Disruption of cytochrome P450 enzymes in the liver and small intestine in chicken embryos in ovo exposed to glyphosate

Fathi, Mohamed; Han, Guofeng; Kang, Ruifen; Shen, Dan; Shen, Jieliang; Li, Chunmei

doi:10.1007/s11356-020-08269-3

Cited by 25 publications

(17 citation statements)

References 59 publications

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“…Some villi appeared with short atrophy, whereas others showed degeneration and were blunt. These characteristics are in agreement with those reported by Fathi et al (2020), who observed that the size and height of the villus were lower among different parts of the small intestine of chicken embryo treated with glyphosate. In teleost fish Channa punctatus, the toxic effect of Elsan organophosphate pesticide resulted in disruption of the villi structure (Kosik-Bogacka & Kolasa, 2012).…”

Section: Discussionsupporting

confidence: 93%

Impact of Glyphosate-Roundup® in the Ileal Structure of Male and Female Rats: A Morphological and Immunohistochemical Study

Saleh

Elghareeb

Atia

et al. 2021

Microscopy and Microanalysis

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The current study was aimed to evaluate the effects of variable doses of the weedicide glyphosate on the ileal (the final section of the small intestine) structure of rats of both sexes, using histological, histochemical, and ultrastructural methods. Forty animals were classified into four groups of 10 animals per group (five males and five females). The first group acted as a control, and the remaining groups were treated with glyphosate-Roundup® 25, 50, and 100 mg/kg body weight daily for 15 days. The results indicated extinct histopathological changes manifested in the deformation of villi, foci of leukocytic infiltration in the core of villi, and hyperplasia of goblet cells. Histochemical examination (Alcian blue and Periodic acid–Schiff stain) revealed a strong positive reaction of goblet cells and an increase in their number in all treated groups. In addition, the immunohistochemical investigation revealed the immunoreactivity of matrix metalloproteinase-9 expression. Furthermore, electron microscopic alternations were represented by the deformation of nuclei, destruction of microvilli, and deposition of lipid droplets. Collectively, the present findings indicate that treatment with glyphosate results in extensive morphological alternations to the ileal structure of rats of both sexes and that female rats are more affected than male rats are.

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Section: Discussionsupporting

confidence: 93%

Impact of Glyphosate-Roundup® in the Ileal Structure of Male and Female Rats: A Morphological and Immunohistochemical Study

Saleh

Elghareeb

Atia

et al. 2021

Microscopy and Microanalysis

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“…However, there was a decrease in the hatching rate; an increase in kidney and liver weights and oxidative stress; serum parameter imbalances; histopathological alterations of the kidney, liver, and small intestine; and an increase in the levels of liver function enzymes. Moreover, some perturbations of cytochrome P450 family enzymes were recorded [85,86]. However, embryos exposed from the first day of incubation had a decreased bodyweight with another GBH: Fozat 480 at a higher dose (9.6 g/L).…”

Section: In Vitro Studiesmentioning

confidence: 99%

Review: Mechanisms of Glyphosate and Glyphosate-Based Herbicides Action in Female and Male Fertility in Humans and Animal Models

Serra

Estienne

Vasseur³

et al. 2021

Cells

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Glyphosate (G), also known as N-(phosphonomethyl)glycine is the declared active ingredient of glyphosate-based herbicides (GBHs) such as Roundup largely used in conventional agriculture. It is always used mixed with formulants. G acts in particular on the shikimate pathway, which exists in bacteria, for aromatic amino acids synthesis, but this pathway does not exist in vertebrates. In recent decades, researchers have shown by using various animal models that GBHs are endocrine disruptors that might alter reproductive functions. Our review describes the effects of exposure to G or GBHs on the hypothalamic–pituitary–gonadal (HPG) axis in males and females in terms of endocrine disruption, cell viability, and proliferation. Most of the main regulators of the reproductive axis (GPR54, GnRH, LH, FSH, estradiol, testosterone) are altered at all levels of the HPG axis (hypothalamus, pituitary, ovaries, testis, placenta, uterus) by exposure to GBHs which are considered more toxic than G alone due to the presence of formulants such as polyoxyethylene tallow amine (POEA).” In addition, we report intergenerational impacts of exposure to G or GBHs and, finally, we discuss different strategies to reduce the negative effects of GBHs on fertility.

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“…Here, we show for the first time that chicks from dietary RU-exposed fathers had a higher food intake, body weight and average daily gain during the first 10 days. This was not observed in in ovo studies in chickens [24,25], and the opposite was observed in in vivo studies in the F1 generation [14,57] or the F2 generation [58]. In the literature, the opposite effect was also observed in F1 adult mice [59] when they were pre-and postnatally exposed to GBH.…”

Section: Discussionmentioning

confidence: 81%

“…In adult drakes (Anas platyrhynchos), exposure to GBHs for 15 days caused a reduced level of plasma T and E2 as well as a reduction in the epithelium of the seminiferous tubules (ST) and interstitial tissue, leading to an altered epididymis morphology [22]. Finally, studies focusing on the in ovo exposure of chicken embryos found that GBHs promoted embryonic mortality [23], reduced the percentage of hatching [24], disrupted cytochrome P450 enzymes in the liver and small intestine [25] and increased reactive oxygen species (ROS) production [24,25]. Only a few studies have been performed on the effects of GBHs in avian species.…”

Section: Introductionmentioning

confidence: 99%

Chronic Dietary Exposure of Roosters to a Glyphosate-Based Herbicide Increases Seminal Plasma Glyphosate and AMPA Concentrations, Alters Sperm Parameters, and Induces Metabolic Disorders in the Progeny

Serra

Estienne

Bourdon

et al. 2021

Toxics

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The effects of chronic dietary Roundup (RU) exposure on rooster sperm parameters, fertility, and offspring are unknown. We investigated the effects of chronic RU dietary exposure (46.8 mg kg−1 day−1 glyphosate) for 5 weeks in 32-week-old roosters (n = 5 RU-exposed and n = 5 control (CT)). Although the concentrations of glyphosate and its main metabolite AMPA (aminomethylphosphonic acid) increased in blood plasma and seminal fluid during exposure, no significant differences in testis weight and sperm concentrations were observed between RU and CT roosters. However, sperm motility was significantly reduced, associated with decreased calcium and ATP concentrations in RU spermatozoa. Plasma testosterone and oestradiol concentrations increased in RU roosters. These negative effects ceased 14 days after RU removal from the diet. Epigenetic analysis showed a global DNA hypomethylation in RU roosters. After artificial insemination of hens (n = 40) with sperm from CT or RU roosters, eggs were collected and artificially incubated. Embryo viability did not differ, but chicks from RU roosters (n = 118) had a higher food consumption, body weight and subcutaneous adipose tissue content. Chronic dietary RU exposure in roosters reduces sperm motility and increases plasma testosterone levels, growth performance, and fattening in offspring.

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Disruption of cytochrome P450 enzymes in the liver and small intestine in chicken embryos in ovo exposed to glyphosate

Cited by 25 publications

References 59 publications

Impact of Glyphosate-Roundup® in the Ileal Structure of Male and Female Rats: A Morphological and Immunohistochemical Study

Impact of Glyphosate-Roundup® in the Ileal Structure of Male and Female Rats: A Morphological and Immunohistochemical Study

Review: Mechanisms of Glyphosate and Glyphosate-Based Herbicides Action in Female and Male Fertility in Humans and Animal Models

Chronic Dietary Exposure of Roosters to a Glyphosate-Based Herbicide Increases Seminal Plasma Glyphosate and AMPA Concentrations, Alters Sperm Parameters, and Induces Metabolic Disorders in the Progeny

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