Direct injection of plasma to determine pseudoephedrine by high performance liquid chromatography with column switching

Simultaneous quantification of fexofenadine and pseudoephedrine in human plasma by liquid chromatography/tandem mass spectrometry with electrospray ionization: method development, validation and application to a clinical study

“…Various analytical procedures have been reported for separate quantitative determination of fexofenadine or pseudoephedrine in plasma. These include high‐performance liquid chromatography (HPLC) with ultraviolet (UV) detection for pseudoephedrine,6–10 HPLC with fluorescence detection for fexofenadine,11–14 gas chromatography (GC) for pseudoephedrine,15–17 LC/mass spectrometry (MS) for fexofenadine18 and pseudoephedrine,19 and LC/tandem mass spectrometry (MS/MS) for fexofenadine 20, 21…”

Sensitive bioassay for the simultaneous determination of pseudoephedrine and cetirizine in human plasma by liquid-chromatography–ion trap spectrometry

“…Several gas chromatographic [4,5] and high performance liquid chromatographic (HPLC) [6][7][8][9][10] methods have been described for determination of pseudoephedrine in plasma. The limit of quantitation (LOQ) of HPLC assays ranges from 10 to 50 ng/ml.…”

“…The limit of quantitation (LOQ) of HPLC assays ranges from 10 to 50 ng/ml. Sample preparation is performed by liquid extraction [6,10], solid-phase extraction [7] or column switching [8,9]. Recently, several HPLC [11] and liquid chromatographymass spectrum (LC/MS) [1, 12,13] methods have been reported to detect cetirizine in plasma.…”

Simultaneous determination of chlorpheniramine and pseudoephedrine in human plasma by liquid chromatography–tandem mass spectrometry

“…Chlorpheniramine and pseudoephedrine are commonly combined in one preparation for symptomatic treatment of coughs and the common cold (Yacobi et al, 1980). Some procedures have been described for the assay of either chlorpheniramine or pseudoephedrine in biological fluids, such as GC (Smith et al, 1978), HPLC with radioimmunoassay (Midha et al, 1984), fluorescence (Miyamoto, 1987) or UV (Guo et al, 1999;Bui et al, 2000;Macek et al, 2002) detections. Recently, a liquid chromatographic-tandem mass spectrometric (LC-MS-MS) method (Celma et al, 2000) and a liquid chromatographic-single quadrupole mass spectrometric (LC-MS) method (Takagaki et al, 2002) were reported to determine chlorpheniramine in human plasma.…”