Disproportional synthesis of the adult duck's two hemoglobins during acute anemia

Bertles, John F.; Borgese, Thomas A.

doi:10.1172/jci105763

Cited by 18 publications

(3 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1 Hb I and Hb II. The proportion of the latter increases during hemolytic anemia (30). These hemoglobins contain the same subunits but differ in their a subunits [acA and a D, according to the nomenclature of Stewart et al (31)].…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Cell-Free Translation of the Globin Message within Polydisperse High-Molecular-Weight Ribonucleic Acid of Avian Erythrocytes

Ruiz-Carrillo

Beato

Schütz

et al. 1973

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Polydisperse high-molecular-weight RNA of nucleated avian erythrocytes includes sequences coding for globin chains. The RNA was extracted from immature erythrocytes of ducks and fractionated under denaturing conditions by sucrose density gradient centrifugation in 99% dimethylsulfoxide. The RNA sedimenting faster than 45 S was able to direct the synthesis of duck globins in the Krebs II ascites cell-free protein-synthesizing system. The newly synthesized globin molecules have been identified by their characteristic electrophoretic properties in polyacrylamide gels containing either urea or sodium dodecyl sulfate, and by immunoprecipitation of the released globin chains by rabbit antibodies against duck hemoglobin. In order to rule out the possibility of a contamination of the high-molecular-weight RNA with duckglobin messenger RNA tailing from the 9-1OS region, rabbit-globin messenger RNA was added to duck RNA as an internal control. No rabbit-globin messenger RNA activity could be detected in the RNA fractions sedimenting faster than 45 S. It is concluded that high-molecularweight RNAs in the nucleated erythroid cell contain sequences of globin messenger RNAs covalently attached to larger polynucleotide chains. These results support the view that polydisperse nuclear RNA is the precursor of the cytoplasmic messenger RNA fraction.Among the primary products of transcription in the nuclei of animal cells is a population of rapidly labeled RNA molecules with a DNA-like base composition (1, 2). This fraction of high-rtiolecular-weight nuclear RNA (HnRNA) is extremely polydisperse, with components ranging in sedimentation coefficients between 30 and 100 S (3, 4). Most of the newly synthesized HnRNA is quickly degraded inside the nucleus, so that only a small percentage of it is actually transported to the cytoplasm (2, 5, 6).The suggestion that the large HnRNA might be a precursor of the smaller messenger RNA (mRNA) population (6, 7) is supported by several lines of evidence. The finding that poly(A) segments of similar size exist at the 3'-ends of some HnRNA and most mRNA molecules (8-12) is consistent with a precursor-product relationship. Antimetabolites, such as 3'-deoxyadenosine, which block the addition of poly (A)

show abstract

Section: Resultsmentioning

confidence: 99%

“…This fraction of high-rtiolecular-weight nuclear RNA (HnRNA) is extremely polydisperse, with components ranging in sedimentation coefficients between 30 and 100 S (3,4). Most of the newly synthesized HnRNA is quickly degraded inside the nucleus, so that only a small percentage of it is actually transported to the cytoplasm (2,5,6).…”

mentioning

confidence: 99%

Cell-Free Translation of the Globin Message within Polydisperse High-Molecular-Weight Ribonucleic Acid of Avian Erythrocytes

Ruiz-Carrillo

Beato

Schütz

et al. 1973

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Measurement of the rate of synthesis of globin, rather than the heme moiety of hemoglobin, obviates the considerable difficulties associated with the use of iron uptake as an index of hemoglobin synthesis (Cole and Paul, 1966 ;Felicetti et al ., 1966 ;Bunn and Jandl, 1968 ;Djaldetti et al ., 1970) . Although it has been suggested that erythropoietin is involved in producing alterations in the types of hemoglobins synthesized in a variety of species (Moore et al ., 1966 ;Bertles and Borgese, 1968 ;Gabuzda et al ., 1968 ;Kraus et al ., 1968 ;Lewis et al ., 1968), there has been no direct demonstration of this effect . Direct observations in the rat (Hunter and Paul, 1969) of the effect of erythropoietin on the types of hemoglobin formed showed, as in the present case, no alteration in the type of hemoglobin synthesized.…”

Section: Thymidine-1 H Labeling Indexmentioning

confidence: 99%

Erythropoietin Effects on Fetal Mouse Erythroid Cells

Chui

Djaldetti

Marks

et al. 1971

The Journal of Cell Biology

View full text Add to dashboard Cite

The effect of the hormone, erythropoietin, on cultures of erythroblasts derived from the livers of fetal C57BL/6J mice was examined . An increase both in the content and in the rate of synthesis of normal adult mouse globin chains was detected in hormone-treated cultures . The rate of protein synthesis by individual erythroblasts does not increase in response to the hormone, whereas the absolute number of hemoglobin-synthesizing cells does increase and accounts for the observed stimulation of hemoglobin synthesis . The principal effect of erythropoietin appears to be upon the population of immature erythroid precursor cells which persists in the presence of the hormone, the cells maintaining their ability to replicate, and their capacity to differentiate into hemoglobinizing erythroblasts . In the absence of hormone, already committed erythroblasts continue their development, but erythropoiesis is not sustained .

show abstract

Vertebrate Hemoglobins

Ingermann¹

1997

Comprehensive Physiology

View full text Add to dashboard Cite

Disproportional synthesis of the adult duck's two hemoglobins during acute anemia

Cited by 18 publications

References 35 publications

Cell-Free Translation of the Globin Message within Polydisperse High-Molecular-Weight Ribonucleic Acid of Avian Erythrocytes

Cell-Free Translation of the Globin Message within Polydisperse High-Molecular-Weight Ribonucleic Acid of Avian Erythrocytes

Erythropoietin Effects on Fetal Mouse Erythroid Cells

Vertebrate Hemoglobins

Contact Info

Product

Resources

About