Discovery and Correction of Spurious Low Platelet Counts due to EDTA‐Dependent Pseudothrombocytopenia

Lin, Jianhua; Luo, Yun; Yao, Shaoyu; Yan, Meilin; Li, Jianpei; Ouyang, Wen-ting; Kuang, Miao-huan

doi:10.1002/jcla.21818

Cited by 30 publications

(39 citation statements)

References 39 publications

(54 reference statements)

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“…On the other hand, platelet clumps and giant platelets owing to their large size are not counted as platelets in automated analyzers. All these lead to inaccurate platelet counts in an automated hematology analyzer [8][9][10][11] . Hence platelet count values obtained from automated analyzers should be crosschecked by some other method particularly in cases of thrombocytopenia [12] .…”

Section: Discussionmentioning

confidence: 99%

A novel method to estimate platelet counts from peripheral smears: A study comparing a new method of platelet estimation with existing methods.

Sudalaimuthu¹,

Ganapathy²,

Rajendran³

et al. 2017

APALM

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Background: Automated hematology analyzers have a low degree of accuracy in predicting platelet counts in patients with thrombocytopenia. Hence automated platelet counts should be crosschecked by reviewing the peripheral blood smear in cases of thrombocytopenia. However existing methods for platelet count estimation from peripheral blood smears have certain drawbacks. Hence we suggest a new method to estimate platelet count from peripheral smear and compared it with the existing methods. Conclusion:Platelet count estimation based on platelet/RBC ratio in ten oil immersion fields and total RBC count is a simple and reliable method to estimate platelet counts from peripheral smears.

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Section: Discussionmentioning

confidence: 99%

A novel method to estimate platelet counts from peripheral smears: A study comparing a new method of platelet estimation with existing methods.

Sudalaimuthu¹,

Ganapathy²,

Rajendran³

et al. 2017

APALM

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“…However, the scattergram displayed from the immature myeloid information (IMI) channel in XE-2100 improved platelet aggregates detection. Therefore, screening for platelet clumps in XE-2100 by using platelet clump flag along with the images provided by the IMI channel is convenient [11]. Sensitivity and specificity for platelet clumps detection may vary according to the flag threshold selected by the user, which was the reason why our results highlighted the point that each laboratory should determine specific threshold flags according to their population.…”

mentioning

confidence: 69%

Performance evaluation of low platelet count and platelet clumps detection on Mindray BC-6800 hematology analyzer

Fuster

Andino

Laíz

2016

Clinical Chemistry and Laboratory Medicine (CCLM)

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“…Some of these anticoagulants need to be prepared extemporaneously. Another possibility is the addition of calcium chloride [44], anti-platelet drugs (acetylsalicylic acid, prostaglandin E 1 , apyrase, monoclonal antibodies directed against GpIIb/IIIa), potassium azide, kanamycin, amikacin, or other aminosides [54][55][56], which prevent the formation of clumps or dissociate them.…”

Section: Alternatives To Edtamentioning

confidence: 99%

Platelet Counting: Ugly Traps and Good Advice. Proposals from the French-Speaking Cellular Hematology Group (GFHC)

Baccini

Geneviève

Jacqmin

et al. 2020

JCM

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Despite the ongoing development of automated hematology analyzers to optimize complete blood count results, platelet count still suffers from pre-analytical or analytical pitfalls, including EDTA-induced pseudothrombocytopenia. Although most of these interferences are widely known, laboratory practices remain highly heterogeneous. In order to harmonize and standardize cellular hematology practices, the French-speaking Cellular Hematology Group (GFHC) wants to focus on interferences that could affect the platelet count and to detail the verification steps with minimal recommendations, taking into account the different technologies employed nowadays. The conclusions of the GFHC presented here met with a "strong professional agreement" and are explained with their rationale to define the course of actions, in case thrombocytopenia or thrombocytosis is detected. They are proposed as minimum recommendations to be used by each specialist in laboratory medicine who remains free to use more restrictive guidelines based on the patient's condition.

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Discovery and Correction of Spurious Low Platelet Counts due to EDTA‐Dependent Pseudothrombocytopenia

Abstract: The method of screening for platelet clumping with the help of XE5000 images is convenient. The decline in the platelet count is related to the length of time and the intensity of chelation. Amikacin supplement is not always effective for correcting platelet counts in vitro.

Cited by 30 publications

References 39 publications

A novel method to estimate platelet counts from peripheral smears: A study comparing a new method of platelet estimation with existing methods.

A novel method to estimate platelet counts from peripheral smears: A study comparing a new method of platelet estimation with existing methods.

Performance evaluation of low platelet count and platelet clumps detection on Mindray BC-6800 hematology analyzer

Platelet Counting: Ugly Traps and Good Advice. Proposals from the French-Speaking Cellular Hematology Group (GFHC)

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