Direct ethanol production from cellulosic materials by the hypersaline-tolerant white-rot fungus Phlebia sp. MG-60

Kamei, Ichiro; Hirota, Yoshiyuki; Mori, Toshio; Hirai, Hirofumi; Meguro, Sadatoshi; Kondo, Ryuichiro

doi:10.1016/j.biortech.2012.02.109

Cited by 65 publications

(35 citation statements)

References 28 publications

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“…From this group, Phlebia sp. MG-60 was the only one to exhibit the uncommon characteristic of degrade lignin and produce ethanol from cellulose [73]. This fungal strain has been evaluated in the production of ethanol from different feedstocks such as hardwood kraft pulp, waste newspaper, sugarcane bagasse, and hard wood [73][74][75].…”

Section: Delignificationmentioning

confidence: 99%

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Ligninolytic enzymes: a biotechnological alternative for bioethanol production

Plácido

Capareda

2015

Bioresour. Bioprocess.

179

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Ligninolytic fungi and enzymes (i.e., laccase, manganese peroxidase, and lignin peroxidase) have been applied recently in the production of second-generation biofuels. This review contains the analysis of ligninolytic enzymes and their applications in second-generation biofuels. In here, each of the ligninolytic enzymes was described analyzing their structures, catalysis, and reaction mechanism. Additionally, delignification and detoxification, the two most important applications of ligninolytic enzymes, were reviewed and analyzed. The analysis includes an evaluation of the biochemical process, feedstocks, and the ethanol production. This review describes the current situation of the ligninolytic enzymes technology and its future applications in bioethanol industry.

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Section: Delignificationmentioning

confidence: 99%

“…Integrated fungal fermentation (IFF) is a consolidated process where a fungus or a group of fungi transform biomass into ethanol without the participation of other treatments or microorganisms [73]. Phlebia sp.…”

Section: Delignificationmentioning

confidence: 99%

Ligninolytic enzymes: a biotechnological alternative for bioethanol production

Plácido

Capareda

2015

Bioresour. Bioprocess.

179

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“…The white-rot fungus Phlebia sp. MG-60 was found as a good producer of ethanol from lignocelluloses (Kamei et al 2012b). Kamei et al (2012a) proposed a process of unified aerobic delignification and anaerobic saccharification and fermentation of wood by white-rot fungus Phlebia sp.…”

Section: Introductionmentioning

confidence: 99%

Effect of Biological Pretreatment with White-rot Fungus Trametes hirsuta C7784 on Lignin Structure in Carex meyeriana Kunth

Mao

Zhang

et al. 2013

BioResources

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Carex meyeriana Kunth was subjected to biological pretreatment with the white-rot fungus Trametes hirsuta C7784, and the structural changes of the lignin were investigated. Results showed that there was a slight decrease in carbohydrate content after pretreatment for 3 weeks, but a noticeable decrease in lignin and carbohydrate contents after 6 weeks. Detail structural analysis of the isolated lignin from the samples revealed that Carex meyeriana Kunth lignin consisted mainly of p-hydroxyphenyl (H), guaiacyl (G), and syringyl (S) units with minor p-coumarate and ferulate units. The predominant lignin interunits were β-O-4´ ether linkages, followed by phenylcoumaran, together with a lower portion of resinol and tricin substructures. After pretreatment for 6 weeks, the contents of β-β´ and β-5´ linkages notably decreased, suggesting that the fungus preferentially attacked these linkages. The pretreatment led to an increase of S/G ratio from 0.64 in the control to 0.83 in the sample pretreated for 6 weeks. The comprehensive understanding of the structural changes of lignin offers new insights into the biological degradation of Carex meyeriana Kunth by white-rot fungus.

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“…When this fungus was cultivated with 20 g/l of unbleached hardwood kraft pulp or waste newspaper, 8.4 and 4.2 g/l ethanol were produced after 168 and 216 h of incubation (ethanol yield of 0.42 and 0.20 g/g lignocellulose), respectively. In addition, it was shown that glucose, mannose, galactose, fructose, and xylose were completely assimilated by this strain to give ethanol yields of 0.44, 0.41, 0.40, 0.41, and 0.33 g/g of sugar, respectively (Kamei et al, 2012a). This white-rot fungus was able to selectively degrade lignin, and directly produced ethanol from delignified oak wood under aerobic solid-state fermentation conditions.…”

Section: Flammulina Velutipesmentioning

confidence: 90%

“…It was further reported that these kinds of microorganisms were tolerant to up to 120 g/l ethanol further confirming their suitability for CBP compared to C. thermocellum (Okamura et al, 2001). Kamei et al (2012a) reported that the white rot fungus Phlebiasp. (strain MG 60) was able to convert lignocellulose to ethanol under semi-aerobic conditions and that could be used as a suitable CBP organism.…”

Section: Flammulina Velutipesmentioning

confidence: 99%

Advances in consolidated bioprocessing systems for bioethanol and butanol production from biomass: a comprehensive review

2015

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HIGHLIGHTS Various CBP strategies have been discussed. Recently, lignocellulosic biomass as the most abundant renewable resource has been widely considered for bioalcohols production. However, the complex structure of lignocelluloses requires a multi-step process which is costly and time consuming. Although, several bioprocessing approaches have been developed for pretreatment, saccharification and fermentation, bioalcohols production from lignocelluloses is still limited because of the economic infeasibility of these technologies. This cost constraint could be overcome by designing and constructing robust cellulolytic and bioalcohols producing microbes and by using them in a consolidated bioprocessing (CBP) system. This paper comprehensively reviews potentials, recent advances and challenges faced in CBP systems for efficient bioalcohols (ethanol and butanol) production from lignocellulosic and starchy biomass. The CBP strategies include using native single strains with cellulytic and alcohol production activities, microbial co-cultures containing both cellulytic and ethanologenic microorganisms, and genetic engineering of cellulytic microorganisms to be alcohol-producing or alcohol producing microorganisms to be cellulytic. Moreover, high-throughput techniques, such as metagenomics, metatranscriptomics, next generation sequencing and synthetic biology developed to explore novel microorganisms and powerful enzymes with high activity, thermostability and pH stability are also discussed. Currently, the CBP technology is in its infant stage, and ideal microorganisms and/or conditions at industrial scale are yet to be introduced. So, it is essential to bring into attention all barriers faced and take advantage of all the experiences gained to achieve a high-yield and low-cost CBP process.

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Direct ethanol production from cellulosic materials by the hypersaline-tolerant white-rot fungus Phlebia sp. MG-60

Cited by 65 publications

References 28 publications

Ligninolytic enzymes: a biotechnological alternative for bioethanol production

Ligninolytic enzymes: a biotechnological alternative for bioethanol production

Effect of Biological Pretreatment with White-rot Fungus Trametes hirsuta C7784 on Lignin Structure in Carex meyeriana Kunth

Advances in consolidated bioprocessing systems for bioethanol and butanol production from biomass: a comprehensive review

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