Comparative Studies of Glucose Catabolism by Escherichia coli Grown in a Complex Medium under Aerobic and Anaerobic Conditions

Comparative Studies of Glucose Catabolism by Escherichia coli Grown in a Complex Medium under Aerobic and Anaerobic Conditions

Fundamental Escherichia coli biochemical pathways for biomass and energy production: Identification of reactions

The fermentation pathways of Escherichia coli

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