Direct plasma injection for high-performance liquid chromatographic–mass spectrometric quantitation of the anxiolytic agent CP-93 393

Needham, Shane; Cole, Mark J.; Fouda, Hassan G.

doi:10.1016/s0378-4347(98)00353-3

Cited by 38 publications

(32 citation statements)

References 15 publications

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“…Consequently, there is considerable interest in developing an on-line sample preparation procedure to overcome the need for the time-consuming evaporation and reconstitution steps that are used in typical off-line sample preparation methods. On-line direct analyses of biological fluids have been reported, using a column switching technique with a restricted access colum 31 or solid-phase extraction cartridge. 32 Thus, the focus of the present work was to compare APCI LC/MS with APPI LC/MS in terms of detection sensitivity and matrix effects, to optimize the experimental factors that affect the performance of the competing API techniques, and to develop a column switching technique as a on-line sample preparation method for patulin in apple juice.…”

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confidence: 99%

Liquid chromatography/mass spectrometric determination of patulin in apple juice using atmospheric pressure photoionization

Takino

Daishima

Nakahara

2003

Rapid Comm Mass Spectrometry

104

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This paper describes a comparison between atmospheric pressure chemical ionization (APCI) and the recently introduced atmospheric pressure photoionization (APPI) technique for the liquid chromatography/mass spectrometric (LC/MS) determination of patulin in clear apple juice. A column switching technique for on-line extraction of clear apple juice was developed. The parameters investigated for the optimization of APPI were the ion source parameters fragmentor voltage, capillary voltage, and vaporizer temperature, and also mobile phase composition and flow rate. Furthermore, chemical noise and signal suppression of analyte signals due to sample matrix interference were investigated for both APCI and APPI. The results indicated that APPI provides lower chemical noise and signal suppression in comparison with APCI. The linear range for patulin in apple juice (correlation coefficient >0.999) was 0.2-100 ng mL(-1). Mean recoveries of patulin in three apple juices ranged from 94.5 to 103.2%, and the limit of detection (S/N = 3), repeatability and reproducibility were 1.03-1.50 ng mL(-1), 3.9-5.1% and 7.3-8.2%, respectively. The total analysis time was 10.0 min.

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confidence: 99%

Liquid chromatography/mass spectrometric determination of patulin in apple juice using atmospheric pressure photoionization

Takino

Daishima

Nakahara

2003

Rapid Comm Mass Spectrometry

104

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“…However, such approaches did not work adequately for the recovery of the penicillins from the matrix containing Micafungin (poor recovery rate up to 40%, data not shown), and careful attention was required to handle a trace amount of the penicillins during the extraction procedure. To improve the accuracy of the method without extraordinary handling, an on-line extraction was examined using the column switching technique, [11][12][13][14] which enables reduction of the loss of analytes during the extraction procedure. Figure 3 shows diagrams of an HPLC equipped with a valve for the column switching technique.…”

Section: Resultsmentioning

confidence: 99%

Quantitative Determination Method for Trace Amount of Penicillin Contaminants in Commercially Available Drug Product by HPLC Coupled with Tandem Mass Spectrometry

Takada

Adachi

Kihara

et al. 2005

CHEMICAL & PHARMACEUTICAL BULLETIN

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“…As little as 0.5 ng/mL of these compounds could be quantified by this method. An anxiolytic under clinical evaluation (CP-93 393) was also analyzed by online extraction from plasma by a RAM column (Needham et al, 1998) packed with porous silica coated with a1-acid glycoprotein with C 18 internal surface (Bio Trap 500 column). Recently, Fitzgerald et al (1999) developed a broad-spectrum LC/LC/LC-MS/MS to determine 17 basic drugs from different class (opiates, amphetamines, benzodiazepines, etc.)…”

Section: Hplc-msmentioning

confidence: 99%

Hyphenated chromatographic methods for biomaterials

Al-Dirbashi¹,

Nakashima²

2000

Biomed. Chromatogr.

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The improvement in hyphenated analytical techniques has significantly widened their applications to the analysis of biomaterials. In this article, we discuss recent advances in applications of hyphenated chromatographic techniques including capillary electrophoresis to the analyses of biological samples. As tools of separation, gas chromatography, high-performance liquid chromatography and capillary electrophoresis are considered with special emphasis on applications utilizing the hyphenation of these methods to mass spectrometry. Moreover, applications using other detection methods such as Fourier transform infrared spectroscopy hyphenated to gas chromatography and photodiode array detector combined with high-performance liquid chromatography or capillary electrophoresis are also discussed. Owing to their high sensitivity, luminescence-based detection systems such as laser-induced fluorescence and chemiluminescence are also included in this review.

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Direct plasma injection for high-performance liquid chromatographic–mass spectrometric quantitation of the anxiolytic agent CP-93 393

Cited by 38 publications

References 15 publications

Liquid chromatography/mass spectrometric determination of patulin in apple juice using atmospheric pressure photoionization

Liquid chromatography/mass spectrometric determination of patulin in apple juice using atmospheric pressure photoionization

Quantitative Determination Method for Trace Amount of Penicillin Contaminants in Commercially Available Drug Product by HPLC Coupled with Tandem Mass Spectrometry

Hyphenated chromatographic methods for biomaterials

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