Directed regulation of the human microbiome: contribution to the solution of the demographic problem in Russia

Abstract

In order to solve the demographic problem that has arisen in Russia, comprehensive multilateral approaches are required, aimed at increasing the birth rate and reducing child and adult mortality. One approach to solve this problem is directed regulation of the human microbiome.

S-layer protein 2 of vaginal Lactobacillus crispatus 2029 enhances growth, differentiation, VEGF production and barrier functions in intestinal epithelial cell line Caco-2

“…aeruginosa 27853) to Caco-2 cells was inhibited in a dose-dependent manner by the addition of Slp2 in the medium. At the same time, Caco-2 cultivated in the presence of Slp2 increased the adhesion of Bifidobacterium longum subspecies infantis BLI 2780 to the enterocytes of these line[24].…”

“…We found that Slp2 increases the activity of intestinal ALP (Figure 3A) in Caco-2 enterocytes and inhibits the adhesion of NEC-inducing pathogens to these intestinal cells (Table 2). At the same time, Slp2 stimulates the adhesion of bifidobacteria on Caco-2 enterocytes, ensuring the formation of normal parietal microflora of the newborns [24]. Therefore, this could be a mechanism behind the bifidobacteria correlation with Slp2 and ALP activities.…”

“…Slp2 was extracted and purified according to [45] with modifications [24]. Briefly, Slp2 was extracted from LC2029 strain by 5M LiCl.…”

“…High expression of only the slp2 gene was detected. Slp2 forms regular two-dimensional structure around the LC2029 cells and is also secreted into the medium [24].…”

“…Our previous data was demonstrated that soluble Slp2 inhibits intestinal pathogen-induced activation of caspase-9 and caspase-3 as apoptotic biomarkers in the human intestinal Caco-2 cells [24]. The effects of Slp2 on the human small intestine remain poorly understood.…”