Direct induction of somatic embryogenesis from immature zygotic embryo of cashewnut (Anacardium occidentale L.)

Gogate, Shilpa S.; Nadgauda, R. S.

doi:10.1016/s0304-4238(02)00124-3

Cited by 19 publications

(8 citation statements)

References 19 publications

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“…In the present study, there were no nodules or white protuberance observed in explants cultured without growth regulators. Similar observation was recorded in cashew (Gogate and Nadguda, 2003) whereby explants did not show any embryogenic response or callus formation in medium that was lacked of plant growth regulators. In this study, embryoids like structures were observed and concentrated towards the convex side on the surface and the degree of embryoid formation decreased towards the concave side at the sixth week.…”

Section: Embryoid Formationsupporting

confidence: 83%

In Vitro Response of Cotyledon Explants of Cashew (Anacardium occidentale L.) Cultured in Different Concentrations of BAP

Bavatharine¹,

Seran²

2013

J Food & Agric

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This experiment was carried out to study the embryogenic response of cashew (Anacardium occidentale L.) cotyledon explants cultured in different concentrations of BAP under in vitro conditions. Sterilized cotyledon segments (10 mm long, 5 mm width) were cultured on full strength MS basal medium containing various concentrations (0-10 mg/L) of BAP. The result revealed the medium without BAP exhibited profuse root organogenesis which decreased with the increase in the concentration of BAP. Greenish or yellowish white nodular structures appeared on the edge of the surface concentrated towards the convex side of the explants. Consequently, these structures gave rise to somatic embryoids and the degree of embryoid formation increased with the increasing concentration of BAP. MS medium with 8 mg/L BAP was the most optimal concentration for the induction of somatic embryos among the tested. Cytological study confirmed the development of somatic embryoids directly from cultured cotyledon explants.

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Section: Embryoid Formationsupporting

confidence: 83%

In Vitro Response of Cotyledon Explants of Cashew (Anacardium occidentale L.) Cultured in Different Concentrations of BAP

Bavatharine¹,

Seran²

2013

J Food & Agric

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“…2A). Conversion of somatic embryos in plantlets on medium MS without growth regulators have also been reported for several medicinal species (Rout et al, 2000;TeChato and Rungnoi, 2000;Gogate et al, 2003). In vitro plantlets of yacón with well-developed roots were transferred to non-sterile conditions and acclimated to ambient humidity (Fig.…”

Section: Resultsmentioning

confidence: 79%

“…Requirement of light during in vitro morphogenetic processes has been proved to depend on the species tested. Somatic embryos were induced when explants were cultured either in the presence of light (Fernando et al, 2002;Martin et al, 2000;Martin, 2003) or under dark conditions (Fernando et al, 2001;Gogate et al, 2003). Medium composition affected the embryogenic response of yacón as well.…”

Section: Resultsmentioning

confidence: 99%

Plant regeneration through somatic embryogenesis of yacón [smallanthus sonchifolius (poepp. and endl.) H. Robinson]

Corrêa

Oliveira

Astarita

et al. 2009

Braz. arch. biol. technol.

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“…Jain et al (1995) also reported some difficulties in induction of calli from mature tissue of Picea abies, whereas juvenile tissues seemed more suitable. Other results indicated that zygotic embryos of cashew nut (Anacardium occidentale L.) at different developmental stages showed different potential for somatic embryogenesis (Gogate and Nadgauda, 2003). In further research, the relationship between the physiological-biochemical status of zygotic embryos and the induction of somatic embryos should be investigated.…”

Section: Discussionmentioning

confidence: 99%

Efficient Somatic Embryogenesis and Plant Regeneration from Immature Embryos of Tapiscia sinensis Oliv., an Endemic and Endangered Species in China

Wang¹,

Chen²,

Wang³

et al. 2014

horts

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High-frequency somatic embryogenesis and plant regeneration were achieved from immature cotyledonary-stage embryos in the endangered plant, Tapiscia sinensis Oliv. Plant growth regulators with different concentrations and combinations on embryogenesis capacity were studied. The optimal explants for in vitro somatic embryogenesis were immature embryos in T. sinensis. A high callus induction rate of 100% was achieved on Murashige and Skoog (MS) basal medium supplemented with 1.0 mg·Ll−1 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5% (w/v) activated charcoal. Alternatively, a high induction rate (96.16%) of somatic embryogenesis was obtained on MS basal medium supplemented with the combination of 0.05 mg·L−1 α-naphthaleneacetic acid (NAA) and 0.2 mg·L−1 6-benzylaminopurine (6-BA), and somatic embryos proliferated fastest on the mentioned medium supplemented with 0.5% (w/v) activated charcoal and 3% (w/v) sucrose, inoculation of explants proliferating 21 times in the 23-day subculture. Of the 100 plantlets transferred to field after the acclimation, 95 (95%) survived. Based on the histocytological observations, the development of somatic embryos was similar to that of zygotic embryos. There were two accumulation peaks of starch grains in the embryogenic calli and in the globular-stage embryos, both closely related to the energy supply, and the embryoids were of multicelluar origin.

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Direct induction of somatic embryogenesis from immature zygotic embryo of cashewnut (Anacardium occidentale L.)

Cited by 19 publications

References 19 publications

In Vitro Response of Cotyledon Explants of Cashew (Anacardium occidentale L.) Cultured in Different Concentrations of BAP

In Vitro Response of Cotyledon Explants of Cashew (Anacardium occidentale L.) Cultured in Different Concentrations of BAP

Plant regeneration through somatic embryogenesis of yacón [smallanthus sonchifolius (poepp. and endl.) H. Robinson]

Efficient Somatic Embryogenesis and Plant Regeneration from Immature Embryos of Tapiscia sinensis Oliv., an Endemic and Endangered Species in China

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