Directed assembly of cell-laden hydrogels for engineering functional tissues

Kachouie, Nezamoddin N.; Du, Yumin; Bae, Hojae; Khabiry, Masoud; Ahari, Amirhossein F.; Zamanian, Behnam; Fukuda, Junji; Khademhosseini, Ali

doi:10.4161/org.6.4.12650

Cited by 65 publications

(55 citation statements)

References 68 publications

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“…Cellular approach employs small units of living cells for the construction of 3D tissues. This approach includes cell sheet stacking [1,2], cell sheet sandwiching [3], cell sheet wrapping [4,5], and 3D cell accumulation [6,7]. Tube-like and 3D thick tissues are mostly fabricated by such methods.…”

Section: Introductionmentioning

confidence: 99%

Flexible microscaffold facilitating the in vitro construction of different cellular constructs

et al. 2014

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This paper presents a flexible microscaffold to facilitate the fabrication of different cellular constructs which could be used as the building units for the construction of a larger tissue with a complex structure. The device consists of a 6 × 6 array of membrane actuators, made of Polydimethylsiloxane. The superiority of membrane actuators helps preventing the leakage of culture medium and allows for the formation of various temporary scaffolds. In biological test, NIH3T3 cells were seeded on the scaffold provided. The positive pressure applied to membrane actuators enables the formation of the scaffold for construction of hole array-patterned and round flat cell sheets while the negative pressure applied enables the scaffold for construction of spherical cellular aggregates. The results after 2-day cultivation show that the micropatterned cell sheet has a thickness of about 100 μm and a hole diameter of about 200 μm. In addition, the round flat cell sheets have a diameter of about 623.87 μm, and the spherical aggregates have a diameter of about 280 μm. These suggest the possibility of using our device to prepare many different cellular constructs with more complex structures in future biological applications.

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Section: Introductionmentioning

confidence: 99%

Flexible microscaffold facilitating the in vitro construction of different cellular constructs

et al. 2014

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“…Bottom-up approaches include cell-encapsulation with microscale hydrogels, cell aggregation by self-assembly, generation of cell sheets, and direct printing of cells [58]. These complex tissue blocks can be assembled using various methods including microfluidics [59], magnetic fields [60], acoustic fields [61], and surface tension [62]. These methods are relatively easy and have provided a solid foundation for the fabrication of scaffolds.…”

Section: Manufacturing Of Scaffolds With 3-d Printing Technologymentioning

confidence: 99%

Application of 3-D Printing for Tissue Regeneration in Oral and Maxillofacial Surgery: What is Upcoming?

Keyhan¹,

Fallahi²,

Jahangirnia³

et al. 2018

Biomaterials in Regenerative Medicine

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The ultimate goal of any surgical procedure is to improve perioperative form and function and to minimize operative and postoperative morbidity. In recent years, many exciting and novel technological advances have been introduced in the field of oral and maxillofacial surgery. One example of such technology that is continuing to increase in prevalence is the use of 3-dimensional (3-D) printing techniques with special properties, which seems hopeful for practitioners in the field of regenerative medicine. Tissue engineering is a critical and important area in biomedical engineering for creating biological alternatives for grafts, implants, and prostheses. One of the main triad bases for tissue engineering is scaffolds, which play a great role for determining growth directions of stem cells in a 3-dimensional aspect. Mechanical strength of these scaffolds is critical as well as interconnected channels and controlled porosity or pores distribution. However, existing 3-D scaffolds proved less than ideal for actual clinical applications. In this chapter, we review the application and advancement of rapid prototyping (RP) techniques in the design and creation of synthetic scaffolds for use in tissue engineering. Also, we survey through new and novel merging era of "bioprinting."

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“…Due to the strict requirements, biocompatible self-assembly methods strongly rely on hydrogel technologies [5,6,58,63,[90][91][92][93]. Utilization of hydrogel additionally enables encapsulation of cells at various concentrations and combination of different cell types [58,[90][91][92][93].…”

Section: Self-assembly Of Cell-laden Hydrogel Building Blocksmentioning

confidence: 99%

“…The fabrication of tissue-like structures and 3D cellular constructs requires mimicking structural hierarchy, heterogeneity and variety of tissues and organs [60][61][62][63]. One of the most critical aspects to engineering cellular constructs is multi-cell co-culture with 3D spatial control [60].…”

Section: Assembly Of Cellular Constructsmentioning

confidence: 99%

“…Among various strategies of self-assembly methods based on molecular interactions [6,63,[66][67][68][69][70][71][73][74][75][76][77][78][79][80][81][82][83][84][85][86][87][88][89][90], only few strategies can be utilized for tissue engineering; aqueous condition, salt and pH concentration as well as cell culture condition in temperature and gas concentration need to be maintained, external fields and forces cannot interfere with life functions of cells. Due to the strict requirements, biocompatible self-assembly methods strongly rely on hydrogel technologies [5,6,58,63,[90][91][92][93].…”

Section: Self-assembly Of Cell-laden Hydrogel Building Blocksmentioning

confidence: 99%

See 1 more Smart Citation

Fabrication of 3D Cellular Tissue Utilizing MEMS Technologies

Yoshida

Serien

Tomoike

et al. 2015

Hyper Bio Assembler for 3D Cellular Systems

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This chapter focuses on the use of micro-sized cell culture substrates fabricated by micro electro mechanical systems (MEMS) technologies in the field of three-dimensional (3D) cellular tissue constructions. First, we provide a brief overview of MEMS-based tissue engineering methods, and explain why the micro-sized cell-laden substrates are important. Second, we explain the fabrication processes of the micro-sized cell-laden substrates. The fabrication of micro-sized substrates is described by focusing on their materials, structures, cell culture processes, and handling. Third, their applications for single cell handling is described by providing information on observation, collection, and arrangement. Finally, assembly of 3D cellular constructs is explained by pick-and-place assembly, self-assembly, and self-folding methods. Key advantages of the micro-sized substrates are the ability of manipulating various types of adherent cells and also spatial control of cells in 3D cellular tissues. The single cell handling ability facilitates the cell-cell interaction analysis in the field of pharmacology, and 3D cellular assembly ability will open the route for fabrication of spatially-controlled heterogeneous 3D cellular tissues in the regenerative medicine field.

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Directed assembly of cell-laden hydrogels for engineering functional tissues

Cited by 65 publications

References 68 publications

Flexible microscaffold facilitating the in vitro construction of different cellular constructs

Flexible microscaffold facilitating the in vitro construction of different cellular constructs

Application of 3-D Printing for Tissue Regeneration in Oral and Maxillofacial Surgery: What is Upcoming?

Fabrication of 3D Cellular Tissue Utilizing MEMS Technologies

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