2020
DOI: 10.1038/s41467-020-19911-6 View full text |Buy / Rent full text
Has correction 2020-12-14Has erratum 2020-12
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Abstract: Rapid screening and low-cost diagnosis play a crucial role in choosing the correct course of intervention when dealing with highly infectious pathogens. This is especially important if the disease-causing agent has no effective treatment, such as the novel coronavirus SARS-CoV-2, and shows no or similar symptoms to other common infections. Here, we report a disposable silicon-based integrated Point-of-Need transducer (TriSilix) for real-time quantitative detection of pathogen-specific sequences of nucleic acid… Show more

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“…RT-PCR, based on conserved genetic sequences and synthetic nucleic acid technology, can detect viruses with no risk of contamination by operators or the test environment, especially for newly emerging SARS, MERS, [128] and COVID-19. [129][130][131][132][133] The vital process in RT-PCR depends on designing primers (a short nucleic acid sequence that provides a starting point for DNA synthesis) according to the sequence alignment. Good primer design can avoid the production of primer sets that produce false-positive results with measures such as the targeting of specific RV genes, the simulation of amplicon sequences and primers, the elimination of spurious primer dimers, and the optimization of the PCR process.…”
Section: Nucleic Acid Amplification-based Pocts For Coronavirusmentioning
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“…RT-PCR, based on conserved genetic sequences and synthetic nucleic acid technology, can detect viruses with no risk of contamination by operators or the test environment, especially for newly emerging SARS, MERS, [128] and COVID-19. [129][130][131][132][133] The vital process in RT-PCR depends on designing primers (a short nucleic acid sequence that provides a starting point for DNA synthesis) according to the sequence alignment. Good primer design can avoid the production of primer sets that produce false-positive results with measures such as the targeting of specific RV genes, the simulation of amplicon sequences and primers, the elimination of spurious primer dimers, and the optimization of the PCR process.…”
Section: Nucleic Acid Amplification-based Pocts For Coronavirusmentioning
“…And there is always a great time stability and no need for large energy consumption. A silicon-based integrated transducer (TriSilix) was devised for pathogen detection [168 ] . In detail, a large number of amplicons were produced using RPA or PCR when the target nucleic acid was present.…”
Section: Construction Of Biosensing Systems For Poct Molecular Diagnosismentioning
“…In addition to functional materials, some researchers pursue special structure design to achieve improved biosensing performance, which embodies in the entire process or sub-procedures. Sandwich structure is an effective design to simplify the workflow and minimize the space of biosensing devices, and has been introduced in many scenarios [15] , [153] , [168] , [175] , [176] , [177] . Some attempts have been made to modify the conventional molecular diagnosis platform like LFA or microfluidics by introducing additional components [178] , [179] , [180] .…”
Section: Construction Of Biosensing Systems For Poct Molecular Diagnosismentioning
“…The symptoms of the patients with the infection of COVID-19 are nonspecific, so various diagnostic methods have been developed accordingly [ 12 17 ]. To rapidly screen the subject infected with COVID-19, nucleic acid testing (NAT) based on the reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is now applied as a clinical-acceptable standard [ 18 , 19 ].…”
Section: Introductionmentioning