Disinfection of Impression Materials with Glutaraldehyde, Ultraviolet Radiation, and Autoclave: A Comparative Study

Abstract

Background: The present study was conducted to compare various methods of disinfection of impression materials such as glutaraldehyde, ultraviolet (UV) radiation, and autoclave. Materials and Methods: The present study was conducted on eighty alginate impression materials which were disinfectant with 2.2% glutaraldehyde, UV radiation, and autoclave. The pre- and postbacterial count was assessed. Results: The mean premicrobial contamination in Group I was 362.2 106 colony-forming unit (CFU)/ml, in Group II was 306.4 106 CFU/ml, and in Group III was 336.2 106 CFU/ml. The mean postmicrobial contamination in Group I was 65.2 106 CFU/ml, in Group II was 76.7 106 CFU/ml, and in Group III was 28.4 106 CFU/ml. Conclusion: The authors found that autoclave proved to be better in terms of reduction in bacterial colonies as compared to glutaraldehyde and UV radiation.

Evaluation of Antimicrobial Efficacy of UVC Radiation, Gaseous Ozone, and Liquid Chemicals Used for Disinfection of Silicone Dental Impression Materials

“…However, due to the fact that this study was conducted using impressions taken from the patients, there was no detailed information on the specific microbial strains present in the samples [ 16 ]. Another study demonstrated lower UV radiation effectiveness than autoclave, used for the elimination of pathogenic microorganisms from alginate impressions [ 17 ]. Godbole et al revealed a lack of a significant negative influence of UV light disinfection on the dimensional stability of polyvinyl siloxane impressions.…”

“…It must be taken into account that various disinfectants also pose a risk of alterations of dimensions and surface chemistry (e.g., wettability) of dental impressions. To avoid the negative influence of these chemical agents on different material properties of dental impressions, researchers are looking for other methods of disinfection, such as gaseous ozone [ 13 ], microwave irradiation [ 14 , 15 ], ultraviolet radiation [ 16 , 17 , 18 ], or electrolyzed oxidizing water [ 19 , 20 ]. Such alternative techniques may be particularly beneficial to oral health maintenance when applied to the most basic impression material used nowadays, based on sodium alginate.…”

“…For these reasons, a detailed investigation of the potential of novel techniques of disinfection, other than spraying and immersion, is still highly recommended. In particular, UVC radiation and ozone, despite few previous research reporting usefulness of these methods for dental impressions disinfection, are still not gold standard techniques [ 13 , 16 , 17 , 18 , 19 ]. Apart from their application for surface disinfection, both these methods have the potential as a support for the treatment of endodontic infections and inflammation in root canals [ 27 , 28 ].…”

Evaluation of the effect of chemical disinfection and ultraviolet disinfection on the dimensional stability of polyether impression material: an in-vitro study

“…Using UV light tubes with higher wattage has been found to significantly reduce colony counts in a shorter time. The most effective antibacterial efficiency through UV light exposure occurs at 24 watts (3750 µw/cm2), and higher wattages result in a faster reduction in C. albicans colony counts, ultimately reaching zero [8,14]. Samra et al (2018) proposed that the ultraviolet method is a favorable choice for disinfecting impressions without compromising their dimensional stability [15].…”

“…Therefore, dentists and dental staff must be familiar with standardized procedures for disinfecting dental impressions and casts. The literature describes various methods of impression disinfection, including chemical disinfection, microwave treatment, autoclaving, and ultraviolet radiation, each with its advantages, disadvantages, and effects on impression materials and casts [ 8 , 9 ]. Recently, impression materials have been directly integrated with antimicrobials and nanoparticles, enabling the material to possess self-disinfecting properties.…”