Dissection of genetic architecture for glucosinolate accumulations in leaves and seeds of Brassica napus by genome‐wide association study

Abstract

Glucosinolates (GSLs), whose degradation products have been shown to be increasingly important for human health and plant defence, compose important secondary metabolites found in the order Brassicales. It is highly desired to enhance pest and disease resistance by increasing the leaf GSL content while keeping the content low in seeds of Brassica napus, one of the most important oil crops worldwide. Little is known about the regulation of GSL accumulation in the leaves. We quantified the levels of 9 different GSLs and 15 related traits in the leaves of 366 accessions and found that the seed and leaf GSL content were highly correlated (r = 0.79). A total of 78 loci were associated with GSL traits, and five common and eleven tissue-specific associated loci were related to total leaf and seed GSL content. Thirty-six candidate genes were inferred to be involved in GSL biosynthesis. The candidate gene BnaA03g40190D (BnaA3.MYB28) was validated by DNA polymorphisms and gene expression analysis. This gene was responsible for high leaf/low seed GSL content and could explain 30.62% of the total leaf GSL variation in the low seed GSL panel and was not fixed during double-low rapeseed breeding. Our results provide new insights into the genetic basis of GSL variation in leaves and seeds and may facilitate the metabolic engineering of GSLs and the breeding of high leaf/low seed GSL content in B. napus. 1472

An integrated peach genome structural variation map uncovers genes associated with fruit traits

“…There is considerable evidence that SVs control many phenotypic traits [28,36,37], and GWAS represents a powerful tool for mapping genes in genetic and molecular biology studies [19,34,35,38,39]. Due to the reliable detection of SNPs, GWAS using SNPs has been widely used; however, there are only a few studies showing that a single SNP can change gene function and the phenotype.…”

“…4d). It has been reported that the expression of PpMYB10.1 is regulated by the BL transcription factor [38]; however, the effect of the deletion on the promoter activity of PpMYB10.1 remains unknown. In a dual-luciferase reporter assay, we found that the deletion enhanced the promoter activity, consistent with a role of PpMYB10.1 in flesh color formation around the stone (Fig.…”

Glucosinolates: Natural Occurrence, Biosynthesis, Accessibility, Isolation, Structures, and Biological Activities

“…GSL concentration is unequally distributed throughout the plant body. For instance, in Brassica napus , the GSL concentration in the seed is greater than that in leaves [ 22 ]. This variation appears to be more relevant in root vegetable crops ( Moringacea family) than that in oilseed crops ( Brassicaceae family).…”

Genetic Characterization of Russian Rapeseed Collection and Association Mapping of Novel Loci Affecting Glucosinolate Content

“…In order to compare the newly identified loci (SNP positions) with the loci that were previously demonstrated to be significantly associated with glucosinolate content, first, the available information on the exact physical distance of loci associated with glucosinolate content was collected from the previously published papers [24][25][26]42,43]. Second, for each SNP in the present study, the corresponding positions in the Darmor bzh assembly version 4.1 [3] were found.…”

“…These studies identified a number of genetic loci associated with glucosinolate content located on the chromosomes A01, A04, A06, A09, C02, C03, and C09. Following the increasing popularity of GWAS several works have been published on implementing this method to diverse rapeseed lines from global collections [24][25][26]. These approaches facilitated the identification of new loci as well as new candidate genes potentially responsible for glucosinolate content in rapeseed plants.…”