Disruption of Gene pqqA or pqqB Reduces Plant Growth Promotion Activity and Biocontrol of Crown Gall Disease by Rahnella aquatilis HX2

Li, Lei; Jiao, Ziwei; Hale, Lauren; Wu, Wenliang; Guo, Yanbin

doi:10.1371/journal.pone.0115010

Cited by 40 publications

(32 citation statements)

References 48 publications

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“… 52 . The mineral P-solubilizing ability of LTYR-11Z T was first assessed on NBRIP agar plates containing 0.5% Ca 3 (PO 4 ) 2 53 . Quantitative estimation of phosphate solubilization was performed in NBRIP liquid medium using the Molybdenum-blue method 54 .…”

Section: Methodsmentioning

confidence: 99%

Pantoea alhagi, a novel endophytic bacterium with ability to improve growth and drought tolerance in wheat

Chen

Xin

Líu

et al. 2017

Sci Rep

148

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A novel strain LTYR-11ZT that exhibited multiple plant growth promoting (PGP) traits was isolated from the surface-sterilized leaves of Alhagi sparsifolia Shap. (Leguminosae), which reprsents one of the top drought tolerant plants in north-west China. Phylogenetic analysis of 16S rRNA gene sequences and multilocus sequence analysis based on partial sequences of atpD, gyrB, infB and rpoB genes revealed that strain LTYR-11ZT was a member of the genus Pantoea, with Pantoea theicola NBRC 110557T and Pantoea intestinalis DSM 28113T as the closest phylogenetic relatives. The results of DNA–DNA hybridization, phenotypic tests and fatty acid analysis confirmed that strain LTYR-11ZT represents a novel species of the genus Pantoea, for which we propose the name Pantoea alhagi sp. nov. Confocal microscopy observation revealed that strain LTYR-11ZT effectively colonizes the rhizoplane of both Arabidopsis and wheat. Strain LTYR-11ZT was able to promote the growth of wheat enhancing its resistance to drought stress. Strain LTYR-11ZT led to increased accumulation of soluble sugars, decreased accumulation of proline and malondialdehyde (MDA), and decreased degradation of chlorophyll in leaves of drought-stressed wheat. Our findings will contribute to the development of a novel biotechnological agent to improve the adaptation of crop plants to drought in arid ecosystems.

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Section: Methodsmentioning

confidence: 99%

Pantoea alhagi, a novel endophytic bacterium with ability to improve growth and drought tolerance in wheat

Chen

Xin

Líu

et al. 2017

Sci Rep

148

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“…Three of these pqqECB genes were found to be up-regulated in Free Redstar. PQQ is known to be a small, redox-active molecule that serves as a cofactor for several bacterial dehydrogenases, introducing pathways for carbon utilization that confer a growth advantage, but it was also shown to be essential for antibacterial activity of the biocontrol agent Rahnella aquatilis [ 46 ].…”

Section: Resultsmentioning

confidence: 99%

Comparative transcriptome analysis of a lowly virulent strain of Erwinia amylovora in shoots of two apple cultivars – susceptible and resistant to fire blight

et al. 2017

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Background Erwinia amylovora is generally considered to be a homogeneous species in terms of phenotypic and genetic features. However, strains show variation in their virulence, particularly on hosts with different susceptibility to fire blight. We applied the RNA-seq technique to elucidate transcriptome-level changes of the lowly virulent E. amylovora 650 strain during infection of shoots of susceptible (Idared) and resistant (Free Redstar) apple cultivars.ResultsThe highest number of differentially expressed E. amylovora genes between the two apple genotypes was observed at 24 h after inoculation. Six days after inoculation, only a few bacterial genes were differentially expressed in the susceptible and resistant apple cultivars. The analysis of differentially expressed gene functions showed that generally, higher expression of genes related to stress response and defence against toxic compounds was observed in Free Redstar. Also in this cultivar, higher expression of flagellar genes (FlaI), which are recognized as PAMP (pathogen-associated molecular pattern) by the innate immune systems of plants, was noted. Additionally, several genes that have not yet been proven to play a role in the pathogenic abilities of E. amylovora were found to be differentially expressed in the two apple cultivars.ConclusionsThis RNA-seq analysis generated a novel dataset describing the transcriptional response of the lowly virulent strain of E. amylovora in susceptible and resistant apple cultivar. Most genes were regulated in the same way in both apple cultivars, but there were also some cultivar-specific responses suggesting that the environment in Free Redstar is more stressful for bacteria what can be the reason of their inability to infect of this cultivar. Among genes with the highest fold change in expression between experimental combinations or with the highest transcript abundance, there are many genes without ascribed functions, which have never been tested for their role in pathogenicity. Overall, this study provides the first transcriptional profile by RNA-seq of E. amylovora during infection of a host plant and insights into the transcriptional response of this pathogen in the environments of susceptible and resistant apple plants.Electronic supplementary materialThe online version of this article (10.1186/s12864-017-4251-z) contains supplementary material, which is available to authorized users.

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“…; Li et al . ); therefore, R. aquatilis has been extensively studied for agricultural and industrial applications. Recently, a few clinical isolates of R. aquatilis have been found to be associated with human infections and resistant to antimicrobial agents (Martins et al .…”

Section: Introductionmentioning

confidence: 99%

Identification ofatpDas an optimal reference gene to explore antibiotic resistance and stress tolerance inRahnella aquatilis

Peng

et al. 2019

J Appl Microbiol

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Aims: Rahnella aquatilis is a Gram-negative bacterial species with potential for agricultural and industrial applications, as well as a human pathogen. This study aims to identify an optimal reference gene to explore antibiotic resistance and stress tolerance in R. aquatilis using reverse-transcription quantitative polymerase chain reaction (RT-qPCR). Methods and Results: Expression levels of 14 housekeeping genes in R. aquatilis were estimated by RT-qPCR under six different conditions: exponential phase, stationary phase, acid, salinity, antibiotic and oxidative stresses. BestKeeper and the DCt method were used to evaluate the stability of each gene. The atpD gene was stably expressed in all conditions, thus was selected and validated as an optimal reference gene. Transcript levels of 17 putative ampicillin-resistance genes in R. aquatilis strain HX2 were evaluated using the proposed RT-qPCR. Six genes encoding efflux transporters and b-lactamase were overexpressed after ampicillin treatment. Additionally, the expression of seven putative stress response genes in strain HX2 was assessed, and five genes were up-regulated by respective stress treatments. Conclusions: The atpD gene has been identified as an optimal reference gene for expression analysis of R. aquatilis responses to abiotic stresses by RT-qPCR. Significance and Impact of the Study: The proposed RT-qPCR is suitable for gene expression analysis in R. aquatilis, thus useful for studying antimicrobial resistance and stress tolerance in this bacterium and others closely related.

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Disruption of Gene pqqA or pqqB Reduces Plant Growth Promotion Activity and Biocontrol of Crown Gall Disease by Rahnella aquatilis HX2

Cited by 40 publications

References 48 publications

Pantoea alhagi, a novel endophytic bacterium with ability to improve growth and drought tolerance in wheat

Pantoea alhagi, a novel endophytic bacterium with ability to improve growth and drought tolerance in wheat

Comparative transcriptome analysis of a lowly virulent strain of Erwinia amylovora in shoots of two apple cultivars – susceptible and resistant to fire blight

Identification ofatpDas an optimal reference gene to explore antibiotic resistance and stress tolerance inRahnella aquatilis

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