Directed evolution and expression tuning of geraniol synthase for efficient geraniol production in <i>Escherichia coli</i>

Recent Advances and Multiple Strategies of Monoterpenoid Overproduction in Saccharomyces cerevisiae and Yarrowia lipolytica

“…49 Directed evolution is an efficient enzymatic modification technique to improve the catalytic efficiency of key enzymes. 50 Some enzymes in which the relationship between structure and function is unclear, so it is not feasible to perform rational design and modification. Directed evolution has successfully produced superior mutants of various enzymes, enhancing the production capabilities of many natural products.…”

“…Directed evolution is an efficient enzymatic modification technique to improve the catalytic efficiency of key enzymes . Some enzymes in which the relationship between structure and function is unclear, so it is not feasible to perform rational design and modification.…”

The construction and optimization of engineered yeast chassis for efficient biosynthesis of 8‐hydroxygeraniol

“…Accumulation of sufficient geraniol is also critical for improving the yield of 8‐hydroxygerainol, which often requires metabolic engineering of the biosynthesis pathway. Several strategies have been shown to increase the supply of geraniol, including truncation of GES enzyme or utilization of fusion tag 12 to improve its heterologous expression 13 , 14 , engineering of mevalonate (MVA) pathway to enhance GPP concentration 15 , 16 , and directed evolution of GES to improve its catalytic activity 17 , 18 . Despites these successes, little efforts focused on systematic optimization of the metabolic network to minimize the incompatibility between the heterologous biosynthesis pathway and the chassis.…”

Engenharia metabólica da levedura Yarrowia lipolytica para a produção heteróloga do monoterpeno geraniol.

“…Nevertheless, scientific publications describing heterologous geraniol production employ mostly E. coli and S. cerevisiae (CHACÓN et al, 2019;GERKE et al, 2020;JIANG et al, 2017;LIU et al, 2016;TASHIRO et al, 2017;WANG et al, 2021;YEE et al, 2019;ZHAO et al, 2017;ZHOU et al, 2014). This is due to the accumulated knowledge and the plethora of molecular biology and genome editing tools accessible for these model organisms.…”