Direct somatic embryogenesis with high frequency plantlet regeneration and successive cormlet production in saffron (Crocus sativus L.)

Devi, Kiran; Sharma, Madhu; Ahuja, Paramvir Singh

doi:10.1016/j.sajb.2014.04.006

Cited by 30 publications

(23 citation statements)

References 40 publications

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“…Direct formation of embryos was observed on MS medium supplemented with 2.5 μM TDZ and 2.0 μM picloram (Devi et al 2014). Direct formation of embryos was observed on MS medium supplemented with 2.5 μM TDZ and 2.0 μM picloram (Devi et al 2014).…”

Section: Crocus Sativus (Iridaceae)mentioning

confidence: 89%

Somatic Embryogenesis: Fundamental Aspects and Applications

Ochoa-Alejo¹,

Loyola‐Vargas²

2016

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Section: Crocus Sativus (Iridaceae)mentioning

confidence: 89%

Somatic Embryogenesis: Fundamental Aspects and Applications

Ochoa-Alejo¹,

Loyola‐Vargas²

2016

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“…At the same time picloram can be used in plant tissue culture tests for somatic embryogenesis in saffron (Devi et al, 2014) and in Agapanthus praecox ssp. minimus (Yaacob et al, 2012) for shoot proliferation in Lilium michiganense (Ault and Siqueira, 2008).…”

Section: Resultsmentioning

confidence: 99%

“…On the other hand there are a few studies (Aytekin and Acıkgoz, 2008) related with usage of plant growth regulators for saffron corm production under the field condition. Mostly the studies (Plessner et al, 1990, Sharma et al 2008, Zeybek et al, 2012, Devi et al, 2011, 2014, Cavusoglu et al, 2013 on the effects of plant growth regulators have been done in in vitro.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Exogenously Applied Plant Growth Regulators on Plant Development of Saffron (Crocus sativus L.)

Çavuşoğlu¹

2017

jist

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Plant growth regulators have important roles in plant growth and development. The externally applicable chemicals belong to different action classes and each one has a crucial and effective role at different plant growing stages. Saffron (Crocus sativus L.) is one of the most important and valuable medicinal and aromatic plant, belongs to Iridaceae family. Because of impossibility of generative propagation in nature, growing can be maintened asexually with daughter corms occured each year. In this experiment, the effects of exogenously applied paclobutrazol, indole-3-butyric acid, zeatin and picloram on saffron developmental stages were studied. Data were focused on aerial part and corm related characters which is important in developmental findings in this plant. In the treatments, paclobutrazol (10 or 20 mg L -1 ), indole-3-butyric acid (1 or 3 mg L -1 ), zeatin (1 or 3 mg L -1 ) and picloram (5 or 10 mg L -1 ) were applied alone besides control to saffron 45 days after planting to the soil under greenhouse condition. According to the data picloram concentrations have a retardant role in nodium activation, leaf number and corm developmental characters (number, weight, diameter and yield of daughter corm) but cause the highest plant height (60.98 cm in 10 mg L -1 and 57.37 cm in 5 mg L -1 ). In 10 mg L -1 paclobutrazol treatments resulted as the best corm production (469 kg da -1 ) than the other treatments and control. Zeatin at 3 mg L -1 concentration was found effective on the best nodium activation (2.81 active nodes/corm). In conclusion, saffron development is affected significantly by externally applied plant growth regulators under soil condition and the application methods can be progressed in studies on aimed parts of the plant. Keywords:Crocus sativus, indole-3-butyric acid, paclobutrazol, picloram, zeatin ÖZET: Dışsal uygulanabilen kimyasallar olan ve farklı etki sınıfları içinde bulunan bitki gelişim düzenleyicilerinin her biri bitki büyüme ve gelişiminin farklı aşamaları üzerinde hayati ve önemli işlevlere sahiptir. Tıbbi ve aromatik bitkiler içinde en önemli ve değerli bitkilerden biri olan safran (Crocus sativus L.) Iridaceae familyasına ait olup, doğal olarak generatif yolla üretilmesi mümkün olmadığından bitki gelişimi aseksüel olarak her yıl meydana gelen yavru kormlardan sağlanmaktadır. Bu araştırmada dışsal olarak uygulanan paclobutrazol, indole-3-butyric acid, zeatin and picloram maddelerinin safran gelişimi üzerine etkileri çalışılmıştır. Bitki gelişimi için önemli parametreler olan korm ve toprak üstü aksamı ile ilişkili karakterlerle ilgili veriler toplanmıştır. Çalışmada kontrol parsellerinin yanında paclobutrazol, indole-3-butyric acid, zeatin ve picloram bitki dikiminden 45 gün sonra cam serada toprağa uygulanmıştır. Elde edilen verilere göre picloram konsantrasyonları nodyum aktivasyonu, yaprak sayısı ve korm gelişim özellikleri (korm sayısı, ağırlığı, çapı ve yavru korm verimi) için geciktirici bir rol oynarken, en yüksek bitki boyu (10 mg L -1 de 60.98 cm ve 5 mg L -1 de ...

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“…Shoots, formed from these embryos, were propagated in BAP and NAA MS media (27 and 1 μM, respectively). Interestingly, in vitro formed shoots produced cormlets similar in size and behavior to those formed by field plants (Devi et al 2014).…”

Section: Crocus Sativus (Iridaceae)mentioning

confidence: 85%

“…Direct formation of embryos was observed on MS medium supplemented with 2.5 μM TDZ and 2.0 μM picloram (Devi et al 2014). Secondary embryo formation occurred on the same medium, but only after reducing picloram to 1.0 μM.…”

Section: Crocus Sativus (Iridaceae)mentioning

confidence: 87%

Application of Somatic Embryogenesis to Secondary Metabolite-Producing Plants

Vázquez‐Flota

Monforte‐González

Miranda-Ham

2016

Somatic Embryogenesis: Fundamental Aspects and Applications

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Plants display an amazing biosynthetic capacity. To date, around 200,000 different chemical compounds have been isolated from them. Only a relatively few of these compounds are common to all plant species, since they are involved in basic or primary cell processes, such as energy metabolism. However, the broader plant chemical diversity corresponds to those compounds showing a restricted distribution among only a few taxonomically related species and which are not involved in primary metabolic pathways. These compounds are called secondary or specialized metabolites and they have important roles in numerous plant-environment interactions. Aside from these functions, secondary metabolites, and the plants bearing them, represent highly regarded commercial products given their pharmaceutical, flavoring, aromatic, coloring, and poisonous properties. In here, we present some selected examples of secondary metabolite-producing plants for which efficient protocols of somatic embryogenesis have been developed. The review covers mainly plants producing fine chemicals, used either in pharmaceutical or food industries. As shown, the development of somatic embryogenesis procedures could respond to two main goals: the genetic transformation of a given plant species, or the massive propagation of selected materials. Furthermore, the use of such protocols for the generation of diversity through indirect embryo formation is also presented.

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Direct somatic embryogenesis with high frequency plantlet regeneration and successive cormlet production in saffron (Crocus sativus L.)

Cited by 30 publications

References 40 publications

Somatic Embryogenesis: Fundamental Aspects and Applications

Somatic Embryogenesis: Fundamental Aspects and Applications

The Effect of Exogenously Applied Plant Growth Regulators on Plant Development of Saffron (Crocus sativus L.)

Application of Somatic Embryogenesis to Secondary Metabolite-Producing Plants

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