Dissecting sensor functions in cell wall integrity signaling in Kluyveromyces lactis

Rodicio, Rosaura; Buchwald, Ulf; Schmitz, Hans‐Peter; Heinisch, Jürgen J.

doi:10.1016/j.fgb.2007.07.009

Cited by 49 publications

(44 citation statements)

References 45 publications

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“…However, strains carrying this sensor show only a complementation of the mid2-, but not of the wsc1-specific growth defects, indicating that the membrane distribution of a sensor may not be a primary determinant of its specificity (Wilk, Wittland, Thywissen, Schmitz and Heinisch, manuscript submitted). In K. lactis, a similar behaviour was observed with GFP fusions of the homologues KlWsc1 and KlMid2 [45]. Interestingly, KlWsc1 contains a NPFAD signal in its C-terminal tail (positions 393-397), which is not present in either KlWsc2/3 or KlMid2.…”

Section: Sensor Distribution and Dynamicssupporting

confidence: 59%

“…As also indicated in Figure 2, three homologous sensors could be identified in the milk yeast K. lactis (KlWsc1, KlWsc2/3 and KlMid2), where they ensure cell integrity [45]. It should be noted that the cell wall of K. lactis is considerably thinner than that of S. cerevisiae for cells growing on glucose [1], indicating that the head groups of the sensors will be closer to the surface than those of their homologues in S. cerevisiae.…”

Section: Sensor Structure and Mechanicsmentioning

confidence: 82%

“…The ability of hybrid sensors (which had their extracellular regions exchanged between Wsc1 and Mid2) to complement the various phenotypes of a wsc1 mid2 double deletion strain indicated that a large part of the specific responses to extracellular stresses is mediated by the cytoplasmic tail [51]. On the other hand, heterologous complementation studies of the sensors from K. lactis in S. cerevisiae, and vice versa, showed that the extracellular regions are responsible for species specificity [45].…”

Section: Sensor Distribution and Dynamicsmentioning

confidence: 99%

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Together we are strong—cell wall integrity sensors in yeasts

Rodicio

Heinisch

2010

Yeast

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105

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The integrity of the fungal cell wall is ensured by a signal transduction pathway, the so-called CWI pathway, which has best been studied in the model yeast Saccharomyces cerevisiae. In this context, environmental stress and other perturbations at the cell surface are detected by a small set of plasma membrane-spanning sensors, viz. Wsc1, Wsc2, Wsc3, Mid2 and Mtl1. This review covers the recent advances in sensor structure, sensor mechanics, their cellular distribution and their in vivo functions, obtained from genetic, biochemical, cell biological and biophysical investigations.

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Section: Sensor Distribution and Dynamicssupporting

confidence: 59%

Section: Sensor Structure and Mechanicsmentioning

confidence: 82%

Section: Sensor Distribution and Dynamicsmentioning

confidence: 99%

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Together we are strong—cell wall integrity sensors in yeasts

Rodicio

Heinisch

2010

Yeast

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105

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“…1). The CWI pathway encompasses several stress sensors at the cell surface, Rho GTPases, protein kinase C, mitogen-activated protein (MAP) kinases, phosphatases, and other components, and in yeasts, this pathway appears to be roughly conserved (2)(3)(4). Cell wall stress is sensed at the cell surface by certain stress sensors.…”

mentioning

confidence: 99%

Role of the Guanine Nucleotide Exchange Factor Rom2 in Cell Wall Integrity Maintenance of Aspergillus fumigatus

et al. 2013

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Aspergillus fumigatus is a mold and the causal agent of invasive aspergillosis, a systemic disease with high lethality. Recently, we identified and functionally characterized three stress sensors implicated in the cell wall integrity (CWI) signaling of this pathogen, namely, Wsc1, Wsc3, and MidA. Here, we functionally characterize Rom2, a guanine nucleotide exchange factor with essential function for the cell wall integrity of A. fumigatus. A conditional rom2 mutant has severe growth defects under repressive conditions and incorporates all phenotypes of the three cell wall integrity sensor mutants, e.g., the echinocandin sensitivity of the ⌬wsc1 mutant and the Congo red, calcofluor white, and heat sensitivity of the ⌬midA mutant. Rom2 interacts with Rho1 and shows a similar intracellular distribution focused at the hyphal tips. Our results place Rom2 between the cell surface stress sensors Wsc1, Wsc3, MidA, and Rho1 and their downstream effector mitogen-activated protein (MAP) kinase module Bck1-Mkk2-MpkA.

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“…Moreover, the CWI sensor Wsc1 accumulates in the growing bud and, later on, at the bud neck in both S. cerevisiae and K. lactis (Rodicio et al , 2008 ;Wilk et al , 2010 ). There are some striking coincidences related to the mechanism of localization of these components: i) Rho1 is apparently recruited either by its interaction with the GEF Rom2 or with PI(4,5)P 2 , with the latter also recruiting Pil1 and Lsp1, the main eisosome proteins (Yoshida et al , 2009 ;Karotki et al , 2011 ).…”

mentioning

confidence: 99%

Microcompartments within the yeast plasma membrane

Merzendorfer

Heinisch

2013

Biological Chemistry

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Recent research in cell biology makes it increasingly clear that the classical concept of compartmentation of eukaryotic cells into different organelles performing distinct functions has to be extended by microcompartmentation, i.e., the dynamic interaction of proteins, sugars, and lipids at a suborganellar level, which contributes significantly to a proper physiology. As different membrane compartments (MCs) have been described in the yeast plasma membrane, such as those defined by Can1 and Pma1 (MCCs and MCPs), Saccharomyces cerevisiae can serve as a model organism, which is amenable to genetic, biochemical, and microscopic studies. In this review, we compare the specialized microcompartment of the yeast bud neck with other plasma membrane substructures, focusing on eisosomes, cell wall integrity-sensing units, and chitin-synthesizing complexes. Together, they ensure a proper cell division at the end of mitosis, an intricately regulated process, which is essential for the survival and proliferation not only of fungal, but of all eukaryotic cells.

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Dissecting sensor functions in cell wall integrity signaling in Kluyveromyces lactis

Cited by 49 publications

References 45 publications

Together we are strong—cell wall integrity sensors in yeasts

Together we are strong—cell wall integrity sensors in yeasts

Role of the Guanine Nucleotide Exchange Factor Rom2 in Cell Wall Integrity Maintenance of Aspergillus fumigatus

Microcompartments within the yeast plasma membrane

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