Directing stem cell fate on hydrogel substrates by controlling cell geometry, matrix mechanics and adhesion ligand composition

Lee, Junmin; Abdeen, Amr A.; Zhang, Douglas; Kilian, Kristopher A.

doi:10.1016/j.biomaterials.2013.07.074

Cited by 248 publications

(250 citation statements)

References 32 publications

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“…b Electrical References: (Jaffe and Stern, 1979;Patel and Poo, 1982;Hotary and Robinson, 1991;Davenport and McCaig, 1993;Metcalf and Borgens, 1994;Yao et al, 2008Yao et al, , 2009Yao et al, , 2011Graves et al, 2011;Koppes et al, 2014;Kim et al, 2016;Ma et al, 2016). c Mechanical Stiffness References: (Balgude et al, 2001;Discher et al, 2005;Jiang et al, 2010;Keung et al, 2012;Lee et al, 2013;Zhang et al, 2014;Mosley et al, 2017).…”

Section: Topographical Cues Drive Alignment and Directionalitymentioning

confidence: 99%

“…The importance of substrate mechanics as a cue is evident during differentiation of stem cells in environments of controlled stiffness. Increasingly higher stiffness encourages their differentiation into muscle [elastic modulus (E) ∼10 kPa] or bone (E > 30 kPa), whereas a lower stiffness on the order of hundreds of Pa encourages differentiation into neurons (Lee et al, 2013). This is consistent with the elastic modulus within the central and peripheral nervous systems, which ranges between 0.5 and 1 kPa, and the shear stiffness of human brain tissue in vivo, which has been measured between 2 and 3 kPa (Lee et al, 2013;Bai et al, 2014;Hiscox et al, 2016).…”

Section: Manipulation Of Substrate Stiffnessmentioning

confidence: 99%

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Biomaterials for Enhancing Neuronal Repair

Cangellaris

Gillette

2018

Front. Mater.

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As they differentiate from neuroblasts, nascent neurons become highly polarized and elongate. Neurons extend and elaborate fine and fragile cellular extensions that form circuits enabling long-distance communication and signal integration within the body. While other organ systems are developing, projections of differentiating neurons find paths to distant targets. Subsequent post-developmental neuronal damage is catastrophic because the cues for reinnervation are no longer active. Advances in biomaterials are enabling fabrication of micro-environments that encourage neuronal regrowth and restoration of function by recreating these developmental cues. This mini-review considers new materials that employ topographical, chemical, electrical, and/or mechanical cues for use in neuronal repair. Manipulating and integrating these elements in different combinations will generate new technologies to enhance neural repair.

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Section: Topographical Cues Drive Alignment and Directionalitymentioning

confidence: 99%

Section: Manipulation Of Substrate Stiffnessmentioning

confidence: 99%

Biomaterials for Enhancing Neuronal Repair

Cangellaris

Gillette

2018

Front. Mater.

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“…27 Therefore, the production of adequate scaffolds for the proliferation and anchorage of MSCs is fundamental to engineer functional cardiac tissue constructs in vitro. 28,29 Some studies have gone further and focused on the development of CNTs in nanofiber scaffolds that simulate the architecture of the myocardium tissue repairing the affected cardiac areas. 30,31 A significant advantage derived from working with primary cultures of MSCs is to ensure a clean and clear genetic background without the presence of genetic disorders such as those accumulated in traditional cell lines previously preestablished and characterized.…”

Section: Introductionmentioning

confidence: 99%

“…36 Therefore, MSCs are an excellent model to evaluate several biological parameters such as proliferation, apoptosis, anchorage, and cytotoxicity. 29 Although the safety of MSCs application has been emphasized in several reports, 37 their exposure to different carcinogens in functionalization process could initiate some types of cancer, but until now, the role of fCNTs as possible carcinogen in MSCs remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Evaluating the biological risk of functionalized multiwalled carbon nanotubes and functionalized oxygen-doped multiwalled carbon nanotubes as possible toxic, carcinogenic, and embryotoxic agents

Lara-Martínez

Massó

Palacios

et al. 2017

IJN

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Carbon nanotubes (CNTs) have been a focus of attention due to their possible applications in medicine, by serving as scaffolds for cell growth and proliferation and improving mesenchymal cell transplantation and engraftment. The emphasis on the benefits of CNTs has been offset by the ample debate on the safety of nanotechnologies. In this study, we determine whether functionalized multiwalled CNTs (fMWCNTs) and functionalized oxygen-doped multiwalled CNTs (fCOxs) have toxic effects on rat mesenchymal stem cells (MSCs) in vitro by analyzing morphology and cell proliferation and, using in vivo models, whether they are able to transform MSCs in cancer cells or induce embryotoxicity. Our results demonstrate that there are statistically significant differences in cell proliferation and the cell cycle of MSCs in culture. We identified dramatic changes in cells that were treated with fMWCNTs. Our evaluation of the transformation to cancer cells and cytotoxicity process showed little effect. However, we found a severe embryotoxicity in chicken embryos that were treated with fMWCNTs, while fCOxs seem to exert cardioembryotoxicity and a discrete teratogenicity. Furthermore, it seems that the time of contact plays an important role during cell transformation and embryotoxicity. A single contact with fMWCNTs is not sufficient to transform cells in a short time; an exposure of fMWCNTs for 2 weeks led to cell transformation risk and cardioembryotoxicity effects.

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“…The simplicity of this approach suggests new avenues to pursue in decoding relationships between insoluble and soluble signals for controlling stem cell fate. Some groups have made microarrayed libraries of polymeric materials for high-throughput characterization of stem cell behavior (9, 10); other groups have focused on arrays that mimic cell-cell and cellmatrix interactions (11,12) and include control of matrix elasticity (13). Although the various approaches can all potentially meet the economies of scale, combining the approach of Wrighton et al with approaches at mimicry may prove especially helpful in elucidating the biological mechanisms that promote and maintain specific tissue lineages.…”

mentioning

confidence: 99%