Direct imaging of biological sulfur dioxide derivatives in vivo using a two-photon phosphorescent probe

Li, Guanying; Chen, Yu; Wang, Jinquan; Wu, Jingzhi; Gasser, Gilles; Ji, Liang‐Nian; Chao, Hui

doi:10.1016/j.biomaterials.2015.06.014

Cited by 60 publications

(35 citation statements)

References 45 publications

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“…[131] presented the first two-photon phosphorescent probes to detect 11C) suggested the sulfite concentration in the brain is 8.39 ± 0.80 mM. These observations demonstrate that 39d is a reliable and convenient probe for intracellular sulfite determination.…”

Section: Sulfur Dioxide (So 2 )mentioning

confidence: 70%

Two-photon luminescent metal complexes for bioimaging and cancer phototherapy

Chen

Guan

Zhang

et al. 2016

Coordination Chemistry Reviews

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229

108

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Section: Sulfur Dioxide (So 2 )mentioning

confidence: 70%

Two-photon luminescent metal complexes for bioimaging and cancer phototherapy

Chen

Guan

Zhang

et al. 2016

Coordination Chemistry Reviews

Self Cite

229

108

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“…A glutathione-dependent thiosulfate sulfurtransferase (TST) is likely to mediate the subsequent transfer of the sulfane sulfur from thiosulfate to glutathione in a reaction (step 2) that produces glutathione persulfide (GSS − ) and regenerates the sulfite consumed in step 1 28 . TST activity is readily detected in Caenorhabditis elegans , HepG2 cells, and rat liver slices where mitochondrial production of sulfite from thiosulfate and glutathione is observed in a reaction that can be blocked by inactivation of TST 29, 30 . Two human genes ( TSTD1 and TST ) are known to encode enzymes (TSTD1 and rhodanese, respectivly) that exhibit TST activity, as judged by properties observed with the recombinant proteins 1, 28 .…”

mentioning

confidence: 99%

Use of Tissue Metabolite Analysis and Enzyme Kinetics To Discriminate between Alternate Pathways for Hydrogen Sulfide Metabolism

2017

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Hydrogen sulfide (HS) is an endogenously synthesized signaling molecule that is enzymatically metabolized in mitochondria. The metabolism of HS maintains optimal concentrations of the gasotransmitter and produces sulfane sulfur (S)-containing metabolites that may be functionally important in signaling. Sulfide:quinone oxidoreductase (SQOR) catalyzes the initial two-electron oxidation of HS to S using coenzyme Q as the electron acceptor in a reaction that requires a third substrate to act as the acceptor of S. We discovered that sulfite is a highly efficient acceptor and proposed that sulfite is the physiological acceptor in a reaction that produces thiosulfate, a known metabolic intermediate. This model has been challenged by others who assume that the intracellular concentration of sulfite is very low, a scenario postulated to favor reaction of SQOR with a considerably poorer acceptor, glutathione. In this study, we measured the intracellular concentration of sulfite and other metabolites in mammalian tissues. The values observed for sulfite in rat liver (9.2 μM) and heart (38 μM) are orders of magnitude higher than previously assumed. We discovered that the apparent kinetics of oxidation of HS by SQOR with glutathione as the S acceptor reflect contributions from other SQOR-catalyzed reactions, including a novel glutathione:CoQ reductase reaction. We used observed metabolite levels and steady-state kinetic parameters to simulate rates of oxidation of HS by SQOR at physiological concentrations of different S acceptors. The results show that the reaction with sulfite as the S acceptor is a major pathway in liver and heart and provide insight into the potential dynamics of HS metabolism.

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“…125 Early studies indicate that subsequently, its derivatives enter the blood and dissociate to form the derivatives bisulfite and sulfite, 126 both of which have been found in hippocampal slices from rat brain. 127…”

Section: Discussionmentioning

confidence: 99%

The Impact of Inhaled Ambient Ultrafine Particulate Matter on Developing Brain: Potential Importance of Elemental Contaminants

et al. 2019

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Epidemiological studies report associations between air pollution (AP) exposures and several neurodevelopmental disorders including autism, attention deficit disorder, and cognitive delays. Our studies in mice of postnatal (human third trimester brain equivalent) exposures to concentrated ambient ultrafine particles (CAPs) provide biological plausibility for these associations, producing numerous neuropathological and behavioral features of these disorders, including male-biased vulnerability. These findings raise questions about the specific components of AP that underlie its neurotoxicity, which our studies suggest could involve trace elements as candidate neurotoxicants. X-ray fluorescence analyses of CAP chamber filters confirm contamination of AP exposures by multiple elements, including iron (Fe) and sulfur (S). Correspondingly, laser ablation inductively coupled plasma mass spectrometry of brains of male mice indicates marked postexposure elevations of Fe and S and other elements. Elevations of brain Fe and S in particular are consistent with potential ferroptotic, oxidative stress, and altered antioxidant capacity-based mechanisms of CAPs-induced neurotoxicity, supported by observations of increased serum oxidized glutathione and increased neuronal cell death in nucleus accumbens with no corresponding significant increase in caspase-3, in male brains following postnatal CAP exposures. Understanding the role of trace element contaminants of particulate matter AP as a source of neurotoxicity is critical for public health protection.

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Direct imaging of biological sulfur dioxide derivatives in vivo using a two-photon phosphorescent probe

Cited by 60 publications

References 45 publications

Two-photon luminescent metal complexes for bioimaging and cancer phototherapy

Two-photon luminescent metal complexes for bioimaging and cancer phototherapy

Use of Tissue Metabolite Analysis and Enzyme Kinetics To Discriminate between Alternate Pathways for Hydrogen Sulfide Metabolism

The Impact of Inhaled Ambient Ultrafine Particulate Matter on Developing Brain: Potential Importance of Elemental Contaminants

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