Disparity between Multilocus Enzyme Electrophoresis, Microsatellite Markers and Pulsed-Field Gel Electrophoresis in epidemiological tracking of Candida albicans

Boriollo, Marcelo Fabiano Gomes; Dias, Ricardo Antunes; Fiorini, João Evangelista; Oliveira, Nelma de Mello Silva; Spolidório, Denise Madalena Palomari; Souza, Henrique Marques Barbosa de; Figueira, Antônio; Pizzirani-Kleiner, Aline Aparecida

doi:10.1016/j.mimet.2010.06.012

Cited by 8 publications

(21 citation statements)

References 72 publications

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“…Indeed, when compared with other typing methods for their capacities to assess microevolution within a strain, MLEE showed a modest degree of resolution power (Boriollo et al . ). Finally, MLEE is time‐consuming because it requires the combination of results from at least 10 enzymes to provide reliable typing data and detection of variability among isolates (Boriollo et al .…”

Section: Non‐dna‐based Typing Methodsmentioning

confidence: 97%

“…Finally, MLEE is time‐consuming because it requires the combination of results from at least 10 enzymes to provide reliable typing data and detection of variability among isolates (Boriollo et al . , ).…”

Section: Non‐dna‐based Typing Methodsmentioning

confidence: 99%

“…In addition, it may not be sensitive enough in measuring rapid microevolutionary changes and, thus, may not be an adequate method for revealing substrain shuffling or microevolution in an infecting population over time. Indeed, when compared with other typing methods for their capacities to assess microevolution within a strain, MLEE showed a modest degree of resolution power (Boriollo et al 2010). Finally, MLEE is time-consuming because it requires the combination of results from at least 10 enzymes to provide reliable typing data and detection of variability among isolates (Boriollo et al 2006(Boriollo et al , 2010.…”

Section: Non-dna-based Typing Methodsmentioning

confidence: 99%

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Molecular methods for strain typing of Candida albicans : a review

Saghrouni¹,

Abdeljelil²,

Boukadida³

et al. 2013

J Appl Microbiol

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SummaryCandida albicans is one of the most medically important fungi because of its high frequency as a commensal and pathogenic microorganism causing superficial as well as invasive infections. Strain typing and delineation of the species are essential for understanding its biology, epidemiology and population structure. A wide range of molecular techniques have been used for this purpose including non-DNA-based methods (multi-locus enzyme electrophoresis), conventional DNA-based methods (electrophoretic karyotyping, random amplified polymorphic DNA, amplified fragment length polymorphism, restriction enzyme analysis with and without hybridization, rep-PCR) and DNA-based methods called exact typing methods because they generate unambiguous and highly reproducible typing data (including microsatellite length polymorphism and multi-locus sequence typing). In this review, the main molecular methods used for C. albicans strain typing are summarized, and their advantages and limitations are discussed with regard to their discriminatory power, reproducibility, cost and ease of performance.

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Section: Non‐dna‐based Typing Methodsmentioning

confidence: 97%

Section: Non‐dna‐based Typing Methodsmentioning

confidence: 99%

Section: Non-dna-based Typing Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Molecular methods for strain typing of Candida albicans : a review

Saghrouni¹,

Abdeljelil²,

Boukadida³

et al. 2013

J Appl Microbiol

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“…Phenotypic methods (biotyping, serotyping, bacteriophage or bacteriocin typing and antimicrobial susceptibility profiles) and genotypic [pulsed-field gel electrophoresis (PFGE) and other methods based on the restriction of genomes, analysis of plasmids, typing methods based on polymerase chain reaction (PCR)] of microbiological characterization have elucidated the relationship and the distribution of human pathogens, which is considered essentially important for the epidemiology and control of hospital infections 17 . Isoenzymatic typing [multilocus enzyme electrophoresis (MLEE)] has been used for several decades as a "gold standard" in population genetics studies of eukaryotes [18][19][20] and systematic studies 21 , as well as in large-scale studies for determining the genetic diversity and structure of natural populations of a variety of bacteria species [22][23][24] and fungi [25][26][27] . This method represents an invaluable complement to the more recently developed molecular typing methods, particularly for largescale epidemiological studies 28 .…”

Section: Introductionmentioning

confidence: 99%

“…This method represents an invaluable complement to the more recently developed molecular typing methods, particularly for largescale epidemiological studies 28 . In addition, MLEE possesses excellent typability (i.e., the percentage of different strains obtained) and reproducibility (i.e., the percentage of strains that display the same results in repetitive tests) and is associated with great discriminatory power (i.e., the ability to differentiate unrelated strains) [23][24][25][26][27][28][29][30][31][32][33] .…”

Section: Introductionmentioning

confidence: 99%

Isoenzyme genotyping and phylogenetic analysis of oxacillin-resistance Staphylococcus aureus isolates

Boriollo

Netto

Silva³

et al. 2017

Braz. J. Oral Sci.

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Aim: The propagation of S. aureus in hospital and dental environments is considered an important public health problem since resistant strains can cause serious infections in humans. The genetic variability of 99 oxacillin-resistant S. aureus isolates (ORSA) from the dental patients (oral cavity) and environments (air) was studied by isoenzyme genotyping. Methods: S. aureus isolates were studied using isoenzyme markers (alcohol dehydrogenase, sorbitol dehydrogenase, mannitol-1-phosphate dehydrogenase, malate dehydrogenase, glucose dehydrogenase, D-galactose dehydrogenase, glucose-6-phosphate dehydrogenase, catalase and /-esterase) and genetic (Nei’s statistics) and cluster analysis (UPGMA algorithm). Results: A highly frequent polyclonal pattern was observed in this population of ORSA isolates, suggesting various sources of contamination or microbial dispersion. Genetic relationship analysis showed a high degree of polymorphism between the strains, and it revealed three taxa (A, B and C) distantly genetically related (0.653dij1.432) and fifteen clusters (I to XV) moderately related (0.282dij0.653). These clusters harbored two or more highly related strains (0dij0.282), and the existence of microevolutionary processes in the population of ORSA. Conclusion: This research reinforces the hypothesis of the existence of several sources of contamination and/or dispersal of ORSA of clinical and epidemiologically importance, which could be associated with carriers (patients) and dental environmental (air).

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Candida species biotypes and polyclonality of potentially virulent Candida albicans isolated from oral cavity of patients with orofacial clefts

et al. 2021

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Objectives This study evaluated the incidence of Candida species, and the genetic diversity and virulence of C. albicans of the oral cavity from patients with cleft lip and palate (CLP). Materials and methods Oral samples were investigated by microbiological and species-specific PCR methods. The genetic diversity of C. albicans was established using isoenzyme markers, Nei's statistics, and clustering analysis. Hydrolytic enzymes (SAPs and PLs) were analyzed in vitro. Results Oral colonization by Candida species was observed in 29 patients with CLP (65.9%), and C. albicans was highly prevalent. SAP and PL activities were observed in 100% and 51.9% of isolates, respectively. High genetic diversity and patterns of monoclonal and polyclonal oral colonization by C. albicans were observed among patients with CLP. Two major polymorphic taxa (A and B) and other minor polymorphic taxa (C to J) were identified. Only one of the 16 clusters (taxon A) harbored strains from patients with and without CLP, whereas other clusters harbored strains exclusively from CLP patients. Conclusions The anatomical conditions of the oral cavity of patients with CLP contribute to the high incidence of Candida species (C. albicans, C. krusei, C. tropicalis, and/or Candida spp.). Data suggest high genetic diversity of potentially virulent C. albicans strains in the oral cavity of CLP patients. Clinical relevance Microbiological niches in orofacial clefts can contribute to the emergence of a relative clinical genotypic identity of C. albicans. However, orofacial rehabilitation centers can contribute to the direct and indirect sources of transmission and propagation of Candida species.

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Disparity between Multilocus Enzyme Electrophoresis, Microsatellite Markers and Pulsed-Field Gel Electrophoresis in epidemiological tracking of Candida albicans

Cited by 8 publications

References 72 publications

Molecular methods for strain typing of Candida albicans : a review

Molecular methods for strain typing of Candida albicans : a review

Isoenzyme genotyping and phylogenetic analysis of oxacillin-resistance Staphylococcus aureus isolates

Candida species biotypes and polyclonality of potentially virulent Candida albicans isolated from oral cavity of patients with orofacial clefts

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