Direct capture of lactoferrin from cheese whey on supermacroporous column of polyacrylamide cryogel with copper ions

Abstract

Lactoferrin is a protein that is present in cheese whey (a waste product from the dairy industry) and has several biological activities. However, its production from whey must have a high yield and low cost for industrial applications. As such, this study reports the use of polyacrylamide cryogel, loaded with Cu(2+) (through the bond with iminodiacetic acid (IDA)), as an adsorbent for the chromatographic process to capture lactoferrin whey. Ultrafiltered cheese whey was passed through the cryogel-IDA-Cu(2+) system. The eluates were subjected to analysis of total protein, SDS-PAGE and size exclusion chromatography. The results showed an axial dispersion coefficients, at different superficial velocities of liquid, in a range of 10(-6)-10(-5)m(2)/s. The cryogel demonstrated good hydraulic permeability (4.7086×10(-13)m(2)) and a porosity of approximately 78.2%. The IDA-Cu(2+) cryogel system was also able to capture lactoferrin in high purity.

Preparation of an affinity cryogel column for lysozyme purification

“…The produced cryogel monoliths presented a percentage of macropores (porosity, Ø) around 74.1 % ± 0.04 and swelling capacity (S w/w ) of 14.55 ± 0.15 (kg/kg). These values were close to those found by Carvalho et al [34] for polyacrilamide cryogels.…”

“…Hydraulic permeability was equal to 5.9 x 10 -13 m 2 and was close to the values obtained by Yao et al [24] and Carvalho et al [34] for polyacrylamide cryogels. The values of the axial dispersial coefficients (D ax ) were calculated and the results are shown in Fig.…”

“…6. The D ax values varied from 10 -8 to 10 -5 m 2 /s and are similar to the range of values obtained by Yao et al [4,24] , Carvalho et al [34] and Machado et al [5] for supermacroporous cryogels, indicating that axial dispersion is weak in the affinity column.…”

“…These values were between 0.04 mm and 0.05 mm and were less than those obtained by other authors [5,24,34] .…”

Adsorptive behavior of α-lactalbumin on cation-exchange supermacroporous monolithic column

“…Several works have been conducted with the purpose of recovering and purifying whey proteins by different methods [8][9][10][11][12][13]. Ion-exchange chromatography (IEC) has been extensively employed in protein recovery and purification [14][15][16][17][18][19].…”