volume 13, issue 6, P268-271 1991
DOI: 10.1111/j.1472-765x.1991.tb00625.x
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Abstract: U. 1991. Direct detection by polymerase chain reaction (PCR) of Escherichia coli in water and soft cheese and identification of enterotoxigenic strains. Letters in Applied Microbiology 13, 268-271.PCR was used to develop a method to detect Escherichia coli in surface water and soft cheese, which does not require cultivation of bacteria. DNA sequences from the malB operon of E. coli were amplified to specifically detect this bacterium. Samples of surface water and soft cheese naturally contaminated with E. col…

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