Direct interaction of resistance gene and avirulence gene products confers rice blast resistance

Jia, Yulin; McAdams, Sean A.; Bryan, Gregory T.; Hershey, Howard P.; Valent, Barbara

doi:10.1093/emboj/19.15.4004

Cited by 990 publications

(696 citation statements)

References 57 publications

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“…In accordance with previous work (Mindrinos et al, 1994;Gopalan et al, 1996;Gilardi et al, 1998;Leister et al, 1996;Jia et al, 2000), the co-expression of the elicitor in cells from non-compatible hosts repeatedly resulted in a failure of or decrease in GUS activity when the GUS reporter gene was expressed under the control of the CaMV 35S promoter. The decrease in GUS activity has been associated with activation of cell death associated with the HR induction.…”

Section: Discussionsupporting

confidence: 90%

The coat protein of tobamovirus acts as elicitor of both L 2 and L 4 gene-mediated resistance in Capsicum

Gilardi¹,

García-Luque²,

Serra³

2004

Journal of General Virology

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In Capsicum, the resistance conferred by the L 2 gene is effective against all of the pepper-infecting tobamoviruses except Pepper mild mottle virus (PMMoV), whereas that conferred by the L 4 gene is effective against them all. These resistances are expressed by a hypersensitive response, manifested through the formation of necrotic local lesions (NLLs) at the primary site of infection. The Capsicum L 2 gene confers resistance to Paprika mild mottle virus (PaMMV), while the L 4 gene is effective against both PaMMV and PMMoV. The PaMMV and PMMoV coat proteins (CPs) were expressed in Capsicum frutescens (L 2 L 2 ) and Capsicum chacoense (L 4 L 4 ) plants using the heterologous Potato virus X (PVX)-based expression system. In C. frutescens (L 2 L 2 ) plants, the chimeric PVX virus containing the PaMMV CP was localized in the inoculated leaves and produced NLLs, whereas the chimeric PVX containing the PMMoV CP infected the plants systemically. Thus, the data indicated that the PaMMV CP is the only tobamovirus factor required for the induction of the host response mediated by the Capsicum L 2 resistance gene. In C. chacoense (L 4 L 4 ) plants, both chimeric viruses were localized to the inoculated leaves and produced NLLs, indicating that either PaMMV or PMMoV CPs are required to elicit the L 4 gene-mediated host response. In addition, transient expression of PaMMV CP into C. frutescens (L 2 L 2 ) leaves and PMMoV CP into C. chacoense (L 4 L 4 ) leaves by biolistic co-bombardment with a b-glucuronidase reporter gene led to the induction of cell death and the expression of host defence genes in both hosts. Thus, the tobamovirus CP is the elicitor of the Capsicum L 2 and L 4 gene-mediated hypersensitive response.

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Section: Discussionsupporting

confidence: 90%

The coat protein of tobamovirus acts as elicitor of both L 2 and L 4 gene-mediated resistance in Capsicum

Gilardi¹,

García-Luque²,

Serra³

2004

Journal of General Virology

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“…The first involves the Pi-ta protein from rice (Oryza sativa) and the AVR-Pita protein from the fungus Magnaportha grisea (Jia et al, 2000). Both yeast two-hybrid assays and an in vitro filter-binding assay demonstrated a physical interaction between the C-terminal Leu rich domain of Pi-ta and AVR-Pita.…”

Section: An Alternative Model For How R Proteins Mediate Pathogen Recmentioning

confidence: 99%

Guarding the Goods. New Insights into the Central Alarm System of Plants

Innes

2004

Plant Physiology

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“…Nine blast R genes were cloned and characterized [7]. The recognition of M. oryzae-secreted effector protein Avr-Pita by the R protein Pita inside the rice cell could activate innate immune response [8]. Two R genes, Pi9 and Pi21, confer broad-spectrum resistance against multiple races of M. oryzae [9,10].…”

Section: Introductionmentioning

confidence: 99%

Rice RING protein OsBBI1 with E3 ligase activity confers broad-spectrum resistance against Magnaporthe oryzae by modifying the cell wall defence

Zhong

et al. 2011

Cell Res

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Emerging evidence suggests that E3 ligases play critical roles in diverse biological processes, including innate immune responses in plants. However, the mechanism of the E3 ligase involvement in plant innate immunity is unclear. We report that a rice gene, OsBBI1, encoding a RING finger protein with E3 ligase activity, mediates broad-spectrum disease resistance. The expression of OsBBI1 was induced by rice blast fungus Magnaporthe oryzae, as well as chemical inducers, benzothiadiazole and salicylic acid. Biochemical analysis revealed that OsBBI1 protein possesses E3 ubiquitin ligase activity in vitro. Genetic analysis revealed that the loss of OsBBI1 function in a Tos17-insertion line increased susceptibility, while the overexpression of OsBBI1 in transgenic plants conferred enhanced resistance to multiple races of M. oryzae. This indicates that OsBBI1 modulates broad-spectrum resistance against the blast fungus. The OsBBI1-overexpressing plants showed higher levels of H 2 O 2 accumulation in cells and higher levels of phenolic compounds and cross-linking of proteins in cell walls at infection sites by M. oryzae compared with wild-type (WT) plants. The cell walls were thicker in the OsBBI1-overexpressing plants and thinner in the mutant plants than in the WT plants. Our results suggest that OsBBI1 modulates broad-spectrum resistance to blast fungus by modifying cell wall defence responses. The functional characterization of OsBBI1 provides insight into the E3 ligase-mediated innate immunity, and a practical tool for constructing broad-spectrum resistance against the most destructive disease in rice.

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Direct interaction of resistance gene and avirulence gene products confers rice blast resistance

Cited by 990 publications

References 57 publications

The coat protein of tobamovirus acts as elicitor of both L 2 and L 4 gene-mediated resistance in Capsicum

The coat protein of tobamovirus acts as elicitor of both L 2 and L 4 gene-mediated resistance in Capsicum

Guarding the Goods. New Insights into the Central Alarm System of Plants

Rice RING protein OsBBI1 with E3 ligase activity confers broad-spectrum resistance against Magnaporthe oryzae by modifying the cell wall defence

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